Soah Lee

TL;DR: It is found that protein accumulation increased substantially above a critical mesh size, which was distinct from the protein diffusivity trend, highlighting the importance of using protein accumulation as a parameter to better predict the cell fates in addition to protein diffusion, a parameter commonly reported by researchers studying protein diffusion in hydrogels.

TL;DR: A molecular interplay is uncovered that enables massive hiPSC-CM expansion for large-scale drug screening and tissue engineering applications and shows uncompromised cellular functionality after expansion.

TL;DR: A new scalable method using Faraday waves to enable rapid aggregation of human induced pluripotent stem cell-derived cardiomyocytes into predefined 3D constructs that resembles the cellular architecture of a native heart tissue for diverse basic research and clinical applications is reported.

TL;DR: iPSC-CMs carrying MYBPC3 PTC mutations displayed aberrant calcium signaling and molecular dysregulations in the absence of significant haploinsufficiency of MYB PC3 protein, providing the first evidence of the direct connection between the chronically activated nonsense-mediated decay pathway and HCM disease development.

TL;DR: A critical role for endocardium is revealed in HLHS etiology and a rationale for considering endocardial function in regenerative strategies is provided, consistent with recently described de novo HLHS mutations associated with abnormal end Cardiomyocyte proliferation and maturation.