Yeast sterol esters and their relationship to the growth of yeast.
R B Bailey,L W Parks +1 more
TLDR
Evidence is presented that sterol esters of ergosterol precursors are unable to be metabolized to ergosterols, and once esterified, the fatty acids do not appear to be scavenged during starvation conditions.Abstract:
Variation in the percentage of sterols esterified to long-chain fatty acids during cellular growth has been examined. Under all conditions, a constant percentage of sterol esters was maintained during exponential growth. This maintenance level was found to vary with different growth conditions. A sharp increase in the rate of esterification was observed upon entry of the culture into the stationary growth phase. The minor cellular sterol components were found to accumulate after this period of rapid sterol ester synthesis, with a relative decrease in the size of the ergosterol pool. Evidence is presented that sterol esters of ergosterol precursors are unable to be metabolized to ergosterol. Once esterified, the fatty acids do not appear to be scavenged during starvation conditions.read more
Citations
More filters
Journal ArticleDOI
Isolation and biochemical characterization of organelles from the yeast, Saccharomyces cerevisiae.
Erwin Zinser,Günther Daum +1 more
Book ChapterDOI
Regulation of glucose metabolism in growing yeast cells
TL;DR: This chapter discusses two distinct groups of yeasts—namely, glucose insensitive and glucose-sensitive yeasts, and indicates that there are possibly differences in internal metabolite concentrations, which may be expressed in the different regulatory responses.
Journal ArticleDOI
Metabolism of sterols in yeast.
TL;DR: In this article, the Metabolism of Sterols in Yeast was investigated and the results showed that it is a function of the number of chromosomes in the Yeast population.
Journal ArticleDOI
Contribution of Are1p and Are2p to steryl ester synthesis in the yeast Saccharomyces cerevisiae.
Dagmar Zweytick,Erich Leitner,Sepp D. Kohlwein,Chunjiang Yu,Jonathan Rothblatt,Günther Daum +5 more
TL;DR: In a growth competition experiment are1are2 cells grow more slowly than wild-type after several rounds of cultivation, suggesting that Are1p and Are2p or steryl esters, the product formed by these two enzymes, are more important in the natural environment than under laboratory conditions.
Book ChapterDOI
Structure and Function of Sterols in Fungi
TL;DR: The structure and function of sterols in fungi is discussed, which shows that the pythiaceous fungi do not produce sterols because they cannot carry out the epoxidation of squalene, and it is believed that Plasmodiophoromycetes, which are obligate parasites, also do not production sterols.
References
More filters
Journal ArticleDOI
A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts
TL;DR: The ability of some groups of closely related yeasts to use potassium nitrate as a source of nitrogen was applied successfully by Stelling-Dekker (1931) and later, Lodder added other nitrogen compounds, namely, ammonium sulfate, urea, asparagine, and peptone, in diagnostic tests for her classification of the nonsporogenous, nonfilamentous yeasts.
Journal ArticleDOI
Studies on the sterols and sterol esters of the intracellular organelles of maize shoots
R. J. Kemp,E. I. Mercer +1 more
TL;DR: Cholesterol is the major esterified 4-demethyl sterol of the nuclear and chloroplastidic fractions, but only the nuclear fraction has an appreciable proportion of unesterified cholesterol.
Journal ArticleDOI
Changes in the levels and composition of the esterified and unesterified sterols of maize seedlings during germination
R.J. Kemp,L.J. Goad,E.I. Mercer +2 more
TL;DR: During the germination period it was found that the level of “fast-reacting” sterol esters remains constant in all four morphological units and the composition of the shoot, scutellum and endosperm sterol mixtures does not change.
Journal ArticleDOI
The exchange of unesterified cholesterol between human low-density lipoproteins and rat erythrocyte 'ghosts'.
K. R. Bruckdorfer,C. Green +1 more
TL;DR: It is proposed that hydrophobic bonding is of great importance in maintaining the structure of ;ghosts' and lipoproteins in the presence of a wide range of chemical compounds.
Journal ArticleDOI
The sterol esters of maize seedlings.
R. J. Kemp,E. I. Mercer +1 more
TL;DR: The composition of the sterol ester fraction of the shoot, root, scutellum and endosperm of 10-day-old maize seedlings was investigated and there is a greater proportion of cholesteryl ester in the shoot than in the other tissues of the seedling.
Related Papers (5)
Metabolic interconversion of free sterols and steryl esters in Saccharomyces cerevisiae.
F R Taylor,L W Parks +1 more