V
Valentina Sasselli
Researcher at Utrecht University
Publications - 10
Citations - 2870
Valentina Sasselli is an academic researcher from Utrecht University. The author has contributed to research in topics: Enteric nervous system & Stem cell. The author has an hindex of 9, co-authored 10 publications receiving 2402 citations. Previous affiliations of Valentina Sasselli include Royal Netherlands Academy of Arts and Sciences & National Institute for Medical Research.
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Journal ArticleDOI
Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients
Gerald Schwank,Bon-Kyoung Koo,Valentina Sasselli,Johanna F. Dekkers,Inha Heo,Turan Demircan,Nobuo Sasaki,Sander Boymans,Edwin Cuppen,Cornelis K. van der Ent,Edward E. S. Nieuwenhuis,Jeffrey M. Beekman,Hans Clevers +12 more
TL;DR: The CRISPR/Cas9 genome editing system is used to correct the CFTR locus by homologous recombination in cultured intestinal stem cells of CF patients and the corrected allele is expressed and fully functional as measured in clonally expanded organoids.
Journal ArticleDOI
Tissue-specific mutation accumulation in human adult stem cells during life
Francis Blokzijl,Joep de Ligt,Myrthe Jager,Valentina Sasselli,Sophie Roerink,Nobuo Sasaki,Meritxell Huch,Sander Boymans,Ewart W. Kuijk,Pjotr Prins,Isaac J. Nijman,Inigo Martincorena,Michal Mokry,Caroline L. Wiegerinck,Sabine Middendorp,Toshiro Sato,Gerald Schwank,Edward E. S. Nieuwenhuis,Monique M A Verstegen,Luc J. W. van der Laan,Jeroen de Jonge,Jan N. M. IJzermans,Robert G.J. Vries,Marc van de Wetering,Michael R. Stratton,Hans Clevers,Edwin Cuppen,Ruben van Boxtel +27 more
TL;DR: This work determines genome-wide mutation patterns in ASCs of the small intestine, colon and liver of human donors with ages ranging from 3 to 87 years by sequencing clonal organoid cultures derived from primary multipotent cells.
Journal ArticleDOI
Use of CRISPR-modified human stem cell organoids to study the origin of mutational signatures in cancer.
Jarno Drost,Ruben van Boxtel,Francis Blokzijl,Tomohiro Mizutani,Nobuo Sasaki,Valentina Sasselli,Joep de Ligt,Sam Behjati,Sam Behjati,Judith E. Grolleman,Tom van Wezel,Serena Nik-Zainal,Serena Nik-Zainal,Roland P. Kuiper,Edwin Cuppen,Hans Clevers +15 more
TL;DR: A strategy that can be used to explore the origin of cancer-associated mutational signatures is developed and it is found that mutation accumulation in organoids deficient in the mismatch repair gene MLH1 is driven by replication errors and accurately models the mutation profiles observed in mismatch repair–deficient colorectal cancers.
Journal ArticleDOI
The enteric nervous system.
TL;DR: The molecular mechanisms underlying key developmental processes that affect enteric NCC proliferation and survival are reviewed and the need to translate the wealth of data gleaned from animal studies to the clinical area is discussed to better understand, and develop treatments for, congenital human diseases affecting the ENS.
Journal ArticleDOI
Ascl2 Acts as an R-spondin/Wnt-Responsive Switch to Control Stemness in Intestinal Crypts
Jurian Schuijers,Jan Philipp Junker,Michal Mokry,Pantelis Hatzis,Bon-Kyoung Koo,Bon-Kyoung Koo,Valentina Sasselli,Laurens G. van der Flier,Edwin Cuppen,Alexander van Oudenaarden,Hans Clevers +10 more
TL;DR: This work shows that Ascl2 is regulated in a direct autoactivatory loop, leading to a distinct on/off expression pattern, and that Wnt/R-spondin can activate this regulatory loop.