To see or not to see: lateral organization of biological membranes and fluorescence microscopy.
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TLDR
The methodological aspects of the aforementioned experimental approaches, particularly stressing the information obtained by the use of UV excited fluorescent probes using two-photon excitation fluorescence microscopy, are discussed.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 2006-10-31 and is currently open access. It has received 366 citations till now. The article focuses on the topics: Two-photon excitation microscopy & Photoactivated localization microscopy.read more
Citations
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Solvatochromic and Fluorogenic Dyes as Environment-Sensitive Probes: Design and Biological Applications
TL;DR: Overall, solvatochromic and fluorogenic probes enable background-free bioimaging in wash-free conditions as well as quantitative analysis when combined with advanced microscopy, such as fluorescence lifetime (FLIM) and ratiometric imaging.
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Fluorescent Analogs of Biomolecular Building Blocks: Design, Properties, and Applications
TL;DR: This article focuses on designing fluorescent probes for the four major families of macromolecular building blocks discussed above, and discusses emissive carbohydrate derivatives, followed byEmissive amino acids, the building blocks of nucleic acids.
Journal ArticleDOI
Current perspectives in pulmonary surfactant--inhibition, enhancement and evaluation.
Yi Y. Zuo,Ruud A. W. Veldhuizen,A. Wilhelm Neumann,Nils O. Petersen,Nils O. Petersen,Fred Possmayer,Fred Possmayer +6 more
TL;DR: A better understanding of the biophysical properties of functional and dysfunctional PS is important for the further development of surfactant therapy, especially for its potential application in ARDS.
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Core-substituted naphthalenediimides
TL;DR: Research of core-substituted naphthalenediimides (cNDIs) is reviewed in a comprehensive yet easily readable manner with emphasis on the ability of cNDIs with electron donating substituents to absorb and fluoresce in all colors without global structural changes.
Journal ArticleDOI
Structure of pulmonary surfactant membranes and films: the role of proteins and lipid-protein interactions.
TL;DR: The limitations in structural complexity and biophysical performance of surfactant preparations reconstituted in vitro will be highlighted in particular, to allow for a proper evaluation of the significance of the experimental model systems used so far to study structure-function relationships in surfactants.
References
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Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
The fluid mosaic model of the structure of cell membranes.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.
Book ChapterDOI
THE FLUID MOSAIC MODEL OF THE STRUCTURE OF CELL MEMBRANES Reprinted with permission from Science, Copyright AAA, 18 February 1972, Volume 175, pp. 720–731.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.
Journal ArticleDOI
Model systems, lipid rafts, and cell membranes.
Kai Simons,Winchil L.C. Vaz +1 more
TL;DR: This review critically analyzes what is known of phase behavior and liquid-liquid immiscibility in model systems and compares these data with what isknown of domain formation in cell membranes.
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