Transcriptomic Analysis Reveals the Molecular Mechanisms of Drought-Stress-Induced Decreases in Camellia sinensis Leaf Quality.
Weidong Wang,Huahong Xin,Mingle Wang,Qingping Ma,Le Wang,Najeeb A. Kaleri,Yuhua Wang,Xinghui Li +7 more
TLDR
In this study, the effects of drought stress on the phenotype, physiological characteristics and major bioactive ingredients accumulation of C. sinensis leaves were examined, and the results indicated that drought stress resulted in dehydration and wilt of the leaves, and significant decrease in the total polyphenols and free amino acids and increase in thetotal flavonoids.Abstract:
The tea plant [Camellia sinensis (L.) O. Kuntze] is an important commercial crop rich in bioactive ingredients, especially catechins, caffeine, theanine and other free amino acids, which the quality of tea leaves depends on. Drought is the most important environmental stress affecting the yield and quality of this plant. In this study, the effects of drought stress on the phenotype, physiological characteristics and major bioactive ingredients accumulation of C. sinensis leaves were examined, and the results indicated that drought stress resulted in dehydration and wilt of the leaves, and significant decrease in the total polyphenols and free amino acids and increase in the total flavonoids. In addition, HPLC analysis showed that the catechins, caffeine, theanine and some free amino acids in C. sinensis leaves were significantly reduced in response to drought stress, implying that drought stress severely decreased the quality of C. sinensis leaves. Furthermore, differentially expressed genes (DEGs) related to amino acid metabolism and secondary metabolism were identified and quantified in C. sinensis leaves under drought stress using high-throughput Illumina RNA-Seq technology, especially the key regulatory genes of the catechins, caffeine, and theanine biosynthesis pathways. The expression levels of key regulatory genes were consistent with the results from the HPLC analysis, which indicate a potential molecular mechanism for the above results. Taken together, these data provide further insights into the mechanisms underlying the change in the quality of C. sinensis leaves under environmental stress, which involve changes in the accumulation of major bioactive ingredients, especially catechins, caffeine, theanine and other free amino acids.read more
Citations
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Exogenous abscisic acid induces the lipid and flavonoid metabolism of tea plants under drought stress.
Zhongshuai Gai,Yu Wang,Yiqian Ding,Wenjun Qian,Chen Qiu,Hui Xie,Litao Sun,Zhongwu Jiang,Qingping Ma,Linjun Wang,Zhaotang Ding +10 more
TL;DR: The results suggested that the exogenous ABA could alleviate the damages of tea leaves under drought stress through inducing the expression of the genes and altering the contents of metabolites in response to drought stress.
Journal ArticleDOI
Metabolite profiling and transcriptomic analyses reveal an essential role of UVR8-mediated signal transduction pathway in regulating flavonoid biosynthesis in tea plants ( Camellia sinensis ) in response to shading
Linlin Liu,Yingying Li,Guangbiao She,Xianchen Zhang,Brian R. Jordan,Qi Chen,Jian Zhao,Xiaochun Wan +7 more
TL;DR: It is proposed that catechin biosynthesis in C. sinensis leaves is predominantly regulated by UV through the UVR8-mediated signal transduction pathway to MYB12/MYB4 downstream effectors, to modulate flavonoid accumulation.
Journal ArticleDOI
Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant ( Camellia sinensis )
TL;DR: The identified key genes and regulatory network improve the understanding of the molecular mechanism of N usage and offer gene targets for plant improvement.
Journal ArticleDOI
Identification of drought-responsive miRNAs and physiological characterization of tea plant ( Camellia sinensis L.) under drought stress
Yuqiong Guo,Shanshan Zhao,Chen Zhu,Xiaojun Chang,Chuan Yue,Zhong Wang,Lin Yuling,Lai Zhongxiong +7 more
TL;DR: The results of study revealed that the expressions of phase-specific miRNAs vary with morphological, physiological, and biochemical changes, which will be useful for research on drought resistance and provide insights into the mechanisms of drought adaptation and resistance in C. sinensis.
Journal ArticleDOI
Global Transcriptional Analysis Reveals the Complex Relationship between Tea Quality, Leaf Senescence and the Responses to Cold-Drought Combined Stress in Camellia sinensis.
TL;DR: Drought-induced leaf senescence was severely delayed by modulation of a number ofsenescence-associated genes and cold responsive genes, enhancement of antioxidant capacity, and attenuation of lipid degradation, as well as regulation of secondary metabolism pathways that significantly influence the quality of tea during combined stress.
References
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