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Showing papers by "Colorado State University published in 1983"



Journal ArticleDOI
TL;DR: In this paper, the correlation of 1H and 15N chemical shifts based on zero and double quantum NMR is discussed. And the indirect determination of nitrogen shifts from the proton signals provides a dramatic gain in sensitivity over direct observation of 15N signals, and examples are given of applications of the methods to samples with natural abundance 15N concentration and to a sample of 0.7 mM 65% 15N enriched tRNA in H2O.

1,236 citations



Journal ArticleDOI
Ad Bax1
TL;DR: In this article, the authors proposed an approach to homonuclear broadband decoupling for semiselective heteronuclear J spectroscopy, where all protons which are not directly attached to a 13C nucleus experience an effect as if a nonselective 180" pulse were applied, while protons bonded to a r3C nucleus are not affected.

445 citations


Journal ArticleDOI
TL;DR: Mycorrhizal plants derived significantly (P= 0.01) more 16N, from both N sources, than did control plants and no correlations were found between the 16N content of mycorrhIZal plants and any parameter in the organic-N treatment.
Abstract: SUMMARY Celery (Apium graveolens L.) plants were grown in pots in which the root system was separated from the soil in a side chamber by a fine mesh screen. The side chamber was treated with either an organic (ground plant tissue) or inorganic [(NH4)2SO4] source of 15N. Mycorrhizal (Glomus mosseae) and control (non-mycorrhizal) plants were exposed to 15N over a period of 30 days (inorganic-15N) or 88 days (organic-16N). Mycorrhizal and control plants did not differ in shoot dry weight or shoot P content. Dry weight of root was reduced in the mycorrhiza treatments. Mycorrhizal plants derived significantly (P= 0.01) more 16N, from both N sources, than did control plants. In the inorganic-N treatment, 15N in mycorrhizal plants was significantly (P= 0.001) and positively correlated with percent mycorrhizal fungal colonization (r= 0.58), number of hyphal crossings (±10 μ diameter) through the mesh into the area of 15N placement (r= 0.76), total length of hyphae per gram of soil (r= 0.74), and length of hyphae of 5 μ diameter in the soil (r= 0.77). No correlations were found between the 16N content of mycorrhizal plants and any parameter in the organic-N treatment. The 16N content of control plants was not correlated with hyphal length in the outer chamber and there were no hyphal crossings of the size ( 10 μ diameter) which was counted for the mycorrhiza treatments. The presence of the organic matter (ground plant tissue) increased the total length of saprophytic hyphae per gram of soil but decreased the number of vesicular-arbuscular mycorrhizal fungal hyphae crossing into the area of 16N placement. The mean flux of N through the hyphae of G. mosseae was 7.42 × 10−8 mol N cm−2 s−1 for the inorganic-N treatment over a 30-day period, and 1.74 × 10−8 mol N cm−2 s−1 for the organic-N treatment over an 88-day period.

401 citations


Book
01 Jan 1983

387 citations


Journal ArticleDOI
TL;DR: In this paper, the seasonal dynamics of soil nematodes and root biomass were examined from under western wheatgrass (Agropyron smithii) and little bluestem (Andropogon scoparius) from a heavily grazed prairie dog colony occupied for 5 to 10 years and an adjacent lightly grazed, uncolonized area in Wind Cave National Park, South Dakota, USA.
Abstract: Seasonal dynamics of soil nematodes and root biomass were examined from under western wheatgrass (Agropyron smithii) and little bluestem (Andropogon scoparius) from a heavily grazed prairie dog (Cynomys ludovicianus) colony occupied for 5 to 10 years and an adjacent lightly grazed, uncolonized area in Wind Cave National Park, South Dakota, USA. Nematodes were differentiated into classes of plant-parasitic Tylenchida and Dorylaimida and nonparasitic Dorylamida and Rhabditida. Root-feeding nematodes were generally more numerous from A. smithii than from A. scoparius, while nonparasitic populations were not different in soil from beneath the two plant species. Rhabditida, parasitic Dorylaimida and Tylenchida (from A. scoparius only) were more numerous on the prairie dog colony than from the uncolonized site, but nonparasitic Dorylaimida populations did not differ between the two areas. Mean total (live plus dead) root biomass beneath A. scoparius and A. smithii on the prairie dog colony averaged 71% and 81%, respectively, of values from the uncolonized area. Estimated consumption by root-feeding nematodes averaged 12.6% and 5.8% of annual net root production in the upper 10 cm from the prairie dog colony and uncolonized site, respectively. We conclude that, because of microhabitat modification or reductions in plant resistance to nematodes, heavy grazing by aboveground herbivores apparently facilitates grazing by belowground herbivores. Because heavily grazed plants have less roots than lightly grazed or ungrazed plants, the impact of root-feeding nematodes on primary producers is likely to be greatest in heavily grazed grasslands.

