Showing papers by "National Institute of Oceanography, India published in 2006"

Molecular Biomarkers: Their significance and application in marine pollution monitoring

Abstract: This paper presents an overview of the significance of the use of molecular biomarkers as diagnostic and prognostic tools for marine pollution monitoring. In order to assess the impact of highly persistent pollutants such as polychlorinated biphenyls (PCB), polychlorinated dibenzo–dioxins (PCDD), polychlorinated dibenzo–furans (PCDF), polynuclear aromatic hydrocarbons (PAH), tributyltin (TBT) and other toxic metals on the marine ecosystem a suite of biomarkers are being extensively used worldwide. Among the various types of biomarkers, the following have received special attention: cytochrome P4501A induction, DNA integrity, acetylcholinesterase activity and metallothionein induction. These biomarkers are being used to evaluate exposure of various species of sentinel marine organisms (e.g. mussels, clams, oysters, snails, fishes, etc.) to and the effect of various contaminants (organic xenobiotics and metals) using different molecular approaches [biochemical assays, enzyme linked immuno-sorbent assays (ELISA), spectrophotometric, fluorometric measurement, differential pulsed polarography, liquid chromatography, atomic absorption spectrometry]. The induction of the biotransformation enzyme, cytochrome P4501A in fishes (Callionymus lyra, Limanda limanda, Serranus sp., Mullus barbatus) and mussels (Dreissena polymorpha) by various xenobiotic contaminants such as PCBs, PAHs, PCDs is used as a biomarker of exposure to such organic pollutants. The induction of cytochrome P4501A is involved in chemical carcinogenesis through catalysis of the covalent bonding of organic contaminants to a DNA strand leading to formation of DNA adduct. Measurement of the induction of cytochrome P4501A in terms of EROD (7-ethoxy resorufin O-deethylase) activity is successfully used as a potential biomarker of exposure to xenobiotic contaminants in marine pollution monitoring.

Effect of dietary probiotic Biogen® supplementation as a growth promoter on growth performance and feed utilization of Nile tilapia Oreochromis niloticus (L.)

Abstract: Probiotic microbial feed supplements are gaining wide acceptance in livestock production, and may be applicable to aquaculture production systems. The present study was conducted to examine probiotic treatment in the fingerling diet of Nile tilapia Oreochromis niloticus (L.). A total of 240 of Nile tilapia fingerlings (weight ranged from 22.96 to 26.40 g) were divided into five experimental groups. The experiment was conducted for 120 days. Experimental diets were identical in all, except for the variation in probiotic levels. A probiotic (Biogen®) was used at 0% (diet 1), 0.5% (diet 2), 1.5% (diet 3), 2.0% (diet 4) and 2.5% (diet 5) inclusion rates in the experimental diets. The growth performance and nutrient utilization of Nile tilapia including weight gain, specific growth rate, protein efficiency ratio, protein productive value and energy retention were significantly (P≤0.01) higher in the treatment receiving probiotic (Biogen®) than the control diet. No differences were observed for moisture, ash and protein content (P≤0.01) among the experimental diets. The lowest gross energy and lipid contents were recorded for fish fed the diet containing 0.5% Biogen® (P≤0.01). The production performance and subsequent cost–benefit analyses clearly indicated that the diets containing probiotic biogen recorded the highest net return and the lowest total cost compared with the control diet.

