Showing papers in "Journal of Biotechnology in 2010"

TL;DR: The rationale, potential and flexibility of tumor spheroid mono- and cocultures for implementation into state of the art anti-cancer therapy test platforms are highlighted and the relevance of the cancer stem cell hypothesis for cancer cure is highlighted.

TL;DR: It is concluded that selected algae species can be good substrates for biogas production and that anaerobic fermentation can seriously be considered as final step in future microalgae-based biorefinery concepts.

TL;DR: An exclusive overview of known monooxygenases is presented, based on the type of cofactor that these enzymes require, which includes not only the cytochrome P450 and flavin-dependent mono oxygengenases, but also enzymes that utilize pterin, metal ions, or no cofactor at all.

TL;DR: A special group of ionic liquids, deep eutectic solvents (DESs) have been tested as cosolvents in enzyme-catalyzed hydrolysis of a chiral (1,2)-trans-2-methylstyrene oxide.

TL;DR: The elicitation effect of SA on phenolic compound accumulations correlated with the PAL activity, but not with the TAT activity, which indicates that PAL may be the key enzyme for the biosynthesis of salvianolic acid B and caffeic acid.

TL;DR: It is demonstrated that self-assembled cell-based microtissues can be used to generate small diameter TEBV, and the significant enhancement of ECM expression and maturation, together with the pre-vascularization capacity makes this approach highly attractive in terms of generating functional small diameterTEBV devoid of any foreign material.

TL;DR: Investigating the feasibility of non-thermal atmospheric plasma treatment for cancer therapy and examining the mechanism by which plasma induces anti-proliferative properties and cell death in human colorectal cancer cells found that plasma treatment to the cells increases β-Catenin phosphorylation, suggesting that β-catenin degradation plays a role at least in part in plasma-induced anti- Proliferatives activity.

TL;DR: It is demonstrated that a microcarrier-based stirred culture system is adequate for human MSC expansion, using a low-serum containing medium, allowing the generation of significant cell numbers for potential applications in regenerative medicine.

TL;DR: Algae-based vaccination significantly reduced the pathogen load in the spleen and the intestine of treated mice and protected 80% of them against lethal doses of S. aureus, indicating that C. reinhardtii may play an important role in molecular pharming, as it combines the beneficial features of land plant vaccines, while offering unmatched ease of growth compared to other members of the plant kingdom.

TL;DR: In this article, the authors investigated the feasibility of non-thermal atmospheric plasma treatment for cancer therapy and examined the mechanism by which plasma induces anti-proliferative properties and cell death in human colorectal cancer cells.

TL;DR: It is suggested that elements of the secretory pathway, including Golgi apparatus, cytoskeleton protein binding and small GTPase-mediated signal transduction are enriched and thus may play a role in the increased recombinant protein production observed under butyrate treatment at 33 degrees C.

TL;DR: The results show, for the first time, that the use of controlled bioreactors is critical to ensure the production of high quality hESCs, and the incorporation of an automated perfusion system in the bioreactor enhanced culture performance and allowed the continuous addition of small molecules assuring higher cell concentrations for a longer time period.

TL;DR: It is demonstrated that R. opacus PD630 has the uncommon capacity to grow in defined media supplemented with glucose at a concentration of 300 g l(-1) during batch-culture fermentations, and the importance of the C/N ratio of the medium composition is described.

TL;DR: How the ratio of off- and on-switching of a fluorophore affects resolution is discussed and a strategy to customize photoswitching characteristics to achieve optimal optical resolution is presented.

TL;DR: The promoter of the thiamine biosynthesis gene P(THI11) did not only show high transcript levels at low specific growth rate, but also exhibited interesting regulatory properties dependent on the availability ofThiamine in the growth medium.

TL;DR: It is demonstrated that impaired trans-acting factors and spontaneous mutations to the DNA are not responsible for the reduced HC and LC transcription, which indicates that DNA methylation contributes to production instability and will be beneficial for generation of high-producing cell lines with stable productivity.

TL;DR: The DE-Hep showed a hepatocyte morphology with sub-organized cells and exhibited many liver-functions including transporter activity and capacity to metabolize drugs specific for important cytochrome P450 sub-families, marking an important step in differentiation of hESC into functional hepatocytes.

TL;DR: It was found that the nano-scale silver can be synthesized in reverse micelles using the glycolipid as stabilizer of nanoparticles and the silver nanoparticles synthesised in this study were uniform and stable for 2 months, therefore, the biosurfactant-mediated nanoparticles synthesis can be considered as "green" stabilizing agents.

TL;DR: This work shows an efficient immobilization method based on specific interaction between His-tagged NADH oxidase and functionalized single-walled carbon nanotubes without requiring enzyme purification for immobilization, and demonstrates good loading capacity and stability while maintaining 92% maximum activity of the native enzyme.

TL;DR: The high polymer yields and production rates suggest that enrichment of GAOs can be a fruitful strategy for mixed culture production of PHA from waste substrates.

TL;DR: Polyhydroxyalkanoates produced from fermented molasses and synthetic feeds containing single volatile fatty acids (VFAs) by an open mixed culture enriched in glycogen accumulating organisms (GAOs) were characterized with regards to molecular weight and thermal properties.

TL;DR: The results of this work provide a basis for the development of an enzymatic detoxification process for fumonisin B(1) in food and animal feed, especially under oxygen limited conditions, as they are found, e.g. in ensilaged forage or in the intestinal tract of animals.

TL;DR: In the present review, the most important concepts of far-field microscopy with subdiffraction resolution are introduced and the underlying physical concepts are discussed, and practical considerations for the application are made.

TL;DR: In conclusion, acceleration of wound closure, low cytotoxicity and irritation as well as possible prolonged morphine release make SLN an interesting approach for innovative wound management.

TL;DR: Only ionic liquid co-solvent systems with an intermediate capacity for solubilizing the proteinogenic amino acid tryptophan were found to permit effective renaturation of the model protein rPA, and the most effective refolding enhancer among the tested ionic liquids was EMIM chloride.

TL;DR: The most stable genes amongst six potential reference genes in 34 samples from diverse cultivation conditions were evaluated, and sar1 encoding for a small GTPase was found to be the most stable gene, whereas act encoding for actin was not amongst the best validated ones.

TL;DR: It is concluded that impedance spectroscopy is an appropriate method for non-invasive characterisation of osteogenic differentiation of hMSCs, which is relevant for quality control of cell-based implants and cell- based test systems for drug development.

TL;DR: The results confirm the contribution of this particular GmGSTU4 isoenzyme from soybean in the detoxification of fluorodifen and alachlor, and provide the basis towards the development of transgenic plants with improved phytoremediation capabilities for future use in environmental cleanup of herbicides.

TL;DR: A liquid chromatography-mass spectrometry based metabolomics platform is established to identify extracellular metabolites in the medium of recombinant Chinese hamster ovary fed-batch reactor cultures, and malate accumulation was the most significant.