350 citations


Journal ArticleDOI
TL;DR: In this paper, the dynamics of tropical cyclone motion were investigated by solving for instantaneous motion tendencies using the divergent barotropic vorticity equation on a beta plane, and two methods of solution were presented a direct analytic solution for a constant basic current, and a simple numerical solution for more general conditions.
Abstract: The dynamics of tropical cyclone motion are investigated by solving for instantaneous motion tendencies using the divergent barotropic vorticity equation on a beta plane. Two methods of solution are presented a direct analytic solution for a constant basic current, and a simple numerical solution for more general conditions. These solutions indicate that cyclone motion can be accurately prescribed by a nonlinear combination of two processes. 1) an interaction between the cyclone and its basic current (the well known steering concept), and 2) an interaction with the Earth's vorticity field which causes a westward deviation from the pure steering flow. The nonlinear manner in which these two processes combine with the effect of asymmetries in the steering current raise some interesting questions on the way in which cyclones of different characteristics interact with their environment, and has implications for tropical cyclone forecasting and the manner in which forecasting techniques are derived.

333 citations


Journal ArticleDOI
TL;DR: In this paper, the authors examined components of teacher judgements that an intervention is either acceptable or unacceptable, including risk to the target child, amount of teacher time required, effects of the intervention on other children, and teacher skill required.
Abstract: This study examined components of teacher judgements that an intervention is either acceptable or unacceptable. A total of 180 preservice and student teachers were asked to evaluate the acceptability of six different interventions. The evaluations of acceptability were assessed using a 20–item rating scale. A factor analysis of the rating scale yielded one major dimension, a general acceptability factor, and four secondary dimensions of intervention acceptability: risk to the target child, amount of teacher time required, effects of the intervention on other children, and amount of teacher skill required. Results are discussed in terms of designing interventions that are both effective and usable by classroom teachers.

326 citations


Journal ArticleDOI
TL;DR: In this paper, a marine stratocumulus model has been developed which has four major sub-models: a one-dimensional version of the CSU cumulus model, a partially-diagnostic higher-order turbulence model, an atmospheric radiation model for both short-wave and long-wave radiation, and a partial condensation scheme and cloud fractional parameterization.
Abstract: A marine stratocumulus model has been developed which has four major sub-models: (1) a one-dimensional version of the CSU cumulus model, (2) a partially-diagnostic higher-order turbulence model, (3) an atmospheric radiation model for both short-wave and long-wave radiation, and (4) a partial condensation scheme and cloud fractional parameterization. A set of numerical experiments have been performed to study the interactions among the turbulence, the long-wave radiation, the short-wave radiation, and the sub-grid condensation processes. The results indicate that surface sensible eddy heat flux and not radiative cooling is the major control on the rate of cloud-top entrainment. Cloud-top radiation cooling occurs principally within the upper part of the mixed layer. However, for the stratocumulus with numerous towers penetrated into the capping inversion, most of the long-wave radiation occurs within the capping inversion. It is found that cloud-top radiation cooling is balanced by turbulence transport of sensible heat from cloud-base levels.

282 citations



Journal ArticleDOI
01 Oct 1983-Nature
TL;DR: A marine algal sapropel from Mangrove Lake, Bermuda, by 13C NMR and stable carbon isotopic methods showed that precursors of aquatic kerogen (insoluble, macromolecular, paraffinic humic substances) are primary components of algae and possibly associated bacteria.
Abstract: Studies of a marine algal sapropel from Mangrove Lake, Bermuda, by 13C NMR and stable carbon isotopic methods show that precursors of aquatic kerogen (insoluble, macromolecular, paraffinic humic substances) are primary components of algae and possibly associated bacteria and that these substances survive microbial decomposition and are selectively preserved during early diagenesis.

