Journal ArticleDOI
The Stability of N-Ethylmaleimide and its Reaction with Sulfhydryl Groups
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This article is published in Journal of the American Chemical Society.The article was published on 1955-07-01. It has received 336 citations till now.read more
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Thiolate and phosphorothioate functionalized fluoresceins and their use as fluorescent labels.
TL;DR: A high degree of conjugation control is achieved in that modulation of the stoichiometry of the label and enzyme results in incorporation from seven to eight fluorophores per protein, depending on the ratio of the phosphorothioate fluorescein to alkaline phosphatase.
Journal ArticleDOI
Catalytic Performance of a Class III Old Yellow Enzyme and Its Cysteine Variants.
Anika Scholtissek,Eric Gädke,Caroline E. Paul,Caroline E. Paul,Adrie H. Westphal,Willem J. H. van Berkel,Dirk Tischler +6 more
TL;DR: It is established that the generation of a cysteine-free enzyme and cofactor optimization allows the development of more robust class III OYEs.
Journal ArticleDOI
Nonpolar interactions in the modification of an essential sulfhydryl of sorbitol dehydrogenase by N-alkylmaleimides.
TL;DR: Protection of the enzyme from N-alkylmaleimide inactivation was observed with the binding of NADH, whereas both NAD and sorbitol were ineffective as protecting ligands.
Journal ArticleDOI
The kinetics of the reaction of N-ethylmaleimide with denatured β-lactoglobulin and ovalbumin
James G. Franklin,J. Leslie +1 more
TL;DR: The kinetics of the reaction of β-lactoglobulin with N-ethylmaleimide have been studied in order to relate the reactivity of the sulfhydryl groups to the structure of the protein in urea and sodium dodecyl sulfate solutions and the reaction does not follow simple second-order kinetics.
Journal ArticleDOI
The Internal Hydrogen Ion Concentration of Thiobacillus thio-oxidans and Survival after Irradiation
D. L. Dewey,Jean Beecher +1 more
TL;DR: If biological damage is due exclusively to H and/or OH radical attack, and if the hydrated electron oxygen complex is responsible for the increased biological effectiveness of ionizing radiation in the presence of oxygen, then this effect could be reduced or eliminated at low pH values.