Journal ArticleDOI
The Stability of N-Ethylmaleimide and its Reaction with Sulfhydryl Groups
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This article is published in Journal of the American Chemical Society.The article was published on 1955-07-01. It has received 336 citations till now.read more
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Positively charged amino groups on the surface of normal and cancer cells.
TL;DR: The development of a suitably mild chemical treatment of intact live cells using citraconic anhydride (CA) and 2,3-dimethylmaleic anHydride (DMA) at pH 6 · 4 to 6 · 9 at room temperature (ca. 18°C) to block the cell surface amino groups reversibly is described.
Journal ArticleDOI
The Role of Sulfhydryl Groups in the Catalytic Function of Isocitrate Dehydrogenase II. EFFECT OF N-ETHYLMALEIMIDE ON KINETIC PROPERTIES
Roberta F. Colman,Rita Chu +1 more
TL;DR: Dehydrogenase and decarboxylase activity are both lost as a result of incubation of TPN-dependent isocitrate dehydrogenase with N-ethylmaleimide (NEM), and the rate of inactivation is decreased 450-fold by isocite in the presence of MnSO4, indicating a specific reaction at the active site of the enzyme.
Journal ArticleDOI
Label-free colorimetric sensing of ascorbic acid based on Fenton reaction with unmodified gold nanoparticle probes and multiple molecular logic gates.
Li-Pei Zhang,Bo Hu,Jian-Hua Wang +2 more
TL;DR: A label-free strategy based on Fenton reaction with unmodified gold nanoparticles (AuNPs) as probe is demonstrated for ascorbic acid (AA) sensing and a multiple logic gate system includes two logic operations has been designed with small molecules (AA, l-cysteine, glutathione) as inputs and the colorimetric changes of AuNPs solution as outputs.
Journal ArticleDOI
A Flexible Method for the Stable, Covalent Immobilization of Enzymes at Electrode Surfaces
Firas A. Al-Lolage,Firas A. Al-Lolage,Marta Meneghello,Su Ma,Roland Ludwig,Philip N. Bartlett +5 more
TL;DR: This work establishes a stable, site-specific immobilization of redox proteins and enzymes by using site-directed mutagenesis to introduce cysteine residues at specific locations on the protein surface and the reaction between the free thiol group and maleimide groups attached to the electrode surface to immobilize the mutated enzymes.