Showing papers on "Reagent published in 1983"
TL;DR: Using a refined two-stage gold amalgamation preconcentration technique in conjunction with cold vapor atomic absorption (CVAA) detection, mercury in various Pacific Northwest coastal waters has been determined through the use of extremely clean reagents and special handling techniques, the blank has been reduced and reproducibility greatly improved for both reducible mercury and total mercury as discussed by the authors.
527 citations
TL;DR: A rapid and precise microfluorometric method for the determination of free fatty acid concentrations in 2-5 microliters of plasma, which corrects the previously reported underestimate of plasma free fatty acids with enzymic methods.
363 citations
345 citations
TL;DR: A synthesis of the N-hydroxysuccinimide ester of S-acetylthioacetic acid is described, which has advantages over the currently available reagents used to introduce sulfhydryl groups into a variety of proteins.
289 citations
Patent•
25 Jan 1983
TL;DR: An automatic analyzing apparatus for effecting chemical analyses for various sample liquids such as blood, urine, and the like, comprising a sample delivery pump for metering a sample liquid into a reaction cuvette, a reagent delivery pump, and circuitry for receiving the photometric results and selecting therefrom given quantitative analytical data of a given test item.
Abstract: An automatic analyzing apparatus for effecting chemical analyses for various sample liquids such as blood, urine, and the like, comprising a sample delivery pump for metering a sample liquid into a reaction cuvette, a reagent delivery pump for delivering to the reaction cuvette a given amount of a given reagent selected from a plurality of reagents contained in a reagent cassette, to form a test liquid, a feed mechanism for successively supplying reaction cuvettes along a circular reaction line, a plurality of photometering sections arranged along the reaction line for effecting a plurality of photometric and/or nephelometric and/or fluorometric measurements for each test liquid at different time instances to produce a plurality of photometric results, and circuitry for receiving the photometric results and selecting therefrom given quantitative analytical data of a given test item.
273 citations
263 citations
223 citations
TL;DR: Bis(pentamethylcyclopentadienyl)lutetium complexes Lu(η5-C5Me5)2R (R = Me, H) react at 20-50 °C in hydrocarbon solvents with benzene, pyridine, the ylide CH2PPh3, and tetramethylsilane to give stable, isolatable products of C-H activation.
Abstract: Bis(pentamethylcyclopentadienyl)lutetium complexes Lu(η5-C5Me5)2R (R = Me, H) react at 20–50 °C in hydrocarbon solvents with benzene, pyridine, the ylide CH2PPh3, and tetramethylsilane to give stable, isolatable products of C–H activation, i.e. metallation at a carbon of the reagent with extrusion of R–H.
174 citations
TL;DR: In this article, anionic activation of Si-H, Si-O and Si-N bonds by fluoride ions under heterogeneous conditions is reported, which constitutes an extension of the Michael reaction since the addition occurs even with crowded ketones.
166 citations
TL;DR: Micromolar concentrations of mercury, silver, and other reagents known to react with sulfhydryl groups are shown to stimulate ATPase activity and inhibit active calcium uptake in sarcoplasmic reticulum vesicles derived from rabbit fast skeletal muscle.
Abstract: Micromolar concentrations of mercury, silver, and other reagents known to react with sulfhydryl groups are shown to stimulate ATPase activity and inhibit active calcium uptake in sarcoplasmic reticulum vesicles derived from rabbit fast skeletal muscle. These effects are caused by a dramatic increase in the calcium permeability of the sarcoplasmic reticulum. Measurements of Ca2+ permeability were made using both isotopes and by spectrophotometric techniques using the Ca2+ indicator arsenazo III. Air oxidation of a sulfhydryl group to a disulfide group also leads to a large increase in the calcium permeability of the sarcoplasmic reticulum.
159 citations
TL;DR: Ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F) was newly synthesized as a fluorogenic reagent for thiol compounds and its reactivity to glutathione is superior to its 7-chloro analog, SBD-Cl.
TL;DR: In this article, the reaction between aldehydes and allylic or benzylic halides in the presence of samarium diiodide was studied and the reactivities of various nitrogen functional groups (imine, oxime, nitro, azo, cyano) towards SmI2 were also examined.
