Benjamin Basanta

TL;DR: This Perspective reviews tools developed over the past five years in the Rosetta software, including over 80 methods, and discusses improvements to the score function, user interfaces and usability.

TL;DR: The principles emerging from this analysis were used to design, de novo, a series of proteins with curved β sheets topped with α helices, showing that β-sheet curvature can be controlled with atomic-level accuracy.

TL;DR: This approach is expected to transform the design of small-molecule sensors and enzymes by enabling the creation of binding and active site geometries much more optimal for specific design challenges than is accessible by repurposing the limited number of naturally occurring NTF2-like proteins.

TL;DR: A hybrid approach involving NMR, hydrogen-deuterium exchange mass spectrometry, and modeling to provide the first residue-level characterization of the NTR is presented, supporting a model in which multiple grooves on the ACD interact with specific NTR regions, creating an ensemble of ‘quasi-ordered’ NTR states that can give rise to the known heterogeneity and plasticity of HSPB1.

TL;DR: An incoherent feed-forward loop (I-FFL) genetic circuit is designed to correlate its output pulse with the input signal in a relatively time-independent manner and generates a short-delayed reporter protein pulse that is dose dependent to the contaminant levels.