Climate Fluctuations of Tropical Coupled Systems—The Role of Ocean Dynamics

Abstract: The tropical oceans have long been recognized as the most important region for large-scale ocean–atmosphere interactions, giving rise to coupled climate variations on several time scales. During the Tropical Ocean Global Atmosphere (TOGA) decade, the focus of much tropical ocean research was on understanding El Nino–related processes and on development of tropical ocean models capable of simulating and predicting El Nino. These studies led to an appreciation of the vital role the ocean plays in providing the memory for predicting El Nino and thus making seasonal climate prediction feasible. With the end of TOGA and the beginning of Climate Variability and Prediction (CLIVAR), the scope of climate variability and predictability studies has expanded from the tropical Pacific and ENSO-centric basis to the global domain. In this paper the progress that has been made in tropical ocean climate studies during the early years of CLIVAR is discussed. The discussion is divided geographically into three tropical ocean basins with an emphasis on the dynamical processes that are most relevant to the coupling between the atmosphere and oceans. For the tropical Pacific, the continuing effort to improve understanding of large- and small-scale dynamics for the purpose of extending the skill of ENSO prediction is assessed. This paper then goes beyond the time and space scales of El Nino and discusses recent research activities on the fundamental issue of the processes maintaining the tropical thermocline. This includes the study of subtropical cells (STCs) and ventilated thermocline processes, which are potentially important to the understanding of the low-frequency modulation of El Nino. For the tropical Atlantic, the dominant oceanic processes that interact with regional atmospheric feedbacks are examined as well as the remote influence from both the Pacific El Nino and extratropical climate fluctuations giving rise to multiple patterns of variability distinguished by season and location. The potential impact of Atlantic thermohaline circulation on tropical Atlantic variability (TAV) is also discussed. For the tropical Indian Ocean, local and remote mechanisms governing low-frequency sea surface temperature variations are examined. After reviewing the recent rapid progress in the understanding of coupled dynamics in the region, this study focuses on the active role of ocean dynamics in a seasonally locked east–west internal mode of variability, known as the Indian Ocean dipole (IOD). Influences of the IOD on climatic conditions in Asia, Australia, East Africa, and Europe are discussed. While the attempt throughout is to give a comprehensive overview of what is known about the role of the tropical oceans in climate, the fact of the matter is that much remains to be understood and explained. The complex nature of the tropical coupled phenomena and the interaction among them argue strongly for coordinated and sustained observations, as well as additional careful modeling investigations in order to further advance the current understanding of the role of tropical oceans in climate.

Surface freshwater from Bay of Bengal runoff and Indonesian Throughflow in the tropical Indian Ocean

Abstract: According to recent estimates, the annual total continental runoff into the Bay of Bengal (BoB) is about 2950 km 3, which is more than half that into the entire tropical Indian Ocean (IO). Here we use climatological observations to trace the seasonal pathways of near surface freshwater from BoB runoff and Indonesian Throughflow (ITF) by removing the net contribution from precipitation minus evaporation. North of 20 degrees S, the amount of freshwater from BoB runoff and ITF changes with season in a manner consistent with surface currents from drifters. BoB runoff reaches remote regions of the Arabian Sea; it also crosses the equator in the east to join the ITF. This freshwater subsequently flows west across the southern tropical IO in the South Equatorial Current.

Distribution and seasonal variation of trace metals in surface sediments of the Mandovi estuary, west coast of India

Abstract: The concentration and distribution of selected trace metals in surface sediments of the Mandovi estuary were studied to determine the extent of anthropogenic inputs from mining activities and to estimate the effects of the monsoon on geochemical processes in this tropical estuarine system. Analysis of bulk sediments from the Mandovi estuary shows that the concentrations of iron, manganese, cobalt, copper, zinc and lead vary from 2.2 to 49.7%;

α-L-arabinofuranosidases : the potential applications in biotechnology

Abstract: Recently, α-l-arabinofuranosidases (EC3.2.1.55) have received increased attention primarily due to their role in the degradation of lignocelluloses as well as their positive effect on the activity of other enzymes acting on lignocelluloses. As a result, these enzymes are used in many biotechnological applications including wine industry, clarification of fruit juices, digestion enhancement of animal feedstuffs and as a natural improver for bread. Moreover, these enzymes could be used to improve existing technologies and to develop new technologies. The production, mechanisms of action, classification, synergistic role, biochemical properties, substrate specificities, molecular biology and biotechnological applications of these enzymes have been reviewed in this article.

Coastal versus open-ocean denitrification in the Arabian Sea

Abstract: . The Arabian Sea contains one of the three major open-ocean denitrification zones in the world. In addition, pelagic denitrification also occurs over the inner and mid-shelf off the west coast of India. The major differences between the two environments are highlighted using the available data. The perennial open-ocean system occupies two orders of magnitude larger volume than the seasonal coastal system, however, the latter offers more extreme conditions (greater nitrate consumption leading to complete anoxia). Unlike the open-ocean system, the coastal system seems to have undergone a change (i.e., it has intensified) over the past few decades presumably due to enhanced nutrient loading from land. The two systems also differ from each other with regard to the modes of nitrous oxide (N2O) production: In the open-ocean suboxic zone, an accumulation of secondary nitrite (NO2−) is invariably accompanied by depletion of N2O whereas in the coastal suboxic zone high NO2− and very high N2O concentrations frequently co-occur, indicating, respectively, net consumption and net production of N2O by denitrifiers. The extents of heavier isotope enrichment in the combined nitrate and nitrite (NO3−+NO2−) pool and in N2O in reducing waters appear to be considerably smaller in the coastal region, reflecting more varied sources/sinks and/or different isotopic fractionation factors.

