Showing papers on "Halomonas published in 2010"
TL;DR: Phylogenetic studies on haloarchaeal enzymes able to polymerize the components of PHAs reveal a novel cluster, with a close relationship with PHA polymerases of bacteria and archaea found in marine-related niches, more closely affiliated to synthases of Proteobacteria.
Abstract: Biodegradable materials with plastic or elastomeric properties are in great demand for a variety of applications. Polyhydroxyalkanoates (PHAs), polyesters synthesized by microorganisms, possess such desired features. Industrial production of PHAs is currently achieved using recombinant Escherichia coli. Nevertheless, recent research on halophiles, salt requiring microorganisms, has shown a remarkable potential for biotechnological production of PHAs. The halophilic archaeon Haloferax mediterranei accumulates a co-polymer, i.e., poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in large amounts using glucose, starch, and hydrolyzed whey as carbon sources. Chemical composition and molecular weight of PHAs produced by H. mediterranei can be modified depending on the substrate utilized as precursor. Phylogenetic studies on haloarchaeal enzymes able to polymerize the components of PHAs (i.e., PHA synthases) reveal a novel cluster, with a close relationship with PHA polymerases of bacteria and archaea found in marine-related niches. On the other hand, sequences of PHA synthases of two halophilic bacteria are more closely affiliated to synthases of Proteobacteria. Several bacterial species of the family Halomonadaceae accumulate PHAs. Halomonas boliviensis reached PHA yields and volumetric productivities close to the highest reported so far. Furthermore, H. boliviensis and other Halomonas species are able to co-produce PHA and osmolytes, i.e., ectoines and hydroxyectoine, in one process.
245 citations
TL;DR: Production of a biodegradable copolymer, PHB-co-PHV is described by a moderately haloalkalitolerant Halomonas campisalis, isolated from Lonar Lake, India, which could be achieved by providing simple carbon source viz. maltose.
100 citations
TL;DR: This work tried to isolate waste glycerol utilizing bacteria, and obtained the alkalo- and halophile bacteria Halomonas sp.
Abstract: Biodiesel fuel is favored as a type of carbon neutral energy. To popularize its usage, by-product waste glycerol utilization is a critical problem. We tried to isolate waste glycerol utilizing bacteria, and obtained the alkalo- and halophile bacteria Halomonas sp. KM-1. This strain produced bioplastic poly(3-hydroxybutyrate) (PHB) in a simple medium and diluted waste glycerol as a sole carbon source.
90 citations
TL;DR: On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain BH1(T) is considered to represent a novel species, for which the name Halomonas titanicae sp.
Abstract: A Gram-negative, heterotrophic, aerobic, non-endospore-forming, peritrichously flagellated and motile bacterial strain, designated BH1T, was isolated from samples of rusticles, which are formed in part by a consortium of micro-organisms, collected from the RMS Titanic wreck site. The strain grew optimally at 30–37 °C, pH 7.0–7.5 and in the presence of 2–8 % (w/v) NaCl. We carried out a polyphasic taxonomic study in order to characterize the strain in detail. Phylogenetic analyses based on 16S rRNA gene sequence comparison indicated that strain BH1T clustered within the branch consisting of species of Halomonas. The most closely related type strains were Halomonas neptunia (98.6 % 16S rRNA sequence similarity), Halomonas variabilis (98.4 %), Halomonas boliviensis (98.3 %) and Halomonas sulfidaeris (97.5 %). Other closely related species were Halomonas alkaliphila (96.5 % sequence similarity), Halomonas hydrothermalis (96.3 %), Halomonas gomseomensis (96.3 %), Halomonas venusta (96.3 %) and Halomonas meridiana (96.2 %). The major fatty acids of strain BH1T were C18 : 1 ω7c (36.3 %), C16 : 0 (18.4 %) and C19 : 0 cyclo ω8c (17.9 %). The DNA G+C content was 60.0 mol% (T m). Ubiquinone 9 (Q-9) was the major lipoquinone. The phenotypic features, fatty acid profile and DNA G+C content further supported the placement of strain BH1T in the genus Halomonas. DNA–DNA hybridization values between strain BH1T and H. neptunia CECT 5815T, H. variabilis DSM 3051T, H. boliviensis DSM 15516T and H. sulfidaeris CECT 5817T were 19, 17, 30 and 29 %, respectively, supporting the differential taxonomic status of BH1T. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain BH1T is considered to represent a novel species, for which the name Halomonas titanicae sp. nov. is proposed. The type strain is BH1T (=ATCC BAA-1257T =CECT 7585T =JCM 16411T =LMG 25388T).
