Journal ArticleDOI
Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements.
Arthur Günzl,Elisabetta Ullu,Marion Dörner,Stenio Perdigão Fragoso,Karl F. Hoffmann,Jeffrey D. Milner,Yasuhiro Morita,E. K. Nguu,Stepanka Vanacova,Stefan Wünsch,Amos O. Dare,Helen L. Kwon,Christian Tschudi +12 more
Reads0
Chats0
TLDR
An analysis of the promoter architecture of the SL RNA gene revealed that regulatory elements are located upstream of the coding region and that theSL sequence, in contrast to the nematode SL sequence, is not required for T. brucei SLRNA gene transcription.About:
This article is published in Molecular and Biochemical Parasitology.The article was published on 1997-03-01. It has received 78 citations till now. The article focuses on the topics: RNA polymerase II & Transcription (biology).read more
Citations
More filters
Journal ArticleDOI
trans and cis Splicing in Trypanosomatids: Mechanism, Factors, and Regulation
TL;DR: Studies from the ongoing genome project suggest that the entire chromosome may be transcribed as large transcripts, but not the entire genome itself.
Journal ArticleDOI
RNA polymerase I transcribes procyclin genes and variant surface glycoprotein gene expression sites in Trypanosoma brucei.
Arthur Günzl,Thomas Bruderer,Gabriele Laufer,Bernd Schimanski,Lan-Chun Tu,Hui-Min Chung,Pei-Tseng Lee,Mary Gwo-Shu Lee +7 more
TL;DR: These studies unequivocally show that T. brucei harbors a multifunctional RNA pol I which, in addition to transcribing rDNA, transcribes procyclin genes and VSG gene expression sites.
Journal ArticleDOI
Highly Efficient Tandem Affinity Purification of Trypanosome Protein Complexes Based on a Novel Epitope Combination
TL;DR: Empirope substitution improved purification in experiments by eliminating the inefficiency of calmodulin affinity chromatography and by providing an alternative way of elution using the ProtC peptide in cases where EGTA inactivated protein function.
Journal ArticleDOI
Transcription in kinetoplastid protozoa: why be normal?
TL;DR: A broad overview of what is known about transcription in the kinetoplastids and what has yet to be determined is presented.
Journal ArticleDOI
Trans-splicing in trypanosomes: machinery and its impact on the parasite transcriptome
TL;DR: Recent findings in the field focusing on SL RNA transcription, cap modifications and pseudouridylation are summarized, suggesting that gene regulation, especially during cycling between the two hosts of the parasite, involves alternative trans-splicing.
References
More filters
Journal ArticleDOI
Mass spectrometry of mRNA cap 4 from trypanosomatids reveals two novel nucleosides.
TL;DR: The conservation of cap 4 between these divergent genera suggests that this structure may be common to most if not all Kinetoplastida.
Journal ArticleDOI
Trypanosome mRNAs have unusual cap 4 structures acquired by addition of a spliced leader
TL;DR: It is found that within a population of purified SL RNAs are species that are capped but incompletely modified, which supports the hypothesis that all T. brucei mRNAs share a common leader sequence and indicates that transcription begins four nucleotides upstream of the reported 5' end.
Journal ArticleDOI
Tagetitoxin: a new inhibitor of eukaryotic transcription by RNA polymerase III.
TL;DR: It is demonstrated that tagetitoxin, a bacterial phytotoxin, preferentially inhibits eukaryotic RNA polymerase III, and promoter-directed transcription of cloned genes in cell-free extracts was used to compare tageitoxin inhibition of RNA polymerases from diverse sources.
Journal ArticleDOI
Tandem repetition of the 5′ mini-exon of variant surface glycoprotein genes: A multiple promoter for VSG gene transcription?
Titia de Lange,Alvin Y.C. Liu,Piet Borst,M. Tromp,Jacques H. van Boom,Lex H.T. Van der Ploeg +5 more
TL;DR: A model in which the arrays of mini-exon repeats function as a repetitive promoter for efficient transcription of VSG genes is proposed.
Journal ArticleDOI
Alpha-amanitin resistant transcription of protein coding genes in insect and bloodstream form Trypanosoma brucei.
TL;DR: Comparison of the alpha‐amanitin resistantly transcribed PARP and VSG gene families shows that they both produce one of the most abundant mRNAs [1‐3% of poly(A)+] and they both encode the major cell surface proteins of their respective life cycle stages.
Related Papers (5)
Trypanosome spliced leader RNA genes contain the first identified RNA polymerase II gene promoter in these organisms
Gwen Gilinger,Vivian Bellofatto +1 more