Fungi in deep-sea sediments of the Central Indian Basin

Abstract: Although a great amount of information is available on bacteria inhabiting deep-sea sediments, the occurrence of fungi in this environment has been poorly studied and documented. We report here the occurrence of fungi in deep-sea sediments from ∼5000 m depth in the Central Indian Basin (9–16°S and 73–76°E). A total of 181 cultures of fungi, most of which belong to terrestrial sporulating species, were isolated by a variety of isolation techniques. Species of Aspergillus and non-sporulating fungi were the most common. Several yeasts were also isolated. Maximum species diversity was observed in 0–2 cm sections of the sediment cores. Direct staining of the sediments with Calcofluor, a fluorescent optical brightener, revealed the presence of fungal hyphae in the sediments. Immunofluorescence using polyclonal antibodies raised against a deep-sea isolate of Aspergillus terreus (# A 4634) confirmed its presence in the form of hyphae in the sub-section from which it was isolated. A total of 25 representative species of fungi produced substantial biomass at 200 bar pressure at 30° as well as at 5 °C. Many fungi showed abnormal morphology at 200 bar/5 °C. A comparison of terrestrial isolates with several deep-sea isolates indicated that the former could grow at 200 bar pressure when growth was initiated with mycelial inocula. However, spores of a deep-sea isolate A. terreus (# A 4634), but not the terrestrial ones, showed germination at 200 bar pressure and 30 °C. Our results suggest that terrestrial species of fungi transported to the deep sea are initially stressed but may gradually adapt themselves for growth under these conditions.

Recent morphodynamics of the Indus delta shore and shelf

Abstract: In natural conditions, the Indus River had one of the largest sediment loads in the world, building an extensive delta on the high-energy coast of the Arabian Sea. However, water and sediment discharge have been drastically altered in the Indus since the early 1960s, when several barrages were built along the river to feed the world's largest irrigation system. A digital terrain model based on detailed 19th century surveys has been constructed to assess the morphology of the Indus shelf. Comparison of the digital terrain model to a 1950s Pakistani bathymetric survey allowed an estimation of the natural sedimentation regime before extensive human-induced changes. Digital analysis of the Indus delta coastline based on satellite imagery was used to explore the effects of the drastic decrease in sediment delivery following extensive dam building. The Indus Canyon is a dominant feature of the region dissecting the shelf to within 20 m water depth and 3.5 km of the coast. Theoretical considerations based on estimates of the relative importance of wave energy vs. fluvial sediment delivery suggest that the Indus delta should develop a mid-shelf subaqueous clinoform. Instead, the Indus shelf exhibits a compound clinoform morphology. A shallow delta front clinoform extends along the entire delta coast from the shoreline to the 10–25 m water depth. A mid-shelf clinoform developed probably as a prodelta clinoform between ∼30 and 90 m water depth. The advanced position of the mid-shelf clinoform east of the Indus Canyon might reflect either a prolonged sediment delivery from the Indus River in that area compared to the shelf west of the canyon or the presence of a relict pre-Holocene mid-shelf delta. A distinct lobe of the mid-shelf clinoform developed along the Kutch (Kachchh) coast probably as sediment advected alongshore was redeposited on the mid-shelf by strong offshore-directed tidal currents at the Gulf of Kutch mouth. Accumulation and erosion between 1895/96 and 1952/54 occurred primarily on the delta front clinoform, but also on the prodelta clinoform sector covered by both the surveys. During that time period, at the active Indus mouths, the delta front clinoform has built directly into the Indus Canyon, where sedimentation rates exceeded 50 cm/year. A sediment budget for the shelf for the 1895/96–1952/54 period suggests that the previous estimate of an Indus sediment discharge rate of 250 million tons per year in natural conditions is probably a minimum estimate. For the same time interval, the shoreline advanced along most of the delta coast. The progradation rate at the active mouths along the central delta coast surpassed 100 m/year. Following the 80% reduction in sediment discharge after the late 1950s, the deltaic shoreline along the central delta coast started to recede at average rates of ∼50 m/year. The abandoned delta shore (southeastern and northwestern sectors of the delta coast) remained largely progradational over the same period, with the southeastern sector prograding at an even greater rate than before. This differential behavior of the delta shoreline suggests a significant role for delta front sediment transfer processes in the evolution of abandoned deltaic coast.

