Gregory O. Staples

TL;DR: This work has developed a tool to computationally model LC/MS data to enable efficient profiling of glycans and aims to provide confident identification of true compounds in complex data sets that are not amenable to manual interpretation.

TL;DR: Chip based amide–silica hydrophilic interaction chromatography (HILIC) in a chip‐based format for LC/MS of heparin, heparan sulfate (HS) GAGs is an enabling technology for GAG glycomics profiling.

TL;DR: An interlaboratory study is performed to determine the ability of different laboratories to correctly characterize the differences between glycoforms from two different sources using mass spectrometry methods, and the relative strengths and weaknesses of top-down glycoproteomics, bottom-up glycoproteinomics, and glycomics methods.

TL;DR: A broad-based interlaboratory study of glycosylation profiles of a reference and modified IgG antibody involving 103 reports from 76 laboratories offers a view of the current state-of-the-art for biologic glyCosylation measurement and suggests a clear need for harmonization of gly cosylation analysis methods.

TL;DR: This study demonstrates the use of comprehensive 2D-LC separations involving cation-exchange (CEX) and reversed-phase (RP) separations in the first and second dimensions to compare 3 reference/biosimilar pairs of monoclonal antibodies that cover a range of similarity/disimilarity in a middle-up approach.