Mechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate
Gregory Huyer,Susana Liu,John F. Kelly,Jason Moffat,Paul Payette,Brian K. Kennedy,George Tsaprailis,Michael J. Gresser,Chidambaram Ramachandran +8 more
TLDR
The results show that vanadate is a competitive inhibitor for the protein-tyrosine phosphatase PTP1B, with a Ki of 0.38 ± 0.02 μM, and reducing agents such as dithiothreitol that are used in PTP assays to keep the catalytic cysteine reduced and active were found to convert pervanadate rapidly toVanadate.About:
This article is published in Journal of Biological Chemistry.The article was published on 1997-01-10 and is currently open access. It has received 801 citations till now. The article focuses on the topics: Vanadate.read more
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Increased insulin sensitivity and obesity resistance in mice lacking the protein tyrosine phosphatase-1B gene.
Mounib Elchebly,Paul Payette,Eva Michaliszyn,Wanda Cromlish,Susan Collins,Ailsa Lee Loy,Denis Normandin,Alan Cheng,Jean Himms-Hagen,Chi-Chung Chan,Chidambaram Ramachandran,Michael J. Gresser,Michel L. Tremblay,Brian P. Kennedy +13 more
TL;DR: In this article, the mouse homolog of the gene encoding PTP-1B yielded healthy mice that, in the fed state, had blood glucose concentrations that were slightly lower and concentrations of circulating insulin that were one-half those of their PTP−1B+/+ littermates.
Journal ArticleDOI
The chemistry and biochemistry of vanadium and the biological activities exerted by vanadium compounds.
TL;DR: Aqueous V(III) Chemistry 877 6.2.1.
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Reversible Inactivation of Protein-tyrosine Phosphatase 1B in A431 Cells Stimulated with Epidermal Growth Factor
TL;DR: The results indicate that the activation of a receptor tyrosine kinase by binding of the corresponding growth factor may not be sufficient to increase the steady state level of protein tyrosines phosphorylation in cells and that concurrent inhibition of protein-tyrosine phosphatases by H2O2 might also be required.
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Specific and reversible inactivation of protein tyrosine phosphatases by hydrogen peroxide: evidence for a sulfenic acid intermediate and implications for redox regulation.
John M. Denu,Kirk G. Tanner +1 more
TL;DR: This study explores the proposal that PTPs may be regulated by reversible reduction/oxidation involving cellular oxidants such as hydrogen peroxide (H2O2) and proposes a chemical mechanism for reversible inactivation involving a cysteine sulfenic acid intermediate.
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Hydrogen peroxide as second messenger in lymphocyte activation.
TL;DR: Data suggesting that by inhibiting protein tyrosine phosphatases, H2O2 plays an important role as a secondary messenger in the initiation and amplification of signaling at the antigen receptor is reviewed to explain why exposure of lymphocytes to H 2O2 can mimic the effect of antigen.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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Signal transduction by receptors with tyrosine kinase activity
Axel Ullrich,Joseph Schlessinger +1 more
TL;DR: Cet article synthese montre comment des recepteurs membranaires a activite tyrosine kinase peuvent etre impliques dans la transduction and notamment jouent le role de signal de the transduction.
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Requirement for Generation of H2O2 for Platelet-Derived Growth Factor Signal Transduction
TL;DR: The results suggest that H2O2 may act as a signal-transducing molecule, and they suggest a potential mechanism for the cardioprotective effects of antioxidants.
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Contributions of mass spectrometry to peptide and protein structure.
TL;DR: FAB mass spectrometry provides mainly molecular weight information which, in itself, often suffices to answer certain questions, particularly because the mass of many peptides can be determined directly from a single mass spectrum of a mixture.
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Effect of vanadate on elevated blood glucose and depressed cardiac performance of diabetic rats
TL;DR: Cardiac performance was depressed in the untreated diabetic animals, but the cardiac performance of the vanadate-treated diabetic animals was not significantly different from that of nondiabetic controls, so vanadates controlled the high blood glucose and prevented the decline in cardiac performance due to diabetes.