73 citations
TL;DR: It was concluded that H. strobilaceum could be a valuable halophyte for phytoremediation of oil-polluted hypersaline environments via rhizosphere technology.
64 citations
TL;DR: This study showed that the solar saltern of Sfax is an optimal environment for halophilic bacterial growth, where diverse viable bacterial communities are available and may have many industrial applications.
Abstract: Bacterial screenings from solar saltern in Sfax (Tunisia) lead to the isolation of 40 moderately halophilic bacteria which were able to grow optimally in media with 5-15% of salt. These isolates were phylogenetically characterized using 16S rRNA gene sequencing. Two groups were identified including 36 strains of Gamma-Proteobacteria (90%) and 4 strains of Firmicutes (10%). The Gamma-Proteobacteria group consisted of several subgroups of the Halomonadaceae (52.5%), the Vibrionaceae (15%), the Alteromonadaceae (10%), the Idiomarinaceae (7.5%), and the Alcanivoracaceae (5%). Moreover, three novel species: 183ZD08, 191ZA02, and 191ZA09 were found, show <97% sequence similarity of the 16S rRNA sequences while compared to previously published cultivated species. Most of these strains (70%) were able to produce hydrolases: amylases, proteases, phosphatases, and DNAases. Over the isolates, 60% produced phosphatases, 15.0% proteases, 12.5% amylases and DNAases equally. This study showed that the solar saltern of Sfax is an optimal environment for halophilic bacterial growth, where diverse viable bacterial communities are available and may have many industrial applications.
56 citations
TL;DR: There is excellent agreement between the phylogenies based on the two rRNA gene sequences, but the 23S r RNA gene showed higher resolution in the differentiation of species of the family Halomonadaceae.
Abstract: A phylogenetic study of the family Halomonadaceae was carried out based on complete 16S rRNA and 23S rRNA gene sequences. Several 16S rRNA genes of type strains were resequenced, and 28 new sequences of the 23S rRNA gene were obtained. Currently, the family includes nine genera (Carnimonas, Chromohalobacter, Cobetia, Halomonas, Halotalea, Kushneria, Modicisalibacter, Salinicola and Zymobacter). These genera are phylogenetically coherent except Halomonas, which is polyphyletic. This genus comprises two clearly distinguished clusters: group 1 includes Halomonas elongata (the type species) and the species Halomonas eurihalina, H. caseinilytica, H. halmophila, H. sabkhae, H. almeriensis, H. halophila, H. salina, H. organivorans, H. koreensis, H. maura and H. nitroreducens. Group 2 comprises the species Halomonas aquamarina, H. meridiana, H. axialensis, H. magadiensis, H. hydrothermalis, H. alkaliphila, H. venusta, H. boliviensis, H. neptunia, H. variabilis, H. sulfidaeris, H. subterranea, H. janggokensis, H. gomseomensis, H. arcis and H. subglaciescola. Halomonas salaria forms a cluster with Chromohalobacter salarius and the recently described genus Salinicola, and their taxonomic affiliation requires further study. More than 20 Halomonas species are phylogenetically not within the core constituted by the Halomonas sensu stricto cluster (group 1) or group 2 and, since their positions on the different phylogenetic trees are not stable, they cannot be recognized as additional groups either. In general, there is excellent agreement between the phylogenies based on the two rRNA gene sequences, but the 23S rRNA gene showed higher resolution in the differentiation of species of the family Halomonadaceae.
54 citations
TL;DR: Two halotolerant and alkaliphilic bacteria, designated as Halomonas sp.
47 citations
TL;DR: Although phenotypic similarity does not always reflect phylogenetic relatedness, significant congruence between both features in Halomonas is found, making it clear that denitrifying ability should be considered as an important phenotypesic and phylogenetic discriminatory marker within this genus.
