Journal ArticleDOI
The role of the leucine zipper in the fos–jun interaction
Tony Kouzarides,Edward B. Ziff +1 more
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TLDR
It is shown that the complex between fos and jun can bind to DNA more tightly than either protein alone and that basic residues adjacent to the leucine repeat offos contribute to the DNA-binding potential of the complex.Abstract:
Mutagenesis of the fos protein supports the hypothesis that a heptad repeat of leucine residues stabilizes the interaction between the fos and jun proteins. We show that the complex between fos and jun can bind to DNA more tightly than either protein alone and that basic residues adjacent to the leucine repeat offos contribute to the DNA-binding potential of the complex.read more
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The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation.
Peter Angel,Michael Karin +1 more
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Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins
Pamela J. Mitchell,Robert Tjian +1 more
TL;DR: This review summarizes recent studies that define structural domains for DNA binding and transcriptional activation functions in sequence-specific transcription factors in mammalian DNA binding transcription factors.
Journal ArticleDOI
AP-1 function and regulation.
TL;DR: This work has shown that regulation by heterodimerization between Jun, Fos and ATF proteins, AP-1 activity is regulated through interactions with specific protein kinases and a variety of transcriptional coactivators, and there has been considerable progress in understanding some of the mechanisms and signaling pathways involved in the regulation of AP.
Journal ArticleDOI
Stimulus-Transcription Coupling in the Nervous System: Involvement of the Inducible Proto-Oncogenes fos and jun
James I. Morgan,Tom Curran +1 more
Journal ArticleDOI
The regulation and function of c-fos and other immediate early genes in the nervous system.
TL;DR: This review highlights the importance of identifying the genes that are responsive to trans-synaptic stimulation and membrane electrical activity in neural cells and proposes that IEGs encode regulatory proteins that control the expression of late response genes.
References
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Journal ArticleDOI
Rapid and efficient site-specific mutagenesis without phenotypic selection.
TL;DR: The high efficiency, approximately equal to 10-fold greater than that observed using current methods without enrichment procedures, is obtained by using a DNA template containing several uracil residues in place of thymine, which is applied to mutations introduced via both oligonucleotides and error-prone polymerization.
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Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter
TL;DR: In this paper, a simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure is described, based on the unusually specific RNA synthesis by bacteriophage SP6 RNA polymerase which initiates transcription exclusively at an SP6 promoter.
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The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
TL;DR: A 30-amino-acid segment of C/EBP, a newly discovered enhancer binding protein, shares notable sequence similarity with a segment of the cellular Myc transforming protein, and may represent a characteristic property of a new category of DNA binding proteins.
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Expression of a single transfected cDNA converts fibroblasts to myoblasts.
TL;DR: In this article, the major open reading frame encoded by this cDNA contains a short protein segment similar to a sequence present in the myc protein family, and the expression of one of these cDNAs transfected into C3H10T1/2 fibroblasts, where it is not normally expressed, is sufficient to convert them to stable myoblasts.
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The c-fos protein interacts with c - Jun AP -1 to stimulate transcription of AP-1 responsive genes
TL;DR: Fos is a trans-acting factor that is capable of stimulating gene expression not by direct binding to DNA but by interaction with the sequence-specific transcription factor AP-1, therefore recognition of specific cis-elements by AP- 1 is a prerequisite for Fos-mediated stimulation of gene expression.