Journal ArticleDOI
Transition of somatic plant cells to an embryogenic state
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TLDR
This review summarizes relevant experimental observations that can contribute to the description and definition of a transitional state of somatic cells induced to form totipotent, embryogenic cells.Abstract:
Under appropriate in vivo or in vitro conditions, certain somatic plant cells have the capability to initiate embryogenic development (somatic embryogenesis). Somatic embryogenesis provides an unique experimental model to understand the molecular and cellular bases of developmental plasticity in plants. In the last few years, the application of modern experimental techniques, as well as the characterization of Arabidopsis embryogenesis mutants, have resulted in the accumulation of novel data about the acquisition of embryogenic capabilities by somatic plant cells. In this review, we summarize relevant experimental observations that can contribute to the description and definition of a transitional state of somatic cells induced to form totipotent, embryogenic cells. During this somatic-to-embryogenic transition, cells have to dedifferentiate, activate their cell division cycle and reorganize their physiology, metabolism and gene expression patterns. The roles of stress, endogenous growth regulators and chromatin remodelling in the coordinated reorganization of the cellular state are especially emphasized.read more
Citations
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Ploidy instability in long-term in vitro cultures of Coffea arabica L. monitored by flow cytometry
Wellington Ronildo Clarindo,Carlos Roberto Ribeiro de Carvalho,Maria Andréia Corrêa Mendonça +2 more
TL;DR: Flow cytometry was an important tool to detect SV at an early stage of tissue culture in this species, proving to be very useful for quality control in clonal propagation and in the introduction of somaclones to breeding programs.
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Primary callus induction, somatic embryogenesis and regeneration studies in selected elite wheat varieties from pakistan
Mehmooda Munazir,Rahmatullah Qureshi,Ghulam Muhammad Ali,Umer Rashid,Sabahat Noor,Khalid Mehmood,Shoukat Ali,Muhammad Arshad +7 more
TL;DR: Effects of varying concentrations of growth regulators including 2, 4-D, BAP, IAA, Kin were investigated for primary callus induction, embryogenic callus formation and regeneration of two elite wheat varieties viz., Sahar and GA-02 to help in understanding the response of wheat to tissue culture conditions and ultimately promise in improving yield by employing various biotechnological techniques.
Book ChapterDOI
Plant Tissue Culture
Víctor M. Loyola-Vargas,Clelia De-la-Peña,Rosa M. Galaz-Ávalos,Francisco Roberto Quiroz-Figueroa +3 more
TL;DR: PTC technology also explores conditions that promote cell division and genetic re-program-ming in in vitro conditions and it is considered an important tool in both basic and applied studies, as well as in commercial application.
Journal ArticleDOI
Stress-related function of bHLH109 in somatic embryo induction in Arabidopsis.
TL;DR: The results suggest that in SE bHLH109 acts as an activator of ECP63, a member of the LEA (LATE EMBRYOGENESIS ABUNDANT) family, which results in the enhanced tolerance of the cells to stress.
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