Journal ArticleDOI
07 Oct 1983-Science
TL;DR: Repeat geodetic surveys show uplift of the Monroe and Wiggins anticlines in Louisiana and Mississippi and there are deformed Quaternary terraces, which indicate long-term deformation in the valleys of the alluvial rivers that cross these structures, and floodplain and channel convexities that provide evidence of modern deformation.
Abstract: Repeat geodetic surveys show uplift of the Monroe and Wiggins anticlines in Louisiana and Mississippi. There are deformed Quaternary terraces, which indicate long-term deformation in the valleys of the alluvial rivers that cross these structures, and there are floodplain and channel convexities that provide evidence of modern deformation. In addition, the channels show significant variations of morphology (sinuosity, gradient, and depth) and behavior appropriate to reaches of increased and decreased valley slope. These alluvial rivers are adjusting to modern deformation and their adjustment confirms two geodetic leveling anomalies.

Journal ArticleDOI
TL;DR: The trisaccharide determinant of phenolic glycolipid I is specific in its structure, serological activity, and, to a lesser extent, the antibody class it evokes.
Abstract: The serological activities of the specific phenolic glycolipid I from Mycobacterium leprae, its dissected parts, and related glycolipids from other mycobacteria were examined by enzyme-linked immunosorbent assay against hyperimmune anti-M. leprae rabbit antiserum and sera from patients with leprosy and other mycobacterial diseases. High anti-phenolic glycolipid I immunoglobulin M antibodies were found in 23 of 24 (96%) of lepromatous leprosy patients on short term chemotherapy and in 8 of 13 tuberculoid leprosy patients (62%). Sera from patients with tuberculosis or atypical mycobacterial infections were devoid of anti-phenolic glycolipid I activity. The structurally related phenolic glycolipids from Mycobacterium kansasii and Mycobacterium bovis and the aglycone segments of the M. leprae product showed no significant activity. Thus, the trisaccharide determinant of phenolic glycolipid I is specific in its structure, serological activity, and, to a lesser extent, the antibody class it evokes.

Journal ArticleDOI
01 Feb 1983-Ecology
TL;DR: In this article, a synthesis based on data generated by the International Biological Pro- gram (IBPG) deals with the relationships among biotic and abiotic factors at the ecosystem level.
Abstract: This paper, a synthesis based on data generated by the International Biological Pro- gram, deals with the relationships among biotic and abiotic factors at the ecosystem level. Emphasis is placed on aboveground net primary production (ANPP), a major component of energy that drives ecosystem processes, and on potential evapotranspiration (PET), the abiotic variable most often used to explain variation in ANPP. The question addressed is: can ANPP be related to combinations of biotic and abiotic factors such that the relationships are independent of ecosystem type, whether it be forest, grassland, or desert'? ANPP as a function of peak foliar standing crop (FSC) was best explained by models which showed a reduction in ANPP/FSC as FSC increased. Thus, deserts had a higher ANPP per unit of FSC than did other systems. As expected, photosynthetic efficiency (PE) was highest for forests, -ZOO times greater than for deserts. However, when PE was evaluated per unit of foliage, the differences in PE of ecosystems were much less. In fact, a hot-desert site had the highest PE/FSC. In terms of a theoretical maximum, the PE of forests was only 6-25% of the maximum value. Systems with nearly steady-state aboveground standing crop (ASC) showed an exponential decrease with decreased water availability (potential evapotranspiration minus precipitation). For these same systems, the ratio of ANPP to ASC increased with decreased water availability, suggesting that water-stressed systems need more energy from ANPP to drive internal processes. A model predicting ANPP of desert-shortgrass steppes was structured in terms of FSC, water availability, and temperature. The predictive power was found to be very highs and the model was successfully validated in two of three cases with an independent data set. A model predicting ANPP of forests was structured in terms of FSC, radiation, ASC, and temperature. The deviation of the observed ANPP relative to that calculated was 17%. Deviations from predicted values were highest for deciduous stands with high ANPP and low FSC. Most relationships exhibited good correlations between ANPP and the various independent vari- ables including both biotic, abiotic, and combinations of the two. However, in many instances the data tended to be grouped by ecosystem type, suggesting that variation in ANPP can be reduced if ecosystem type is an added independent variable. It was surprising to find that, with the limits of our data, differences in ANPP at the ecosystem level are not glaring, especially considering that soil factors were not included in our analyses. When considering the broad range of genotypes in each ecosystem, and the much broader genotypic range representing all ecosystems, the control that native ecosystems have over abiotic factors in producing ANPP is evident but not large.