TL;DR: In this paper, the electrochemical detection of OPA-amino acids following reversed-phase HPLC is described, using fluorimetric and electrochemical analysis in series, and confirmation of the identities of the amino acids can be obtained in a single chromatographic run.
TL;DR: Barium manganate is a reagent capable of oxidizing organic substrates, converting alcohols to their corresponding carbonyl compounds, aromatic amines to their respective azo compounds, hydroquinone to p-benzoquinone, benzylamine to benzaldehyde, and triphenylphosphine to its oxide in good yields as discussed by the authors.
Abstract: Preparation of barium manganate in large-scale is discussed. It is also shown that this reagent is capable of oxidizing organic substrates, converting alcohols to their corresponding carbonyl compounds, aromatic amines to their corresponding azo compounds, hydroquinone to p-benzoquinone, benzylamine to benzaldehyde, and triphenylphosphine to its oxide in good yields. Saturated hydrocarbons, unsaturated hydrocarbons, unsaturated ketones, and saturated amines are not affected by this reagent in a reasonable reaction time.
TL;DR: Asymmetric reduction of prochiral aromatic ketones with the reagent prepared from (S)-(−)-2-amino-3-methyl-1,1-diphenylbutan-1-ol [(S]-(1)] and borane afforded the corresponding aromatic secondary alcohols in high optical (94−100% enantiomeric excess) and chemical (100%) yields as discussed by the authors.
Abstract: Asymmetric reduction of prochiral aromatic ketones with the reagent prepared from (S)-(–)-2-amino-3-methyl-1,1-diphenylbutan-1-ol [(S)-(1)] and borane afforded the corresponding aromatic secondary alcohols in high optical (94–100% enantiomeric excess) and chemical (100%) yields.
TL;DR: Cyanotrimethylsilane as mentioned in this paper adds to some ⇌,β-unsaturated ketones in conjugate manner under the catalytic action of Lewis acids such as triethylaluminium, aluminium chloride, and SnCl 2.
TL;DR: The mild conditions for phosphate determination employed in this method are unique, making it particularly suitable for the assay of orthophosphate in the presence of labile organophosphates.
TL;DR: Sodium perborate in acetic acid is an effective reagent for the oxidation of anilines to the corresponding nitroarenes; it is also highly effective for the oxidisation of sulphides to either sulphoxides or sulphones as discussed by the authors.
TL;DR: Aldehydes and ketones, when exposed to alkyl thiols or suitable alkane dithiols in presence of titanium tetrachloride, furnish near quantitative yields of the corresponding thioacetals.
TL;DR: The use of ruthenium tetroxide for the catalytic oxidation of coal molecules under mild conditions has been examined in this paper, where the selectivity of the reagent for aromatic nuclei has been assessed by a study of the oxidation of selected benzene, naphthalene and phenanthrene derivatives.
TL;DR: In this paper, a solventless vacuum reaction was used to fix monomeric and oligomeric siloxanes and also certain unsaturated hydrocarbons on either unsilanized or presilanized materials by cross-linking reactions with γ-radiation or peroxides.
TL;DR: In this article, the use of hydrazinedithiocarbonate (HDTC) for the removal of chloroacetyl and bromo-acetyl groups is described.
TL;DR: The reaction of 3,3,3-trifluoropropenes with disilane/tetrabutylammonium fluoride or silyllithium reagent gives γ,γ-difluoroallyl adducts of aldehydes and ketones with the aid of a catalyst such as potassium t-butoxide o r tris(diethylamino)sulfonium diffluorotrimethylsilicate as mentioned in this paper.
TL;DR: A simple, rapid, and sensitive spectrophotometric assay procedure for the determination of as low as 2 microM solutions of formaldehyde and acetaldehyde using an alkaline 4-amino-5-hydrazino-3-mercapto-1,2,4-triazole reagent is described.
TL;DR: In this article, the retention of 34 naphthalene and anthraquinone sulphonic acids was measured on C18 and C8 columns in mobile phases containing either an ion-pairing reagent or an inorganic salt in aqueous methanolic solvents.