Peter G. Schultz

TL;DR: A new, highly efficient suppressor plasmid, pUltra, harboring a single copy each of the tRNA and aaRS expression cassettes that exhibits higher suppression activity than its predecessors is described, able to efficiently incorporate up to three UAAs within the same protein at levels up to 30% of the level of wild-type protein expression.

TL;DR: The fluorescent amino acid l-(7-hydroxycoumarin-4-yl) ethylglycine 1 has been genetically encoded in E. coli in response to the amber TAG codon and should provide a useful probe of protein localization and trafficking, protein conformation changes, and protein-protein interactions.

TL;DR: Using a novel genetic selection, a series of synthetase mutants are identified that selectively charge the amber suppresor tRNA with the C8 amino acid, alpha-aminocaprylic acid, and the photocaged amino acids, o-nitrobenzyl cysteine, allowing them to be inserted into proteins in yeast in response to the amber nonsense codon, TAG.

TL;DR: The development of a high-throughput circadian functional assay system that consists of luminescent reporter cells, screening automation, and a data analysis pipeline is reported, finding that small molecule inhibitors of glycogen synthase kinase 3 (GSK-3) consistently caused a strong short period phenotype in contrast to the well-known period lengthening by lithium.

TL;DR: The results suggest that isoniazid is oxidized by the catalase-peroxidase to a reactive species that may act by inactivating an essential enzyme of M. tuberculosis.