Journal ArticleDOI
09 Dec 1983-Science
TL;DR: The initial rate of break rejoining was similar to the rate of increase in survival after incubation because of the repair of potentially lethal damage and is also in close agreement with recently reported values for the rejoining of double-strand breakage in DNA.
Abstract: A method was developed for the high-resolution measurement of breaks in prematurely condensed chromosomes at the G1 phase of the cell cycle. The dose response for fragments (breaks) produced immediately after x-irradiation of confluent cultures of normal human cells was linear down to 10.9 rad (0.109 Gy) and extrapolated to zero effect at zero dose. The curve had a slope of 0.063 breaks per cell per rad, which is at least an order of magnitude greater than that for breaks scored in the same cells after they have progressed to mitosis following subculture. When incubated at 37 degrees C half of the breaks disappeared in 2 hours. A slower, perhaps nonrejoining component was apparent at later incubation times. The initial rate of break rejoining was similar to the rate of increase in survival after incubation because of the repair of potentially lethal damage and is also in close agreement with recently reported values for the rejoining of double-strand breakage in DNA.

Journal ArticleDOI
TL;DR: In this article, the authors consider the problem of the linear response of a stratified, equatorial, β-plane model atmosphere to specified transient sources of heat and momentum, and use a finite Sturm-Liouville transform in z, a Fourier transform in x, and a generalized Hermite transform in y.
Abstract: We consider the problem of the linear response of a stratified, equatorial, β-plane model atmosphere to specified transient sources of heat and momentum. The method of solution involves transforms in all three spatial coordinates. A finite Sturm-Liouville transform is used in z, a Fourier transform in x, and a generalized Hermite transform in y. The resulting spectral equations can then be solved analytically for a specified forcing. Of particular interest is the case of a Gaussian-shaped heat source centered at latitude yo and with e-folding radius a. The heat source is transient and has time scale 1/α. Using the Parceval relation we compute how the forced energy is partitioned between Kelvin, mixed Rossby-gravity, Rossby and gravity modes as a function of a, yo, α. Model results using a heat source centered at 11°S with an e-folding radius of 750 km and a time scale of about a day indicate that many aspects of the summertime upper tropospheric circulation over South America can be explained by ...

Journal ArticleDOI
TL;DR: Prairie dogs facilitate bison habitat selection for a shortgrass successional stage in this mixed-grass community by causing a broad array of compositional, structural, and nutritional changes in the vegetation.
Abstract: Studies were conducted during the 1979 growing season to examine how North American bison (Bison bison) use prairie dog (Cynomys ludovicianus) colonies in Wind Cave National Park, South Dakota. Objectives included (1) determining whether bison selected for prairie dog towns parkwide; (2) characterizing in greater detail bison use patterns of a 36-ha colony in Pringle Valley as a function of time since prairie dog colonization; and (3) relating these bison use patterns to measured changes in structure and nutritional value of vegetation on and off the dog town.During midsummer, prairie dog towns were one of the most frequently used habitats by bison parkwide. Day-long observations at Pringle Valley revealed that bison exerted strong selection (nearly 90% of all habitat use and feeding time) for the dog town, which occupied only 39% of the valley. While there, they partitioned their use of the colony by grazing in moderately affected areas (occupied 26 years occupation).Prairie dogs facilitate bison habitat selection for a shortgrass successional stage in this mixed-grass community by causing a broad array of compositional, structural, and nutritional changes in the vegetation.

Journal ArticleDOI
TL;DR: The feasibility and advantages of using atomic vapor cells as blocking filters for measuring atmospheric parameters are demonstrated with a numerical example worked out in detail.
Abstract: A new lidar technique for measuring the profiles of backscatter ratio, atmospheric visibility, and atmospheric temperature is proposed. Based on the theory of high resolution Rayleigh/Mie scattering, the feasibility and advantages of using atomic vapor cells as blocking filters for measuring atmospheric parameters are demonstrated with a numerical example worked out in detail. Ten percent accuracy in determining backscatter ratio and visibility can be achieved easily. With a SNR of 300, temperature of 1 K accuracy can be measured directly along with the backscatter ratio to a better accuracy of ±1%. Using a large lidar system and assuming 50-km visibility, the proposed technique can be applied to measure backscatter ratio and temperature profiles simultaneously for a 10-km path with 30-m depth resolution in 3 min. With higher SNR the atmospheric pressure profile can also be determined.

