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Monique M A Verstegen
Researcher at Erasmus University Rotterdam
Publications - 88
Citations - 4625
Monique M A Verstegen is an academic researcher from Erasmus University Rotterdam. The author has contributed to research in topics: Medicine & Organoid. The author has an hindex of 20, co-authored 58 publications receiving 3094 citations. Previous affiliations of Monique M A Verstegen include Hannover Medical School.
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Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver
Meritxell Huch,Helmuth Gehart,Ruben van Boxtel,Karien Hamer,Francis Blokzijl,Monique M A Verstegen,Ewa Ellis,Martien van Wenum,Sabine A. Fuchs,Joep de Ligt,Marc van de Wetering,Nobuo Sasaki,Susanne J. Boers,Hans Kemperman,Jeroen de Jonge,Jan N. M. IJzermans,Edward E. S. Nieuwenhuis,Ruurdtje Hoekstra,Stephen C. Strom,Robert R G Vries,Luc J. W. van der Laan,Edwin Cuppen,Hans Clevers +22 more
TL;DR: Conditions allowing long-term expansion of adult bile duct-derived bipotent progenitor cells from human liver opens up experimental avenues for disease modeling, toxicology studies, regenerative medicine, and gene therapy.
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Human primary liver cancer–derived organoid cultures for disease modeling and drug screening
Laura Broutier,Gianmarco Mastrogiovanni,Monique M A Verstegen,Hayley E. Francies,Lena Morrill Gavarró,Charles R. Bradshaw,George E. Allen,Robert Arnes-Benito,Olga Sidorova,Marcia P. Gaspersz,Nikitas Georgakopoulos,Bon-Kyoung Koo,Sabine Dietmann,Susan E. Davies,Raaj K. Praseedom,Ruby Lieshout,Jan N. M. IJzermans,Stephen J. Wigmore,Kourosh Saeb-Parsy,Mathew J. Garnett,Luc J. W. van der Laan,Meritxell Huch +21 more
TL;DR: The wide-ranging biomedical utilities of PLC-derived organoid models in furthering the understanding of liver cancer biology and in developing personalized-medicine approaches for the disease are demonstrated.
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Tissue-specific mutation accumulation in human adult stem cells during life
Francis Blokzijl,Joep de Ligt,Myrthe Jager,Valentina Sasselli,Sophie Roerink,Nobuo Sasaki,Meritxell Huch,Sander Boymans,Ewart W. Kuijk,Pjotr Prins,Isaac J. Nijman,Inigo Martincorena,Michal Mokry,Caroline L. Wiegerinck,Sabine Middendorp,Toshiro Sato,Gerald Schwank,Edward E. S. Nieuwenhuis,Monique M A Verstegen,Luc J. W. van der Laan,Jeroen de Jonge,Jan N. M. IJzermans,Robert G.J. Vries,Marc van de Wetering,Michael R. Stratton,Hans Clevers,Edwin Cuppen,Ruben van Boxtel +27 more
TL;DR: This work determines genome-wide mutation patterns in ASCs of the small intestine, colon and liver of human donors with ages ranging from 3 to 87 years by sequencing clonal organoid cultures derived from primary multipotent cells.
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Prime editing for functional repair in patient-derived disease models
Imre F. Schene,Indi P. Joore,Rurika Oka,Michal Mokry,Anke H.M. van Vugt,Ruben van Boxtel,Hubert P. J. van der Doef,Luc J. W. van der Laan,Monique M A Verstegen,Peter M. van Hasselt,Edward E. S. Nieuwenhuis,Edward E. S. Nieuwenhuis,Sabine A. Fuchs +12 more
TL;DR: Developing prime editing for primary adult stem cells grown in organoid culture models generates precise in-frame deletions in the gene encoding β‐catenin that result in proliferation independent of Wnt-stimuli, mimicking a mechanism of the development of liver cancer.
Journal ArticleDOI
Overcoming promoter competition in packaging cells improves production of self-inactivating retroviral vectors.
Axel Schambach,D Mueller,Melanie Galla,Monique M A Verstegen,Gerard Wagemaker,Rainer Loew,Christopher Baum,Christopher Baum,Jens Bohne +8 more
TL;DR: This study introduces a new generation of efficient gammaretroviral SIN vectors as a platform for further optimizations of retroviral vector performance and reveals that promoter competition is a rate-limiting step of SIN vector production.