43 citations
TL;DR: On the basis of data from this polyphasic study, strain TRM 0175(T), isolated from a soil sample taken from a salt lake in Xinjiang Province, north-west China, represents a novel species of the genus Halomonas, for which the name Halomon as xinjiangensis sp.
Abstract: A novel bacterium, TRM 0175(T), belonging to the genus Halomonas, was isolated from a soil sample taken from a salt lake in Xinjiang Province, north-west China. The isolate was Gram-negative, aerobic, rod-shaped and motile by means of peritrichous flagella. It was catalase-positive and oxidase-negative. Growth occurred at NaCl concentrations of 0-20 % (optimum at 10-13 %), at 15-50 degrees C (optimum at 37 degrees C) and at pH 6.0-9.0 (optimum at pH 7.0). Metabolism was respiratory with oxygen as terminal electron acceptor. Acid was produced from D-ribose, D- and L-arabinose, D-xylose, D-galactose, D-mannose, L-rhamnose, cellobiose, maltose, trehalose and D- and L-fucose and was produced weakly from aesculin. The predominant ubiquinone was Q-9. The major fatty acids were C(18 : 1)omega7c and C(19 : 0) cyclo omega8c. The G+C content of the genomic DNA was 60.0 mol%. The affiliation of strain TRM 0175(T) with the genus Halomonas was confirmed by 16S rRNA gene sequence comparisons. The most closely related species was Halomonas anticariensis; 16S rRNA gene sequence similarity between H. anticariensis FP35(T) and strain TRM 0175(T) was 95.3 %. Phenotypically, some characteristics of TRM 0175(T) differed from those of H. anticariensis. On the basis of data from this polyphasic study, strain TRM 0175(T) represents a novel species of the genus Halomonas, for which the name Halomonas xinjiangensis sp. nov. is proposed; the type strain is TRM 0175(T) (=CCTCC AB 208329(T) =KCTC 22608(T)).
43 citations
TL;DR: Combined phenotypic and DNA-DNA hybridization data support the conclusion that the 14 Halomonas strains isolated from the blood of two patients and from dialysis machines of a renal care centre represent three novel species of the genusHalomonas.
Abstract: A total of 14 Halomonas strains were isolated from the blood of two patients and from dialysis machines of a renal care centre. The strains were Gram-negative, halophilic, motile and non-spore-forming rods. They produced cream-coloured colonies and contained Q-9 as the predominant ubiquinone and C(18 : 1)omega7c and C(16 : 0) as the major fatty acids. Phylogenetic analysis based on 16S rRNA gene sequencing showed that the 14 isolates were most closely related to Halomonas magadiensis 21 MI(T) with 98.1-98.9 % sequence similarity and that they formed three separate lineages among themselves. Combined phenotypic and DNA-DNA hybridization data support the conclusion that they represent three novel species of the genus Halomonas, for which the names Halomonas stevensii sp. nov. (type strain S18214(T)=KCTC 22148(T)=DSM 21198(T)), Halomonas hamiltonii sp. nov. (type strain W1025(T)=KCTC 22154(T)=DSM 21196(T)) and Halomonas johnsoniae sp. nov. (type strain T68687(T)=KCTC 22157(T)=DSM 21197(T)) are proposed.
TL;DR: The results suggest that the moderately halophilic bacteria may be developed as promising sources for the discovery of novel bioactive substances.
Abstract: A total of 45 moderately halophilic bacteria was isolated from sediment and saline water collected from the Weihai Solar Saltern (China). The phylogenetic position of all the isolated strains was determined by 16S rRNA sequencing. The halophilic strains were tested for their antimicrobial activity. Cytotoxicity assay was performed to determine which of the halophilic strains could inhibit proliferation of human hepatocellular carcinoma Bel 7402 cells. Our results showed that all of the isolated 45 strains displayed moderately halophilic characteristics. Phylogenetic analysis indicated that 17 of the isolated strains were related to the phylum Firmicutes and belonged to four genera, Bacillus, Halobacillus, Planococcus and Salinicoccus. The other strains identified as genus of Halomonas belonged to phylum gamma-Proteobacteria. Most of the halophilic bacterial strains showed potent activities against Gram-positive bacteria, human pathogenic fungi and plant pathogenic fungi. In addition, the crude extracts from 14 halophilic bacterial strains showed cytotoxic activity against tumor cells Bel 7402, and five of them showed remarkable activities with IC(50) less than 40 mu g ml(-1). Our results suggest that the moderately halophilic bacteria may be developed as promising sources for the discovery of novel bioactive substances.
