Satya Khuon

TL;DR: In this article, a 3D view of early secretory compartments in mammalian cells with isotropic resolution and precise protein localization using whole-cell, focused ion beam scanning electron microscopy with cryo-structured illumination microscopy and live-cell synchronized cargo release approaches is provided.

TL;DR: Using spectroscopic photon localization microscopy, simultaneous multi-colour super-resolution imaging of microtubules and mitochondria in COS-7 cells is demonstrated and it is shown that background autofluorescence can be identified through its distinct emission spectra.

TL;DR: Evidence is shown that the binding kinetics of the antigen engaging the T cell receptor influences the nanoscale actin organization and mechanics of the immune synapse, suggesting that tuning actin dynamics in response to antigen kinetics may be a mechanism that allows T cells to adjust the lengthscale and timescale of T cell receptors signaling.

TL;DR: A new open source cluster analysis method for 3D SMLM data, free of user definable parameters, relying on a model-based Bayesian approach which takes full account of the individual localisation precisions in all three dimensions is presented.

TL;DR: An enhanced force measurement technique combining 3D super-resolution fluorescence structural illumination microscopy and traction force microscopy (3D-SIM-TFM) offering increased spatiotemporal resolution is introduced, opening-up unprecedented insights into physiological three-dimensional force production in living cells.