Journal ArticleDOI
Zinc-induced cell death in rice (Oryza sativa L.) roots
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TLDR
Results suggest that the ROS, protein phosphatase and PI-3K may function in the Zn-induced cellular toxicity in rice roots.Abstract:
Cell death in rice roots due to zinc (Zn) toxicity was investigated using inhibitors of signal molecules known to regulate programmed cell death in plants. Zn (5.0– 25.0 mM) induced cell death in a dose- and time-dependent manner. Sodium benzoate, a scavenger of reactive oxygen species (ROS), increased the cell viability under toxic Zn level (25.0 mM), suggesting a role of ROS in Zn-induced cell death. The protective role of rotenone in cell death indicated the involvement of mitochondrial electron transport chain in this Zn-induced ROS generation. Cantharidin and endothall, two serine/threonine phosphatase inhibitors, and sodium orthovanadate (Na3VO4) and phenylarsine oxide (PAO), two protein tyrosine phosphatase inhibitors, blocked Zn-induced root cell death. Conversely, K252-a, a serine/threonine kinase inhibitor, increased Zn-induced cell death. Furthermore, the phosphatidylinositol 3-Kinase (PI-3K) inhibitors, LY 294002 and wortmannin inhibited Zn-induced root cell death. These results suggest that the ROS, protein phosphatase and PI-3K may function in the Zn-induced cellular toxicity in rice roots.read more
Citations
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Journal ArticleDOI
The effect of excess Zn on mineral nutrition and antioxidative response in rapeseed seedlings.
TL;DR: It is suggested that excess Zn exerts its toxicity partially through disturbing nutrient balance and inducing oxidative stress in plants through the adaptive mechanism in Zn non-hyperaccumulator plants.
Journal ArticleDOI
An overview of heavy metal challenge in plants: from roots to shoots
TL;DR: Understanding the molecular basis of heavy metal tolerance in plants will facilitate the development of new strategies to create metal-tolerant crops, biofortified foods and plants suitable for the phytoremediation of contaminated sites.
Journal ArticleDOI
Aluminium induced oxidative stress and DNA damage in root cells of Allium cepa L.
TL;DR: Evidence was provided that Al comparable to biotic stress induced oxidative burst at the cell surface through up- or down-regulation of some of the key enzymes of oxidative metabolism ultimately resulting in oxidative stress leading to DNA damage and cell death in root cells of A. cepa.
Journal ArticleDOI
Zinc in soils, water and food crops
TL;DR: The relevant aspects of the role of Zn in the soil-water-plant agro biological system are reviewed and discussed to elucidate the strategies potentially help combating Zn deficiency problems in soil-plant-human continuum.
Journal ArticleDOI
Zinc nutrition in rice production systems: a review
Hafeez ur Rehman,Tariq Aziz,Tariq Aziz,Muhammad Farooq,Muhammad Farooq,Muhammad Farooq,Abdul Wakeel,Zed Rengel +7 more
TL;DR: Although soil application of inorganic Zn fertilizers is widely used, organic and chelated sources are better from economic and environmental perspectives and use of other methods of Zn application may improve Zn-use efficiency in rice.
References
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Journal ArticleDOI
The biochemical basis of zinc physiology
TL;DR: Majors topics addressed in this review on zinc physiology are chemistry and biochemistry; interface of biochemistry and physiology of zinc; physiology and cell and molecular biology; and pathology.
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Tansley Review No. 111: Possible roles of zinc in protecting plant cells from damage by reactive oxygen species.
TL;DR: Zinc plays critical roles in the defence system of cells against ROS, and thus represents an excellent protective agent against the oxidation of several vital cell components such as membrane lipids and proteins, chlorophyll, SH-containing enzymes and DNA.
Journal ArticleDOI
Metal-induced oxidative stress and signal transduction.
TL;DR: This review covers recent advances in metal-induced generation of reactive oxygen species; the receptors, kinases, and nuclear transcription factors affected by metals andMetal-induced oxidative stress; and global cellular phenomena associated with metal- induced ROS production and gene expression.
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Toxicity of Zinc and Copper to Brassica Species: Implications for Phytoremediation
Stephen D. Ebbs,Leon V. Kochian +1 more
TL;DR: Heavy metal-induced inhibition of Fe and Mn accumulation may have been a significant factor in reducing plant growth and the extent of Zn and Cu removal was reduced in the presence of both metals, as compared to the single heavy metal treatments.
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An improved method for monitoring cell death in cell suspension and leaf disc assays using evans blue
C. Jacyn Baker,Norton M. Mock +1 more
TL;DR: This work has used Evans blue stain to develop a spectrophotometric procedure that allows rapid, reproducible quantification of the stain retained by dead cells, and was used to compare plant/bacteria interactions involving either soybean/Pseudomonas syringae pv.