Journal ArticleDOI
01 Apr 1983-Planta
TL;DR: As a result of initial experiments to optimize both media for E callus production and media for plant regeneration, callus derived in six passages from an average of 26 seeds could produce about 1,000 regenerated plants.
Abstract: By visual examination of calli derived from germinating seeds of wheat, oats, rice, proso millet, and pearl millet it has been possible to visually select embryogenic (E) callus which, on transfer to a regeneration medium, forms plants an average of 33 times more frequently than non-embryogenic (NE) callus of equal mass. Embryogenic callus consists of small isodiametric cells averaging 31 μm in diameter; NE callus consists of long tubular cells averaging 52 μm in width and 355 μm in length. Production of E callus is in many cases promoted by media containing 2,4-di- or 2,4,5-trichlorophenoxyacetic acid (2,4-D or 2,4,5-T) plus indole-3-acetic acid or tryptophan+kinetin. Production on NE callus is promoted by media containing 2,4-D or 2,4,5-T alone. As a result of initial experiments to optimize both media for E callus production and media for plant regeneration, callus derived in six passages from an average of 26 seeds could produce about 1,000 regenerated plants.

Journal ArticleDOI
TL;DR: In this paper, the authors derived a method for finding small amplitude high frequency solutions to hyperbolic systems of quasilinear partial differential equations, where each high frequency wave displays nonlinear distortion of the wave profile and shocks may form.
Abstract: : In this paper we derive a method for finding small amplitude high frequency solutions to hyperbolic systems of quasilinear partial differential equations. Our solution is the sum of two parts: (i) a superposition of small amplitude high frequency waves; (ii) a slowly varying 'mean solution'. Each high frequency wave displays nonlinear distortion of the wave profile and shocks may form. Shock conditions are derived for conservative systems. Different high frequency waves do not interact provided the frequencies and wave numbers of two waves are not linearly related to those of a third. The mean solution is found by solving a linear partial differential equation. This method generalizes Whitham's nonlinearization technique 9 for single waves, to problems where many waves are present. We obtain these results by generalizing a scheme first proposed by Choquet-Bruhat 1 which employs the method of multiple scales. (Author)

Book ChapterDOI
TL;DR: Although the stimulation of photosynthesis and translocation of current photosynthate to the root system by mycorrhiza formation was consistent with the source-sink concept of sink demand, foliar N and P concentrations were also greater in myCorrhizal plants.
Abstract: Studies examined net photosynthesis (Pn) and dry matter production of mycorrhizal and nonmycorrhizal Pinus taeda at 6 intervals over a 10-month period. Pn rates of mycorrhizal plants were consistently greater than nonmycorrhizal plants, and at 10 months were 2.1-fold greater. Partitioning of current photosynthate was examined by pulse-labelling with 14CO2 at each of the six time intervals. Mycorrhizal plants assimilated more 14CO2, allocated a greater percentage of assimilated 14C to the root systems, and lost a greater percentage of 14C by root respiration than did nonmycorrhizal plants. At 10 months, the quantity of 14CO2 respired by roots per unit root weight was 3.6-fold greater by mycorrhizal than nonmycorrhizal plants. Although the stimulation of photosynthesis and translocation of current photosynthate to the root system by mycorrhiza formation was consistent with the source-sink concept of sink demand, foliar N and P concentrations were also greater in mycorrhizal plants.

Journal ArticleDOI
TL;DR: In this paper, a new two-dimensional approach for obtaining the anisotropy information is proposed, where the spinning axis of the sample is flipped from 90 to 54.7° between the evolution and detection periods.

Journal ArticleDOI
TL;DR: The 13C CP-MAS experiment as discussed by the authors is the most widely applied solid state NMR experiment in recent years, but it suffers from a limitation that it eliminates the potentially useful information embodied in the chemical shift anisotropy pattern.