TL;DR: In this study, Pseudomonas, Bacillus and Brevibacterium strains were the most efficient hydrocarbon-degrading marine bacteria and Halomonas strains produced the highest amount of efficient biopolymers with emulsifying activity.
TL;DR: Investigation of the cultivable and non-cultivable members of the microbial community present on the stonework of the medieval Chapel of St. Virgil reveals the presence of highly specialized microorganisms that were able to thrive and survive after several desalination and disinfection treatments in the extreme environment presented by the salt-attacked Chapel.
Abstract: In the last few years several investigations, based on culture-dependent and -independent techniques, have shown that salt-attacked stone surfaces present a habitat for extremely salt tolerant and moderate halophilic microorganisms. The inner walls of the Chapel of St. Virgil in Vienna (Austria) are an example of this phenomenon. Salt crusts cover most of the wall surfaces and salt crystallization in the porous space of the stone is causing decohesion of material and destruction of the original medieval paintings. The salt, together with the oligotrophic conditions, creates a very special and extreme habitat for halotolerant and halophilic microorganisms.In this study we investigate and monitor the cultivable and non-cultivable members of the microbial community present on the stonework of the medieval Chapel of St. Virgil after several severe disturbances of the microbial environment caused by desalination and disinfection treatments. With this finality, a combination of culture-dependent and -independent techniques was selected. The genetic diversity of a total of 104 bacterial strains isolated from the stone samples was analysed by denaturing gradient gel electrophoresis (DGGE), random amplified polymorphic DNA (RAPD) analysis and 16S rRNA gene sequencing. Strains were distributed over 29 groups on the basis of their RAPD patterns. Only 19 groups were differentiated by DGGE. Comparative sequence analyses showed that the isolated strains belong to related species of the genera Halobacillus (47.1%), Bacillus (35.6%), Acinetobacter (4.8%), Halomonas (3.9%), Nesterenkonia (2.9%), Paucisalibacillus (2.9%), Paenibacillus (1%), Staphylococcus (1%) and Exiguobacterium (1%).In addition, polymerase chain reaction DGGE fingerprints, in combination with the creation of clone libraries and sequencing analyses, were used to monitor and identify Archaea, the non-cultivable fraction of the microbial community. The detected archaeal sequences were closely related to different uncultured archaeons as well as to the cultured genera Halococcus and Halalkalicoccus and Halobacterium.Cultivation and molecular analyses revealed the presence of highly specialized microorganisms that were able to thrive and survive after several desalination and disinfection treatments in the extreme environment presented by the salt-attacked Chapel of St. Virgil.
TL;DR: Strain LC6(T) was aerobic, heterotrophic, and able to utilize various carbohydrates and other substrates as carbon source, and resembled recognized Halomonas species with respect to various physiological, biochemical and nutritional characteristics.
