Isolation of lipid particles from baker's yeast.
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TLDR
The isolation and subsequent chemical analysis of lipid particles from baker’s yeast show that triacylglycerols and sterol esters are the major components of the lipid and account for 90-95% of the total particle content.About:
This article is published in FEBS Letters.The article was published on 1974-07-15 and is currently open access. It has received 95 citations till now. The article focuses on the topics: Saccharomyces & Yeast.read more
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Phospholipid synthesis and lipid composition of subcellular membranes in the unicellular eukaryote Saccharomyces cerevisiae.
Erwin Zinser,C. D. M. Sperka-Gottlieb,E.-V. Fasch,Sepp D. Kohlwein,Fritz Paltauf,Günther Daum +5 more
TL;DR: Subcellular membranes of Saccharomyces cerevisiae, including mitochondria, microsomes, plasma membranes, secretory vesicles, vacuoles, nuclear membranes, peroxisomes, and lipid particles, were isolated by improved procedures and analyzed for their lipid composition and their capacity to synthesize phospholipids and to catalyze sterol delta 24-methylation.
Journal ArticleDOI
Storage Lipid Synthesis Is Non-essential in Yeast
Line Sandager,Maria H. Gustavsson,Ulf Stahl,Anders Dahlqvist,Eva Wiberg,Antoni Banas,Marit Lenman,Hans Ronne,Sten Stymne +8 more
TL;DR: It is concluded that neither storage lipids nor lipid bodies are essential for growth in yeast.
Journal ArticleDOI
Surface structure and properties of plant seed oil bodies
Jason T.C. Tzen,A. H. C. Huang +1 more
TL;DR: It is concluded that both oleosins and PL are required to stabilize the oil bodies and that oleOSins prevent oil bodies from coalescing by providing steric hindrance.
Journal ArticleDOI
Isolation and biochemical characterization of organelles from the yeast, Saccharomyces cerevisiae.
Erwin Zinser,Günther Daum +1 more
Journal ArticleDOI
Intracellular lipid particles of eukaryotic cells
TL;DR: Characterization of intracellular lipid particles of three different eukaryotic species, namely mammalian cells, plants and yeast are described and models for the biogenesis of lipid particles are presented and compared among the different species.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
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[A new determination of the neutral fats in blood serum and tissue. I. Principles, procedure, and discussion of the method]
M. Eggstein,F. H. Kreutz +1 more
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Subcellular localization of the prostaglandin system in the rabbit renal papilla.
TL;DR: The results indicate that in the rabbit renal papilla prostaglandin E2 is formed mainly from locally available arachidonate in membranes which sediment in the microsomal fraction and which may be derived from either cell membranes or membranes of the endoplasmic reticulum.