The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes.
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In this article, the authors analyzed the transcriptional response to osmotic shock in the yeast Saccharomyces cerevisiae and found that the mRNA level of 186 genes increased at least 3-fold after a shift to NaCl or sorbitol whereas that of more than 100 genes was at least 1.5-fold diminished.About:
This article is published in Journal of Biological Chemistry.The article was published on 2000-03-24 and is currently open access. It has received 557 citations till now. The article focuses on the topics: Osmotic concentration & Osmotic shock.read more
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Salt and drought stress signal transduction in plants
TL;DR: Salt and drought stress signal transduction consists of ionic and osmotic homeostasis signaling pathways, detoxification (i.e., damage control and repair) response pathways, and pathways for growth regulation.
Journal ArticleDOI
Genomic expression programs in the response of yeast cells to environmental changes.
Audrey P. Gasch,Paul T. Spellman,Camilla M. Kao,Orna Carmel-Harel,Michael B. Eisen,Gisela Storz,David Botstein,Patrick O. Brown +7 more
TL;DR: Analysis of genomic expression patterns in the yeast Saccharomyces cerevisiae implicated the transcription factors Yap1p, as well as Msn2p and Msn4p, in mediating specific features of the transcriptional response, while the identification of novel sequence elements provided clues to novel regulators.
Journal ArticleDOI
Osmotic Stress Signaling and Osmoadaptation in Yeasts
TL;DR: An integrated understanding of osmoadaptation requires not only knowledge of the function of many uncharacterized genes but also further insight into the time line of events, their interdependence, their dynamics, and their spatial organization as well as the importance of subtle effects.
Journal ArticleDOI
Transcriptome Changes for Arabidopsis in Response to Salt, Osmotic, and Cold Stress
TL;DR: Together results from all three stresses identified 2,409 genes with a greater than 2-fold change over control, suggesting that about 30% of the transcriptome is sensitive to regulation by common stress conditions, and supporting the hypothesis that an important function of the circadian clock is to “anticipate” predictable stresses such as cold nights.
PatentDOI
IMAGING INDIVIDUAL mRNA MOLECULES USING MULTIPLE SINGLY LABELED PROBES
Arjun Raj,Sanjay Tyagi +1 more
TL;DR: In this article, a method for probing a target sequence of messenger ribonucleic acid molecules (mRNA's) in a fixed, permeabilized cell, including at least 30 non- overlapping probe binding regions of 15-100 nucleotides, was proposed.
References
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Living with water stress: evolution of osmolyte systems
TL;DR: Osmolyte compatibility is proposed to result from the absence of osmolytes interactions with substrates and cofactors, and the nonperturbing or favorable effects of oSMolytes on macromolecular-solvent interactions.
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Elevated recombination rates in transcriptionally active DNA.
TL;DR: Northern analysis of strains containing plasmid inserts with various promoter mutations suggests that the stimulation in recombination is mediated by events initiating within the integrated plasmID sequences.
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An osmosensing signal transduction pathway in yeast.
TL;DR: A signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment is defined, including a rapid, PBS2-dependent tyrosine phosphorylation of HOG1 protein.
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A two-component system that regulates an osmosensing MAP kinase cascade in yeast
TL;DR: A two-component system in Saccharomyces cerevisiae that regulates an osmosensing MAP kinase cascade is described that contains an aspartate residue in the receiver domain of a cognate response regulator molecule.
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The saccharomyces cerevisiae zinc finger proteins msn2p and msn4p are required for transcriptional induction through the stress-response element (stre )
María Teresa Martínez-Pastor,G Marchler,Christoph Schüller,Aron Marchler-Bauer,H Ruis,F Estruch +5 more
TL;DR: The results suggest that MSN2 and MSN4 encode a DNA‐binding component of the stress responsive system and it is likely that they act as positive transcription factors.