UDP-rhamnose:flavanone-7-O-glucoside-2''-O-rhamnosyltransferase. Purification and characterization of an enzyme catalyzing the production of bitter compounds in citrus.
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TLDR
The rhamnosyltransferase catalyzing the production of the bitter flavanone-glucosides, naringin and neohesperidin, was purified to homogeneity, the first complete purification of a rhamnose-transferase, and the isolation of the gene may enable its use in genetic engineering directed to modifying grapefruit bitterness.About:
This article is published in Journal of Biological Chemistry.The article was published on 1991-11-05 and is currently open access. It has received 78 citations till now. The article focuses on the topics: Prunin & Naringin.read more
Citations
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Glycosyltransferases in plant natural product synthesis: characterization of a supergene family
TL;DR: Results obtained with several recombinant enzymes indicate that many glycosyltransferases are regioselective or regiospecific rather than highly substrate specific, which might indicate how plants evolve novel secondary products.
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UGT73C6 and UGT78D1, glycosyltransferases involved in flavonol glycoside biosynthesis in Arabidopsis thaliana.
TL;DR: The present results suggest that UGT78D1 and UGT73C6 should be classified as UDP-rhamnose:flavonol-3-Orhamnosyltransferase and UDP-glucose: FLAVonol -3-O-glycoside-7-O -glucosyl transferase, respectively.
Biosynthesis of flavonoids
Werner Heller,Gert Forkmann +1 more
TL;DR: A rapid and substantial progress to a broad understanding of flavonoid biosynthesis marked the previous reviews of this series of books (Hahlbrock and Grisebach, 1975; Ebel and Hahlburck, 1982; Heller and Forkmann, 1988), and several other reviews have also appeared (Harborne, 1988; Zaprometov, 1989; Stafford, 1990).
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Cloning and characterization of Vitis vinifera UDP-glucose:flavonoid 3-O-glucosyltransferase, a homologue of the enzyme encoded by the maize Bronze-1 locus that may primarily serve to glucosylate anthocyanidins in vivo.
Christopher M. Ford,Christopher M. Ford,Paul K. Boss,Paul K. Boss,Paul K. Boss,Peter B. Høj,Peter B. Høj,Peter B. Høj +7 more
TL;DR: The cloning and optimized expression at 16 °C and the characterization of a Vitis vinifera UDP-glucose:flavonoid 3-O-glauosyltransferase, an enzyme responsible for a late step in grapevine anthocyanin biosynthesis, indicated that the principal, if not only, role of this enzyme is to glucosylate Anthocyanidins in red fruit during ripening.
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Plant Nucleotide Sugar Formation, Interconversion, and Salvage by Sugar Recycling
TL;DR: The importance of the salvage of sugars released from glycans for the formation of nucleotide sugars is emphasized and how recent studies combining biochemical, genetic, molecular and cellular approaches have led to an increased appreciation of the role nucleotide sugar in all aspects of plant growth and development is outlined.
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
TL;DR: A new modification of silver staining is presented which utilizes two chemical properties of thiosulfate: image enhancement by pretreatment of fixed gels, and formation of soluble silver complexes which prevents unspecific background staining during image development.
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Aniline Hydrogen Phthalate as a Spraying Reagent for Chromatography of Sugars
TL;DR: The use of ammoniacal silver nitrate solution as a spraying reagent for revealing the presence of sugars on filter-paper chromatograms has the advantage of general application but has a corresponding disadvantage in reacting with a very wide range of reducing substances other than the sugars, including various impurities commonly present in such solvents as phenol and collidine.
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Quantitative survey of narirutin naringin hesperidin and neohesperidin in citrus
TL;DR: Dosage systematique par chromatographie liquide haute performance, de ces constituants flavonoides chez 52 cultivars d'agrumes appartenant a 4 especes (orange, tangerine, pamplemousse, orange amere) and quelques hybrides, en vue d'un essai de classement chimiotaxonomique et en relation avec l'identification de la purete des jus de fruits as mentioned in this paper.