Jessica Antosiewicz-Bourget

TL;DR: Mapping genome-wide chromatin interactions in human embryonic stem cells and four human ES-cell-derived lineages reveals extensive chromatin reorganization during lineage specification, providing a global view of chromatin dynamics and a resource for studying long-range control of gene expression in distinct human cell lineages.

TL;DR: A cell culture system in which all protein reagents for liquid media, attachment surfaces and splitting are chemically defined is formed, and improved derivation efficiencies of vector-free human iPSCs with an episomal approach are demonstrated.

TL;DR: By comparing the chromatin-modification profiles and DNA methylomes in hESCs and primary fibroblasts, it is found that nearly one-third of the genome differs in chromatin structure.

TL;DR: It is found that promoters that are active in early developmental stages tend to be CG rich and mainly engage H3K27me3 upon silencing in nonexpressing lineages, while promoters for genes expressed preferentially at later stages are often CG poor and primarily employ DNA methylation upon repression.

TL;DR: By quantifying 3D cell migration, metabolic activity, vascular network disruption, and cytotoxicity, the PNVP model may be a useful tool to make physiologically relevant predictions of developmental toxicity.