Journal ArticleDOI
Transformation of bacteria with plasmid DNA by electroporation.
Stefan Fiedler,Reinhard Wirth +1 more
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The possibility of electric field-mediated transformation ("electroporation") of a gram-positive bacterium and two gram-negative bacteria with plasmid DNA was investigated and results are given.About:
This article is published in Analytical Biochemistry.The article was published on 1988-04-01. It has received 186 citations till now. The article focuses on the topics: Plasmid preparation & Transformation efficiency.read more
Citations
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Journal ArticleDOI
Theory and in vivo application of electroporative gene delivery.
Stella Somiari,Jill Glasspool-Malone,Joseph J. Drabick,Richard Gilbert,Richard Heller,Mark J. Jaroszeski,Robert W. Malone +6 more
TL;DR: An overview of the theory and practice of electroporative gene transfer is provided to aid researchers that wish to employ the method for preclinical and translational gene therapy, NAV, and functional genomic research.
Journal ArticleDOI
Improved electroporation and cloning vector system for gram-positive bacteria.
TL;DR: A protocol for transformation of intact Enterococcus faecalis cells by electroporation was developed through a systematic examination of the effects of changes in various parameters, including growth conditions, size, concentration, and purity of DNA used for transformation; and conditions used to select for transformants.
Journal ArticleDOI
Flow cytometry of Escherichia coli on microfluidic devices.
TL;DR: Flow cytometry of the bacterium Escherichia coli was demonstrated on a microfabricated fluidic device (microchip) and the observed labeling efficiencies for the dyes and antibody were greater than 94%.
Book ChapterDOI
Genetic manipulation of pathogenic streptococci.
Michael G. Caparon,June R. Scott +1 more
TL;DR: This chapter discusses genetic manipulation of pathogenic streptococci, which is one of the major causes of morbidity and mortality among debilitated individuals despite the widespread use of antibiotics.
Journal ArticleDOI
Transdermal iontophoresis: combination strategies to improve transdermal iontophoretic drug delivery.
TL;DR: An overview of the use of iontophoresis alone and in conjunction with other approaches such as chemical enhancement, electroporation, sonophores is, and use of microneedles and ion-exchange materials is presented.
References
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Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
A complementation analysis of the restriction and modification of DNA in Escherichia coli.
TL;DR: Intercistronic complementation was observed between three classes of restriction and modification mutants of E. coli B, indicating that at least three cistron (the ram cistrons) are involved in the genetic control of the [restriction and modification of DNA].
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.
Journal ArticleDOI
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.
Shing Chang,Stanley N. Cohen +1 more
TL;DR: The procedure, which involves polyethylene glycolinduced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4x107 transformants per μg of supercoiled DNA.