V
Verena Hirschfeld
Researcher at University of Lübeck
Publications - 8
Citations - 968
Verena Hirschfeld is an academic researcher from University of Lübeck. The author has contributed to research in topics: Single-molecule FRET & Förster resonance energy transfer. The author has an hindex of 5, co-authored 7 publications receiving 787 citations. Previous affiliations of Verena Hirschfeld include University of Zurich.
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From the Cover: Charge interactions can dominate the dimensions of intrinsically disordered proteins
Sonja Müller-Späth,Andrea Soranno,Verena Hirschfeld,Hagen Hofmann,Stefan Rüegger,Luc Reymond,Daniel Nettels,Benjamin Schuler +7 more
TL;DR: Forster resonance energy transfer was used in this article to investigate the influence of charged residues on the dimensions of unfolded and intrinsically disordered proteins, and it was shown that charge-balanced polypeptides can exhibit an additional collapse at low ionic strength, as predicted by polyampholyte theory.
Journal ArticleDOI
Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
Björn Hellenkamp,Björn Hellenkamp,Sonja Schmid,Sonja Schmid,Olga Doroshenko,Oleg Opanasyuk,Ralf Kühnemuth,Soheila Rezaei Adariani,Benjamin Ambrose,Mikayel Aznauryan,Anders Barth,Victoria Birkedal,Mark E. Bowen,Hongtao Chen,Thorben Cordes,Thorben Cordes,Tobias Eilert,Carel Fijen,Christian Gebhardt,Markus Götz,Giorgos Gouridis,Giorgos Gouridis,Enrico Gratton,Taekjip Ha,Pengyu Hao,Christian A. Hanke,Andreas Hartmann,Jelle Hendrix,Jelle Hendrix,Lasse L. Hildebrandt,Verena Hirschfeld,Johannes Hohlbein,Boyang Hua,Christian G. Hübner,Eleni Kallis,Achillefs N. Kapanidis,Jae-Yeol Kim,Georg Krainer,Georg Krainer,Don C. Lamb,Nam Ki Lee,Edward A. Lemke,Edward A. Lemke,Brié Levesque,Marcia Levitus,James J. McCann,Nikolaus Naredi-Rainer,Daniel Nettels,Thuy T.M. Ngo,Ruoyi Qiu,Nicole C. Robb,Carlheinz Röcker,Hugo Sanabria,Michael Schlierf,Tim Schröder,Benjamin Schuler,Henning Seidel,Lisa Streit,Johann Thurn,Philip Tinnefeld,Philip Tinnefeld,Swati Tyagi,Niels Vandenberk,Andrés Manuel Vera,Keith Weninger,Bettina Wünsch,Inna S. Yanez-Orozco,Jens Michaelis,Claus A. M. Seidel,Timothy D. Craggs,Timothy D. Craggs,Thorsten Hugel +71 more
TL;DR: A multi-laboratory study finds that single-molecule FRET is a reproducible and reliable approach for determining accurate distances in dye-labeled DNA duplexes.
Journal ArticleDOI
Charge interactions can dominate the dimensions of intrinsically disordered proteins
Sonja Müller-Späth,Andrea Soranno,Verena Hirschfeld,Verena Hirschfeld,Hagen Hofmann,Stefan Rüegger,Stefan Rüegger,Luc Reymond,Luc Reymond,Daniel Nettels,Benjamin Schuler +10 more
TL;DR: This work uses single-molecule Forster resonance energy transfer to investigate the influence of charged residues on the dimensions of unfolded and intrinsically disordered proteins and finds that IDPs can exhibit a prominent expansion at low ionic strength that correlates with their net charge.
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Conformational plasticity and dynamics in the generic protein folding catalyst SlyD unraveled by single-molecule FRET.
Dana Kahra,Michael Kovermann,Christian Löw,Verena Hirschfeld,Caroline Haupt,Jochen Balbach,Christian G. Hübner +6 more
TL;DR: A model where the conformational dynamics is used to search the conformation enabling the chemical step is proposed, which also explains the remarkable substrate promiscuity connected with a high efficiency of this class of peptidyl-prolyl cis-trans isomerases.
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Excited-state annihilation reduces power dependence of single-molecule FRET experiments
Daniel Nettels,Dominik Haenni,Sacha Maillot,Moussa Gueye,Anders Barth,Verena Hirschfeld,Christian G. Hübner,Jérémie Léonard,Benjamin Schuler +8 more
TL;DR: It is shown that excited-state annihilation processes are an essential cause of singlet-singlet annihilation, and both SSA and STA have a pronounced effect on the overall FRET process and reduce the power dependence of the observed FRET efficiencies.