Journal ArticleDOI
Understanding the radical mechanism of lipoxygenases using 31P NMR spin trapping.
Luca Zoia,Raffaella Perazzini,Claudia Crestini,Dimitris S. Argyropoulos,Dimitris S. Argyropoulos +4 more
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TLDR
The quantitative (31)P NMR spin trapping methods are used, already developed for simple oxygen- and carbon-centered radicals, to understand the radical intermediates generated by enzymatic systems and more specifically lipoxygenases, to better understand the mechanism of enzymatics oxidation of linoleic acid by soybean lip oxygengenases-1.About:
This article is published in Bioorganic & Medicinal Chemistry.The article was published on 2011-05-01. It has received 19 citations till now. The article focuses on the topics: Spin trapping & Radical.read more
Citations
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Journal ArticleDOI
Lipoxygenases: From Isolation to Application
TL;DR: This review focuses on the extraction and molecular characterization of lipoxygenases in addition to the work done on the application of lip Oxygenase enzymes in the baking industry.
Journal ArticleDOI
Stable Organic Radicals in Lignin: A Review.
TL;DR: This review is focused on examining the origin of stable organic radicals in lignin, factors affecting their presence, reactions involved in their formation, and methods for their detection.
Journal ArticleDOI
Antioxidant and lipoxygenase activities of polyphenol extracts from oat brans treated with polysaccharide degrading enzymes
TL;DR: The effect of protein precipitation on soluble polyphenols is therefore dependent of the carbohydrase, as proteins with different compositions will interact differently with other molecules.
Book ChapterDOI
Structure and Characteristics of Lignin
TL;DR: In this article, a comprehensive overview of structural characteristics of lignin and related developments in the studies including experimental and computational assays, physicochemical properties investigation, isolation and separation methods, and the instrumental technologies is presented.
Journal ArticleDOI
A method for simultaneous quantification of phospholipid species by routine 31P NMR
TL;DR: The reported method, using routine (31)P NMR equipment, is suitable when fast results of a limited number of samples are requested and changing the pH of the buffer enables peak separation for different phospholipid species.
References
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Journal ArticleDOI
Leukotrienes and lipoxins: structures, biosynthesis, and biological effects
TL;DR: The multiple interaction of lipoxygenases generates compounds that can regulate specific cellular responses of importance in inflammation and immunity.
Journal ArticleDOI
Disruption of the 12/15-lipoxygenase gene diminishes atherosclerosis in apo E-deficient mice.
Tillmann Cyrus,Joseph L. Witztum,Daniel J. Rader,Rajendra K. Tangirala,Sergio Fazio,MacRae F. Linton,Colin D. Funk +6 more
TL;DR: The combined data indicate a role for 12/15-lipoxygenase in the pathogenesis of atherosclerosis and suggest that inhibition of this enzyme may decrease disease progression.
Journal ArticleDOI
Recent investigations into the lipoxygenase pathway of plants
TL;DR: The plant lipoxygenase (LOX) pathway is in many respects the equivalent of the 'arachidonic acid cascade' in animals, and hormonal and anti-fungal activities have been reported.
Journal ArticleDOI
The three-dimensional structure of an arachidonic acid 15-lipoxygenase
TL;DR: The structure of soybean lipoxygenase-1 solved to 2.6 angstrom resolution shows that the enzyme has two domains: a 146-residue beta barrel and a 693-Residue helical bundle, which appears to be a distorted octahedron in which two adjacent positions are not occupied by ligands.
Journal ArticleDOI
Crystal structure of soybean lipoxygenase L-1 at 1.4 Å resolution
Wladek Minor,Janusz Steczko,Janusz Steczko,Boguslaw Stec,Zbyszek Otwinowski,Jeffrey T. Bolin,Rick Walter,Rick Walter,Bernard Axelrod +8 more
TL;DR: The protein structure restricts access to the Fe site such that the formation of an organo-Fe intermediate seems improbable, and structural restrictions pertinent to other proposed reaction intermediates, such as planar pentadienyl and nonplanar allyl radicals, are discussed.