Abstract: A moderately halophilic, motile, Gram-negative, rod-shaped bacterium, strain LC6(T), was isolated from a water sample of lake Laguna Colorada in the Bolivian Andes. The major cellular fatty acids were C(18 : 1)omega7c, iso-C(16 : 1)omega7c 2-OH, C(16 : 0) and C(12 : 0) 3-OH. The respiratory ubiquinones found in strain LC6(T) were Q-9 (97 %) and Q-8 (3 %). Strain LC6(T) was aerobic, heterotrophic, and able to utilize various carbohydrates and other substrates as carbon source. The G+C content of the genomic DNA of strain LC6(T) was 52.5 mol%. The organism was able to grow at pH 6.0-11.0 (optimum, pH 7.0-8.0), at 4-45 degrees C (optimum, 30-35 degrees C) and in the presence of 0.5-20 % (w/v) NaCl (optimum, 1-3 %, w/v). Based on 16S rRNA gene sequence analysis, strain LC6(T) was most closely related to Halomonas hydrothermalis DSM 15725(T) and Halomonas venusta DSM 4743(T) (98.8 % similarity), followed by Halomonas aquamarina DSM 30161(T), Halomonas axialensis DSM 15723(T) and Halomonas meridiana DSM 5425(T) (98.4 %). However, levels of DNA-DNA relatedness between strain LC6(T) and the above type strains were low (<31 %). Strain LC6(T) resembled recognized Halomonas species with respect to various physiological, biochemical and nutritional characteristics. Combined phenotypic data and DNA-DNA hybridization data supported the conclusion that strain LC6(T) represents a novel species of the genus Halomonas, for which the name Halomonas andesensis is proposed. The type strain is LC6(T) (=CCUG 54844(T)=LMG 24243(T)=DSM 19434(T)).
Patent•
25 Feb 2010
TL;DR: In this article, the alpha-amylase enzyme obtained from Halomonas variabilis WDG195 was described and compared with an α-AMylase obtained from the same species.
Abstract: Compositions and methods relating to an alpha-amylase enzyme obtained from Halomonas variabilis WDG195 are described.
TL;DR: The strains of Halomonas, Clostridium and especially Bacillus might be the key producers of xylanase and CMCase in the community and the worldwide problem of black liquor treatment and renewable resource utilization would benefit from these microorganisms in application potentials.
TL;DR: Preliminary experiments suggested that syntrophic associations between strains of the Pseudoalteromonas and Halomonas genera explain the dynamics of polyhydroxyalkanoate accumulation in some microbial mats.
TL;DR: In this article, two agarolytic bacterial species, Halomonas aquamarina and Alteromonas macleodii, were found to be halophilic (optimum 2.5% NaCl, w/v), slightly alkaliphilic and grew best at 25°C.
TL;DR: The complete core LPS structure from this bacterium was hydrolyzed both under mild acid and strong alkaline conditions and it was suggested that the branched heptose was not stoichiometrically substituted.
Abstract: Halophilic and halotolerant Gram-negative bacteria are microorganisms which thrive in high salt environments. LPS are the major components of their outer leaflet, nevertheless very little is known about the role of this molecules in the adaptation mechanisms of extremophiles. Recently we determined the O-chain repeating unit structure of the LPS from Halomonas alkaliantarctica strain CRSS, an haloalkaliphilic Gram-negative bacterium isolated from salt sediments of a saline lake in Cape Russell in Antarctic continent. The polysaccharide is constituted of the trisaccharidic repeating unit: →3)-β-l-Rhap-(1→4)-α-l-Rhap-(1→3)-α-l-Rhap-(1→. In this paper we report the complete core LPS structure from this bacterium. The LPS was hydrolyzed both under mild acid and strong alkaline conditions. The MALDI spectra showed the presence of two glycoforms. The most abundant was recovered after HPAEC purification of the alkaline hydrolyzed product and was characterized by means of 2D-NMR spectroscopy. A comparison of the MALDI-PSD spectra of the two glycoforms suggested that the branched heptose was not stoichiometrically substituted.
Patent•
22 Sep 2010
TL;DR: Halomonas Xianhensis A-1CGMCC No 2941 is a new strain of the halomonas, is separated from a high-salinity environment polluted by petroleum, can degrade various kinds of polyaromatic hydrocarbon such as phenanthrene, anthracene or fluoranthene and the like, and uses the phenanthene, the anthracenes or the fluoranthen as unique carbon source for growth as discussed by the authors.
