Gordon Ruthel

TL;DR: The authors demonstrate the benefits related to the statistical analyses of single-cell data to account for heterogeneity in the subcellular localization and whole-cell integrated intensity of the viral antigen staining pattern and show that image-based methods represent powerful screening tools for identifying antiviral compounds for highly pathogenic viruses.

TL;DR: This work reports that unlike authentic or inactivated Ebola and Marburg, filovirus‐derived virus‐like particles directly activated human natural killer cells in vitro, evidenced by pro‐inflammatory cytokine production and enhanced cytolysis of permissive target cells.

TL;DR: Data show that EBOV can use vps proteins independently of TSG101 for budding and reveal VPS4 as a potential target for filovirus therapeutics.

TL;DR: This review examines the relevant and available in vitro cell-based assays and in vivo assays for drug discovery and development, especially with regard to the potential for medium- to high-throughput automation and its use in identifying physiologically relevant inhibitors.

TL;DR: This is the first report demonstrating that reduced levels of host phosphatase CD45 modulate anthrax pathogenesis, and Mechanism-based studies suggest that the protection provided by reduced CD45 levels results from regulated immune cell homeostasis that may diminish the impact of apoptosis during the infection.