Steps in the construction of underwater coral nursery, an essential component in reef restoration acts

Abstract: Many coral reefs worldwide are rapidly declin- ing, but efficient restoration techniques are not yet available. Here, we evaluate methodologies for reef res- toration based on the ''gardening concept''. A floating mid-water prototype nursery was placed at 6 m depth (14 m above sea-bottom) within the nutrient-enriched environment of a fish farm (Eilat, Red Sea). Ten colonies from five branching coral species provided 6,813 frag- ments (0.5-3 cm height). The fragments, each attached to a plastic pin, were inserted into plastic nets that were tied to a rope-net floating nursery. After 144 nursery days, only 13.1% of the fragments died and 21.2% were de- tached by mechanical forces. Small colonies ready for transplantation developed within 144-200 days. Ramets' ecological volumes increased 13-46 folds and their heights by a factor of 3.5. After 306 days, the ecological volumes of the colonies increased 147-163 fold as com- pared to original volumes (revealing a daily growth rate constant of 1.67% during the first 5-10 months) and height values by a factor of six. Building and maintenance costs of the nursery were low. This nursery prototype demonstrates the feasibility of the coral ''gardening concept'' by fulfilling several important needs, namely, mass production of coral colonies at low costs, high survivorship, fast growth, short nursery phase and im- proved methodologies for handling farmed colonies.

Metal and antibiotic-resistance in psychrotrophic bacteria from Antarctic Marine waters

Abstract: In the wake of the findings that Antarctic krills concentrate heavy metals at ppm level, (Yamamoto et al. 1987), the Antarctic waters from the Indian side were examined for the incidence of metal and antibiotic-resistant bacteria during the austral summer (13th Indian Antarctic expedition) along the cruise track extending from 50 degrees S and 18 degrees E to 65 degrees S and 30 degrees E. The bacterial isolates from these waters showed varying degrees of resistance to antibiotics (Chloramphenicol, ampicillin, streptomycin, tetracycline and kanamycin) and metals (K(2)CrO(4), CdCl(2), ZnCl(2) and HgCl(2)) tested. Of the isolates screened, about 29% and 16% were resistant to 100 ppm of cadmium and chromium salt respectively. Tolerance to lower concentration (10 ppm) of mercury (Hg) was observed in 68% of the isolates. Depending on the antibiotics the isolates showed different percentage of resistance. Multiple drug and metal-resistance were observed. High incidence of resistance to both antibiotics and metals were common among the pigmented bacterial isolates. Increased resistance decreased the ability of bacteria to express enzymes. The results reiterate previous findings by other researchers that the waters of southern ocean may not be exempt from the spread of metal and antibiotic-resistance.

Distribution and statistical analysis of leachable and total heavy metals in the sediments of the Suez Gulf.

Abstract: The concentrations of nine heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pd and Zn) in the labile and total fractions of muddy and sandy sediment samples collected from twelve sites in Suez Gulf during April 1999 were studied to evaluate the pollution status of the Suez Gulf. The enrichment factors (EF) for each element were calculated. There are extremely high concentrations of Cd, Ni, Pb and slightly concentration of Cr and Cu in both muddy and sandy sediments. The concentration of Zn was moderately high and can be considered as seriously contaminate Metal pollution index (MPI) shows high values ranged between 46 to 156 and 40 to 232 for both sandy and muddy sediments, respectively. Concentrations of heavy metals were normalized against iron for total fraction in both of sandy and muddy sediments. Principal component analysis (PCA) was studied on the data matrix obtained and represented three-factor model explaining 92.22% for labile and 88.82% for total fractions of muddy sediment. The main source of contamination is the offshore oil fields and industrial wastes. This is largely a result of ineffective and inefficient operation equipment, illegal discharge of dirty ballast water from tankers and lack of supervision and prosecution of offenders.