Abstract: The invention discloses halomonas capable of degrading polyaromatic hydrocarbon and application thereof. Halomonas Xianhensis A-1CGMCC No 2941 is a new strain of the halomonas, is separated from a high-salinity environment polluted by petroleum, can degrade various kinds of polyaromatic hydrocarbon such as phenanthrene, anthracene or fluoranthene and the like, and uses the phenanthrene, the anthracene or the fluoranthene as unique carbon source for growth. The halomonas can completely degrade 100mg/L phenanthrene within 10 days in a liquid medium with 5 percent of salinity and 0.5 percent of yeast powder, and has 10mg/ (L.d) of average degradation rate; and meanwhile, the stain can grow in the environment with 27.5 percent of high salinity and wider salinity rang (0.05 to 27.5 percent), belongs to a moderate halophilic bacterium class, has unique advantages of controlling pollution of the polyaromatic hydrocarbon in salt environment, and is particularly suitable for high-salinity environment and environment with much salinity variation. The halomonas xianhensis A-1CGMCC No 2941 can be used for degrading the polyaromatic hydrocarbon.
Journal Article•
TL;DR: The results show that the halophilic bacteria population diversity is abundant in enriched culture of sedimentary soil from Daban Salt Lake in Xinjiang.
Abstract: The genomic DNA isolated from enriched culture of sedimentary soil samples from Daban Salt Lake in Xinjiang was used as PCR template to amplify 16S rRNA gene and the library carrying 16S rRNA genes of halophilic bacteria was constructed,100 clones of the library were selected randomly and sequenced for molecular systematic analysis.Phylogenetic analysis indicated that 100 selected clones were clustered into twenty-seven species of nine genera,a lineage of the domain bacteria.Among them,Bacillus was the most dominant genus as represents 48% of the clones,followed by Halobacillus(14%) and Halomonas(13%).Furthermore,ten clones may be potential novel species or genera.The results show that the halophilic bacteria population diversity is abundant in enriched culture of sedimentary soil from Daban Salt Lake in Xinjiang.
Patent•
10 May 2010
TL;DR: In this article, a consortium of the following bacteria (Rhodococcus sp.SMB37, Rhodococcus and Arthrobacterium sp., Thalassospira sp., Halomonas sp., Microbacterium and Salalinicola socius) were used to destroy polycyclic carbohydrates under conditions of high salt concentrations (NaCl to 7%).
Abstract: FIELD: biotechnologies. ^ SUBSTANCE: agent represents a consortium of the following bacteria - Rhodococcus sp.SMB37, Rhodococcus sp.SMB38, Arthrobacter sp.SMB32, Microbacterium sp.SMB33, Thalassospira sp.SMB34, Halomonas sp.SMB31 and "Salinicola socius" SMB35, deposited in collection of Chemical mutagenesis laboratory of Institute of Ecology and Genetics of Microorganisms, Urals department of Russian Academy of Sciences under No. SMB3. ^ EFFECT: high speed of polycyclic carbohydrates destruction under conditions of high salt concentrations (NaCl to 7%). ^ 2 tbl
Journal Article•
TL;DR: The research results indicated that there was not only abundant phylogenetic diversity of moderately halophilic bacteria, but also some unknown bacteria groups existed in Suaeda salsa L.
Abstract: The traditional isolation and identification method and analysis based on 16S rRNA sequences were used to investigate the phylogenetic diversity of endophytic moderately halophilic bacteria of Suaeda salsa L.in Dongying.According to physiological and biochemical characteristics,16S rRNA sequences and phylogenetic analysis,15 isolated endophytic bacterial strains were classified into four groups,belonging to genera Chromohalobacter,Kushneria,Halomonas or Bacillus separately.16S rRNA sequences of 4 strains in Group I had the highest similarity to Chromohalobacter israelensis(95%).Group II which contained 7 strains of the genus Kushneria is the dominant group in Suaeda salsa L..The strains in Group Ⅲ bear the closest affiliation to Haererehalobacter sp.JG 11(99%),which is not classified definitely.The only en-dospore-forming bacterium was classified into group IV.Its 16S rRNA sequence similarities with respect to the known Bacillus species were ≤ 96%,which was presumed to be potential novel species.Among these isolated strains,3 strains produced proteinase;14 strains produced esterase;8 strains produced DNase;11 strains produced galactosidase;14 strains produced urease.The research results indicated that there was not only abundant phylogenetic diversity of moderately halophilic bacteria,but also some unknown bacteria groups existed in Suaeda salsa L..
Journal Article•
TL;DR: NY-11 could import extracellular ectoine and glycine betaine to improve osmotolerance, and the synthesis of protein was obviously depressed by the increased NaCl concentration in medium, while the components of the intracellular compatible solutes varied with the changing of NaCl concentrations.
