Journal ArticleDOI
Ultrasensitive Label- and PCR-Free Genome Detection Based on Cooperative Hybridization of Silicon Nanowires Optical Biosensors
Antonio Alessio Leonardi,Maria Josè Lo Faro,Salvatore Petralia,Barbara Fazio,Paolo Musumeci,Sabrina Conoci,Alessia Irrera,Francesco Priolo,Francesco Priolo +8 more
TLDR
The Si NWs sensor showed high sensitivity and specificity, easy detection method, and low manufacturing cost fully compatible with standard silicon process technology, key factors for the future development of a new class of genetic point-of-care devices that are reliable, fast, low cost, and easy to use for self-testing including in the developing countries.Abstract:
The realization of an innovative label- and PCR-free silicon nanowires (NWs) optical biosensor for direct genome detection is demonstrated. The system is based on the cooperative hybridization to selectively capture DNA and on the optical emission of quantum confined carriers in Si NWs whose quenching is used as detection mechanism. The Si NWs platform was tested with Hepatitis B virus (HBV) complete genome and it was able to reach a Limit of Detection (LoD) of 2 copies/reaction for the synthetic genome and 20 copies/reaction for the genome extracted from human blood. These results are even better than those obtained with the gold standard real-time PCR method in the genome analysis. The Si NWs sensor showed high sensitivity and specificity, easy detection method, and low manufacturing cost fully compatible with standard silicon process technology. All these points are key factors for the future development of a new class of genetic point-of-care devices that are reliable, fast, low cost, and easy to use ...read more
Citations
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A Review on Biosensors and Recent Development of Nanostructured Materials-Enabled Biosensors.
V. Naresh,Nohyun Lee +1 more
TL;DR: A biosensor is an integrated receptor-transducer device, which can convert a biological response into an electrical signal as mentioned in this paper, which can transform biological signals into electrochemical, electrical, optical, gravimetric, or acoustic signals.
Journal ArticleDOI
Nanowire Electronics: From Nanoscale to Macroscale
TL;DR: A comprehensive review of the continuing efforts in exploring semiconductor nanowires for the assembly of functional nanoscale electronics and macroelectronics, including a unique design of solution-processable nanowire thin-film transistors for high-performance large-area flexible electronics.
Journal ArticleDOI
How Nanophotonic Label-Free Biosensors Can Contribute to Rapid and Massive Diagnostics of Respiratory Virus Infections: COVID-19 Case.
TL;DR: Taking the current COVID-19 pandemic as a critical case scenario, an overview of the diagnostic techniques for respiratory viruses is provided and how nanophotonic biosensors can contribute to improving such diagnostics are analyzed.
Journal ArticleDOI
Bio-acceptable 0D and 1D ZnO nanostructures for cancer diagnostics and treatment
Brandon Ortiz-Casas,Andrés Galdámez-Martínez,Jorge Gutiérrez-Flores,Andrés Baca Ibañez,Pritam Kumar Panda,Guillermo Santana,Horacio Astudillo de la Vega,Mrutyunjay Suar,Citlaly Gutiérrez Rodelo,Ajeet Kaushik,Yogendra Kumar Mishra,A. Dutt +11 more
TL;DR: The role of 0D and 1D ZnO nanostructures for diagnostics and treatment of cancer effectively and efficiently is discussed in this paper, where the authors consider captivating research advances to identify and understand fundamental properties and examine various biosensing approaches and nanomedicine aspects utilizing them.
Journal ArticleDOI
Carbon Dots as Promising Tools for Cancer Diagnosis and Therapy.
Giuseppe Nocito,Giovanna Calabrese,Stefano Forte,Salvatore Petralia,Caterina Puglisi,Michela Campolo,Emanuela Esposito,Sabrina Conoci +7 more
TL;DR: Carbon Dots (CDs) are the latest members of carbon-based nanomaterials, which since their discovery have attracted notable attention due to their chemical and mechanical properties, brilliant fluorescence, high photostability, and good biocompatibility as mentioned in this paper.
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