Journal ArticleDOI
The structure of a virus-encoded nucleosome
Marco Igor Valencia-Sánchez,Stephen Abini-Agbomson,Miao Wang,Rachel Lee,Nikita Vasilyev,Nikita Vasilyev,Jenny Zhang,Pablo De Ioannes,Bernard La Scola,Paul B. Talbert,Paul B. Talbert,Steve Henikoff,Steve Henikoff,Evgeny Nudler,Evgeny Nudler,Albert Erives,Karim-Jean Armache +16 more
TLDR
The authors showed that fused histone pairs Hβ-Hα and Hδ-Hγ from Marseillevirus are structurally analogous to the eukaryotic histone pair H2B-H2A and H4-H3.Abstract:
Certain large DNA viruses, including those in the Marseilleviridae family, encode histones. Here we show that fused histone pairs Hβ-Hα and Hδ-Hγ from Marseillevirus are structurally analogous to the eukaryotic histone pairs H2B-H2A and H4-H3. These viral histones form 'forced' heterodimers, and a heterotetramer of four such heterodimers assembles DNA to form structures virtually identical to canonical eukaryotic nucleosomes.read more
Citations
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Posted ContentDOI
A phylogenomic framework for charting the diversity and evolution of giant viruses.
TL;DR: In this article, a comprehensive phylogenomic analysis of the Nucleocytoviricota was performed and a set of giant virus orthologous groups (GVOGs) together with a benchmarked reference phylogeny was delineated.
Journal ArticleDOI
Virus-encoded histone doublets are essential and form nucleosome-like structures.
Yang Liu,Yang Liu,Hugo Bisio,Chelsea Marie Toner,Sandra Jeudy,Nadège Philippe,Keda Zhou,Samuel Bowerman,Alison E. White,Garrett B. Edwards,Chantal Abergel,Karolin Luger,Karolin Luger +12 more
TL;DR: The authors showed that doublet histones are essential for viral infectivity, localize to cytoplasmic viral factories after virus infection, and ultimately are found in the mature virions.
Journal ArticleDOI
Nuclear cGAS: guard or prisoner?
TL;DR: In this article, the authors discuss the biological implications of nuclear cGAS and its interaction with chromatin, including various mechanisms for n-cGAS inhibition, release of chromatin-bound c-GAS, regulation of different c-gAS pools in the cell, and chromatin structure/chromatin protein effects on n-CGAS activation leading to cgAS-induced autoimmunity.
Journal ArticleDOI
Clandestinovirus: A Giant Virus With Chromatin Proteins and a Potential to Manipulate the Cell Cycle of Its Host Vermamoeba vermiformis.
Clara Rolland,Julien Andreani,Dehia Sahmi-Bounsiar,Mart Krupovic,Bernard La Scola,Anthony Levasseur,Anthony Levasseur +6 more
TL;DR: The clandestinovirus as discussed by the authors has a linear dsDNA genome of 581,987 base pairs containing 617 genes, which is 65% larger than that of medusavirus, emphasizing the considerable genome size variation within this virus family.
Journal ArticleDOI
The genomes of nucleocytoplasmic large DNA viruses: viral evolution writ large.
TL;DR: The origin and evolution of these unusually complex viruses has been puzzling, and various mechanisms have been put forward to explain how some NCLDVs could have reached genome sizes and coding capacity overlapping with those of cellular microbes as mentioned in this paper.
References
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Eric F. Pettersen,Thomas D. Goddard,Conrad C. Huang,Gregory S. Couch,Daniel M. Greenblatt,Elaine C. Meng,Thomas E. Ferrin +6 more
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Phenix - a comprehensive python-based system for macromolecular structure solution
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TL;DR: PHENIX has been developed to provide a comprehensive system for macromolecular crystallographic structure solution with an emphasis on the automation of all procedures.
Journal ArticleDOI
Crystal structure of the nucleosome core particle at 2.8 Å resolution
TL;DR: The X-ray crystal structure of the nucleosome core particle of chromatin shows in atomic detail how the histone protein octamer is assembled and how 146 base pairs of DNA are organized into a superhelix around it.
Journal ArticleDOI
MotionCor2: Anisotropic Correction of Beam-Induced Motion for Improved Cryo-Electron Microscopy
Shawn Q. Zheng,Eugene Palovcak,Jean Paul Armache,Kliment A. Verba,Yifan Cheng,David A. Agard +5 more
TL;DR: MotionCor2 software corrects for beam-induced sample motion, improving the resolution of cryo-EM reconstructions.