Transcriptionally active genome regions are preferred targets for retrovirus integration.
TLDR
The results strongly suggest that transcriptionally active genome regions are preferred targets for retrovirus integration in mouse fibroblasts.Abstract:
We have analyzed the transcriptional activity of cellular target sequences for Moloney murine leukemia virus integration in mouse fibroblasts. At least five of the nine random, unselected integration target sequences studied showed direct evidence for transcriptional activity by hybridization to nuclear run-on transcripts prepared from uninfected cells. At least four of the sequences contained multiple recognition sites for several restriction enzymes that cut preferentially in CpG-rich islands, indicating integration into 5' or 3' ends or flanking regions of genes. Assuming that only a minor fraction (less than 20%) of the genome is transcribed in mammalian cells, we calculated the probability that this association of retroviral integration sites with transcribed sequences is due to chance to be very low (1.6 x 10(-2]. Thus, our results strongly suggest that transcriptionally active genome regions are preferred targets for retrovirus integration.read more
Citations
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HIV-1 Integration in the Human Genome Favors Active Genes and Local Hotspots
Astrid R. W Schroder,Paul Shinn,Huaming Chen,Charles C. Berry,Joseph R. Ecker,Frederic D. Bushman +5 more
TL;DR: Global analysis of cellular transcription indicated that active genes were preferential integration targets, particularly genes that were activated in cells after infection by HIV-1, and this data suggests how selective targeting promotes aggressive HIV replication.
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Transcription Start Regions in the Human Genome Are Favored Targets for MLV Integration
TL;DR: Map of retroviral integrations in the human genome showed that MLV preferred integration near the start of transcriptional units (either upstream or downstream) whereas HIV-1 preferred integration anywhere in the transcriptional unit but not upstream of a transcriptional start.
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Targeted integration of adeno-associated virus (AAV) into human chromosome 19.
TL;DR: This non‐pathogenic parvovirus thus appears to establish viral latency by integrating its DNA specifically into one chromosomal region, so far unique among the eukaryotic DNA viruses.
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HIV/AIDS epidemiology, pathogenesis, prevention, and treatment
TL;DR: This Seminar provides an update on epidemiology, pathogenesis, treatment, and prevention interventions pertinent to HIV-1.
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Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5
TL;DR: The two-hybrid system was used to identify a human gene product that binds tightly to the human immunodeficiency virus-type 1 (HIV-1) integrase in vitro and stimulates its DNA-joining activity, suggesting that the protein is a human homolog of yeast SNF5, a transcriptional activator required for high-level expression of many genes.
References
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