Journal ArticleDOI
Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein.
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TLDR
Vectors were constructed that allow foreign peptides to be expressed in Escherichia coli as fusion proteins that can be directed to the periplasm by including the leader sequence from the phoA gene on the vector.About:
This article is published in Gene.The article was published on 1988-07-15. It has received 640 citations till now. The article focuses on the topics: Maltose-binding protein & Fusion protein.read more
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Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems.
TL;DR: An overview of the most frequently used and interesting recombinant hybrids containing a polypeptide fusion partner, termed affinity tag, to facilitate the purification of the targetpolypeptides are given.
Journal ArticleDOI
Strategies for achieving high-level expression of genes in Escherichia coli.
TL;DR: Progress in the understanding of several biological processes promises to broaden the usefulness of Escherichia coli as a tool for gene expression and the remarkable increase in the availability of fusion partners offers a wide range of tools for improved protein folding, solubility, protection from proteases, yield, and secretion into the culture medium.
Journal ArticleDOI
Pax9-deficient mice lack pharyngeal pouch derivatives and teeth and exhibit craniofacial and limb abnormalities
TL;DR: Analysis shows that Pax9 is a key regulator during the development of a wide range of organ primordia during mouse embryogenesis and in Pax9-deficient embryos tooth development is arrested at the bud stage.
Journal ArticleDOI
Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element.
Shaorong Chong,Fana B. Mersha,Donald G. Comb,Melissa E. Scott,David Landry,Luis M. Vence,Francine B. Perler,Jack S. Benner,Rebecca Kucera,Christine A. Hirvonen,John J Pelletier,Henry Paulus,Ming Qun Xu +12 more
TL;DR: A novel protein purification system has been developed which enables purification of free recombinant proteins in a single chromatographic step using a modified protein splicing element from Saccharomyces cerevisiae in conjunction with a chitin-binding domain from Bacillus circulans as an affinity tag.
Journal ArticleDOI
Overcoming Expression and Purification Problems of RhoGDI Using a Family of “Parallel” Expression Vectors☆
TL;DR: The development and use of expression vectors based on three of the most frequently used gene fusion affinity tags are discussed with respect to overcoming purification problems encountered for the RhoA GDP/GTP nucleotide dissociation inhibitor (RhoGDI) and their advantages over commercially available expression vectors.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
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