Edward F. Srour

TL;DR: In vivo administration of SCF led to an increase in both differentiated and primitive hematopoietic progenitor cells within the marrow, suggesting that in vivo SCF administration may be useful for improving the quality of bone marrow grafts to be used either for autologous or allogeneic bone marrow transplantation.

TL;DR: In this article, the authors demonstrate that CD166 is a functional hematopoietic stem cell (HSC) marker that identifies both murine and human long-term repopulating cells.

TL;DR: Investigation of the ability of transplanted BM cells to develop intracellular calcium transients in response to membrane depolarization in situ concludes that engrafted BM-derived cells lack attributes of functioning cardiomyocytes, calling into question the concept that adult BM cells can give rise to substantiveCardiomyocyte regeneration within the infarcted heart.

TL;DR: Homing of transplanted hematopoietic stem cells to recipient bone marrow is a critical step in engraftment and initiation of marrow reconstitution and the fate of marrow-homed cells shortly after transplantation and the rapidity at which they begin to proliferate in their new marrow microenvironment is confused.

TL;DR: This unit presents two protocols for differential staining, one using the metachromatic dye acridine orange (AO) and the other a combination of pyronin Y (PY) and Hoechst 33342, which has its advantages and limitations.