Journal ArticleDOI
TL;DR: Photosynthesis and regrowth were compared over a 10-day period following defoliation of about 75% of the tillers of western wheatgrass plants collected from a black-tailed prairie dog town and a grazing exclosure at Wind Cave National Park, South Dakota, and rates did not differ between plants of the two populations.
Abstract: Photosynthesis and regrowth were compared over a 10-day period following defoliation of about 75% of the tillers of western wheatgrass (Agropyron smithii) plants collected from a black-tailed prairie dog (Cynomys ludovicianus) town and a grazing exclosure at Wind Cave National Park, South Dakota. Prior to defoliation, dog town plants had more tillers, but fewer leaves per tiller, shorter and narrower leaf blades, more horizontal leaves, and higher leaf blade/leaf sheath ratios than plants from the grazing exclosure. Rates of net photosynthesis (PN) did not differ significantly among plants of the two populations, either prior to or following defoliation. From Days 2–10 following defoliation, PN of remaining undamaged leaves averaged 104% of predefoliation rates while PN of similar leaves on non-defoliated plants declined steadily with time. averaging only 79% predefoliation rates during this period. Following defoliation, transpiration rates followed similar trends to CO2 exchange, and rates did not differ between plants of the two populations. Absolute rates of leaf elongation and shoot production were greater in plants from the exclosure. However, defoliation of plants from the exclosure population resulted in a 20% reduction in their cumulative shoot dry weight, while cumulative shoot dry weight of plants from the prairie dog town was not significantly affected by defoliation. This apparent ability of plants from the prairie dog town population to withstand defoliation better than plants from the exclosure was atributed to factors such as the higher leaf blade/leaf sheath ratio and more horizontal leaf angles of plants from the former population.

Journal ArticleDOI
TL;DR: The complexity of a periodic sequence is defined to be the least number of stages in a linear feedback shift register that generates periodic sequences.
Abstract: The complexity of a periodic sequence (s) is defined to be the least number of stages in a linear feedback shift register that generates (s)

Journal ArticleDOI
TL;DR: A statistically significant correlation to body size was found for the aortic, left atrial, left ventricular, septal, and posterior wall dimensions and the mitral valve amplitude of motion in young healthy dogs studied by M-mode echocardiography.
Abstract: Twenty young healthy dogs weighing from 9.8 to 28.6 kg were studied by M-mode echocardiography. Parameters were measured and statistically evaluated to determine whether a correlation to body surface area existed. A statistically significant correlation to body size was found for the aortic, left atrial, left ventricular, septal, and posterior wall dimensions and the mitral valve amplitude of motion. In addition, normal values not correlated to body surface area are presented with their means and standard deviations. These values include velocity of circumferential fiber shortening, ejection time, percent systolic thickening of septum and posterior wall, percent change in minor diameter, selected dimension ratios, and mitral valve velocities

Journal ArticleDOI
TL;DR: In this article, two-dimensional solitary and periodic waves in water of finite depth were considered and it was shown that elevation solitary waves cannot be obtained as the continuous limit of periodic waves as the wavelength tends to infinity.
Abstract: Two-dimensional solitary and periodic waves in water of finite depth are considered. The waves propagate under the combined influence of gravity and surface tension. The flow, the surface profile and the phase velocity are functions of the amplitude of the wave and the parameters l = λ/H and τ = T/ρgH2. Here λ is the wavelength, H the depth, T the surface tension, ρ the density and g the acceleration due to gravity. For . In addition, it is shown that elevation solitary waves cannot be obtained as the continuous limit of periodic waves as the wavelength tends to infinity. Graphs of the results are included.

Journal ArticleDOI
TL;DR: A new function was empirically derived to describe nymphal development of the migratory grasshopper Melanoplus sanguinipes and was shown to be preferable to the other models in the high- and low-temperature range.
Abstract: A new function was empirically derived to describe nymphal development of the migratory grasshopper Melanoplus sanguinipes (F.). This nonlinear equation was to fit to a large data set from the literature and compared with two previously proposed nonlinear models and the commonly used degree-day linear model. The functions were also compared in their ability to predict development under conditions of alternating temperatures, including temperatures that would often occur in the field. Although all models fit the data well at intermediate temperatures, the new model was shown to be preferable to the other models in the high- and low-temperature range.

Journal ArticleDOI
TL;DR: The prepubertal period, which starts at 10 to 12 wk in well-fed Holstein bulls, is characterized by profound changes of hypothalamic, pituitary, and gonadal function that culminate in puberty.