Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell

The emerging field of regenerative medicine will require a reliable source of stem cells in addition to biomaterial scaffolds and cytokine growth factors. Adipose tissue represents an abundant and accessible source of adult stem cells with the ability to differentiate along multiple lineage pathways. The isolation, characterization, and preclinical and clinical application of adipose-derived stem cells (ASCs) are reviewed in this article.

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Adipose-Derived Stem Cells for Regenerative Medicine

Hematopoietic stem cells (HSCs) supply all blood cells throughout life by making use of their self-renewal and multilineage differentiation capabilities. A monoclonal antibody raised to the mouse homolog of CD34 (mCD34) was used to purify mouse HSCs to near homogeneity. Unlike in humans, primitive adult mouse bone marrow HSCs were detected in the mCD34 low to negative fraction. Injection of a single mCD34(lo/-), c-Kit+, Sca-1(+), lineage markers negative (Lin-) cell resulted in long-term reconstitution of the lymphohematopoietic system in 21 percent of recipients. Thus, the purified HSC population should enable analysis of the self-renewal and multilineage differentiation of individual HSCs.

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Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell

Maintenance of the Hematopoietic Stem Cell Pool by CXCL12-CXCR4 Chemokine Signaling in Bone Marrow Stromal Cell Niches

MicroRNAs (miRNAs) are small noncoding RNAs that typically inhibit the translation and stability of messenger RNAs (mRNAs), controlling genes involved in cellular processes such as inflammation, cell-cycle regulation, stress response, differentiation, apoptosis, and migration. Thus, miRNAs have been implicated in the regulation of virtually all signaling circuits within a cell, and their dysregulation has been shown to play an essential role in the development and progression of cancer. Here, after a brief description of miRNA genomics, biogenesis, and function, we discuss the effects of miRNA dysregulation in the cellular pathways that lead to the progressive conversion of normal cells into cancer cells and the potential to develop new molecular miRNA-targeted therapies.

MicroRNAs in cancer.

Regulation of Hematopoietic Stem Cell Quiescence by the Novel Tumor Suppressor Dmtf1.

Genetic and Proteomic Analysis of Functionally Distinct Human Hematopoietic Stem Cells from Bone Marrow, Mobilized Peripheral Blood, and Cord Blood

Subcompartments of the G0 /G1 Phase of the Cell Cycle Functional Heterogeneity of Human CD34+ Cells Isolated in

Role of cd166 in multiple myeloma cell homing to the bone marrow microenvironment and disease progression

Re-entry of CD34+ cells into mitotic quiescence following in vitro proliferation is not sufficient for the restoration of primitive hematopoietic potential

Edward F. Srour

Papers

Regulation of Hematopoietic Stem Cell Quiescence by the Novel Tumor Suppressor Dmtf1.

Genetic and Proteomic Analysis of Functionally Distinct Human Hematopoietic Stem Cells from Bone Marrow, Mobilized Peripheral Blood, and Cord Blood

Subcompartments of the G0 /G1 Phase of the Cell Cycle Functional Heterogeneity of Human CD34+ Cells Isolated in

Role of cd166 in multiple myeloma cell homing to the bone marrow microenvironment and disease progression

Re-entry of CD34+ cells into mitotic quiescence following in vitro proliferation is not sufficient for the restoration of primitive hematopoietic potential