Abstract: A moderately halophilic bacterium Halomonas sp.NY-11 was isolated from our laboratory,and its osmotolerance property and mechanism were studied.Strain NY-11 could maximally tolerate 260 g/L NaCl in LB medium and its tolerance to NaCl was over 150 g/L in M63.The absence of chloridion would obviously inhibit the growth of NY-11 under salt and potassium was more suitable to provide intracellular osmotic pressure than lithium and magnesium.NY-11 accumulated potassium,free amino acids and other compatible solutes to equilibrate extracellulary osmotic pressure.The components of the intracellular compatible solutes of NY-11 varied with the changing of NaCl concentrations in medium,while ectoine,which was the most important component,increased with the increasing of NaCl concentration,reaching 71.5 mg/g by dry weight under 120 g/L NaCl.NY-11 could import extracellular ectoine and glycine betaine to improve osmotolerance,and the synthesis of protein was obviously depressed by the increased NaCl concentration in medium.Fig 5,Tab 2,Ref 24
Journal Article•
TL;DR: The cultivable moderately bacterial population diversity is abundant in these two mud volcanoes in Xinjiang and there are potential microbial resources in this environment, worth further studying and exploitating.
Abstract: [Objective] In order to understand the diversity of cultivable moderately halophilic bacteria from two mud volcanoes in Wusu and Dushanzi,Xinjiang.[Method]The separation of cultivable microbes based on the samples from the two mud volcanoes,detection of 16S rDNA and analysis of systematical developoment were conducted with 10% NaCl concentration.[Result] A total of twenty-six moderately halophilic bacteria and actinomyces were isolated from the two mud volcanoes in Xinjiang.Thirteen strains from Wusu mud volcano grouped into seven genera,including Nesterenkonia,Bacillus,Salinicoccus,Nocardiopsis,Halomonas,Halophilic and Micrococcacea.Thirteen strains from Dushanzi mud volcano belonged to six genera,including Halomonas,Marinobacter,Bacillus,Salinicoccus,Nocardiopsis and Halolactibacillus.Nesterenkonia was typical of the Wusu mud volcano sample,while Halomonas and Marinobacter prevailed in the Dushanzi mud volcano sample.Additionally,four poteneial novel species were found.[Conclusion] The cultivable moderately bacterial population diversity is abundant in these two mud volcanoes.In addition,there are potential microbial resources in this environment,which is worth further studying and exploitating.
Patent•
25 Feb 2010
TL;DR: In this article, the alpha-amylase enzyme obtained from Halomonas variabilis WDG195 was described and compared with an α-AMylase obtained from the same species.
Abstract: Compositions and methods relating to an alpha-amylase enzyme obtained from Halomonas variabilis WDG195 are described.
01 Jan 2010
TL;DR: Glycerol from bio-diesel production was used in the production of poly(hydrohyalkanoates) feeding cultures of the haloalkaliphilic bacterium Halomonas pantelleriensis and the thermal characteristics of polymers were obtained using a differential scanning calorimeter and the average viscositymolecular weight was assessed through specific Viscosity measurements.
Abstract: Glycerol from bio-diesel production was used in the production of poly(hydrohyalkanoates) feeding cultures of the haloalkaliphilic bacterium Halomonas pantelleriensis. The micro-organism grew well and biosynthesized poly[3-hydroxybutyrate (P3HB)]. Themaximumyields, 10-11% of cell dryweight, was obtained after 72 hwith 0.5%industrial glycerol and nitrogen source 0.1 g l-1 at 35°C and after 10 days with glycerol 1.0%. The growth with 0.1%glycerol and 0.4%valerate gave a mixed polymer P(HBHV), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in a ratio 90:10. For quantification of glycerol and of valerate during theH.pantelleriensis growth, and of PHAs, 1H -NMRwas used.MS spectroscopy, FTIR and 1H, 13C-NMR were used to chemically characterize the PHAs. The thermal characteristics of polymers were obtained using a differential scanning calorimeter and the average viscositymolecular weight was assessed through specific viscosity measurements.