Impact of freshwater influx on microzooplankton mediated food web in a tropical estuary (Cochin backwaters – India)

Abstract: The diversity, abundance and biomass of microzooplankton in Cochin backwaters were studied for the first time during pre-summer monsoon to peak of summer monsoon (April–July 2003) to understand the impact of large freshwater influx. Microzooplankton abundance and biomass were highest during pre-summer monsoon (av. 3817 ind. L−1 and 146 μg C L−1) that declined with the onset (av. 2052 ind. L−1 and 45 μg C L−1) and peak (av. 409 ind. L−1 and 10 μg C L−1) summer monsoon. Species diversity, richness and evenness of microzooplankton also showed similar trends as that of abundance and biomass. Grazing experiment showed that microzooplankton consumes 43 ± 1% of the daily phytoplankton standing stock during the high saline condition (27.5). Low abundance of microzooplankton during summer monsoon period (1/8 of the pre-summer monsoon value) along with the concomitant occurrence of low mesozooplankton (1/8 times of pre-summer monsoon value) suggests that there could be a general lack of planktonic grazers. This would result in a weak transfer of primary and bacterial carbon to higher trophic levels, eventually leaving behind much unconsumed basic food in the estuary during summer monsoon. Thus a major portion of the primary carbon either settles down or gets transported to the coastal regions during monsoon. High flushing of Cochin backwaters also facilitates faster removal of primary producers to the coastal regions during monsoon.

Removal of Methylene Blue from aqueous solution by marine green alga Ulva lactuca

Abstract: Biosorption of colours is an important technology for treatment of different types of industrial wastewaters containing dyes. The objective of this study was to convert green alga Ulva lactuca to dye adsorbents for wastewater treatment. The importance of commonly available green alga Ulva lactuca was investigated as viable biomaterials for the biological treatment of synthetic basic blue 9 (5-ch1oro-N,N,N ′,N ′-tetramethyl-5λ4-phenothiazine-3,7-diamine) effluents. The results obtained from the batch experiments revealed the ability of the green algae to remove the basic blue 9, and this was dependent on the dye concentration, pH, and algal biomass. We investigated the equilibrium and kinetics of adsorption, and the Langmuir and Freundlich equations were used to fit the equilibrium isotherm. The adsorption isotherm of basic blue 9 followed both the Langmuir and Freundlich models with a correlation coefficient of ∼0.96–0.99, and the adsorption kinetics followed the pseudo-second-order model (R 2=1.0). The m...

Sea level changes along the Indian coast: Observations and projections

Abstract: Besides causing a sea level rise, there is a co ncern that global warming may cause changes in the occurrence of extreme events such as cyclones. Extreme events in the coastal regions occur in the form of storm surges, apart from tsunamis. The occurrence of extreme events is more or less a regional phenomenon, caused by changes in regional climate. The occurrence of storm surges is co mmon to the countries surrounding the Bay of Bengal, as the Bay is the hotbed of generation of tropical cyclones. Any i ncrease in the frequency or intensity of tropical distu rbances in future will cause increased damages to life and property in the coastal regions. The present paper mainly deals with the occurrence of tropical cyclones in the Bay of Bengal and associated storm surges in a future climate scenario. Projections for the future are needed for decision making by planners and policy makers. Future projections are made for different scenarios 2

Seasonal variability of the mixed layer in the central Bay of Bengal and associated changes in nutrients and chlorophyll

Abstract: Hydrographic data from National Oceanographic Data Center (NODC) and Responsible National Oceanographic Data Centre (RNODC) were used to study the seasonal variability of the mixed layer in the central Bay of Bengal (8–20°N and 87–91°E), while meteorological data from Comprehensive Ocean Atmosphere Data Set (COADS) were used to explore atmospheric forcing responsible for the variability. The observed changes in the mixed-layer depth (MLD) clearly demarcated a distinct north–south regime with 15°N as the limiting latitude. North of this latitude MLD remained shallow (∼20 m) for most of the year without showing any appreciable seasonality. Lack of seasonality suggests that the low-salinity water, which is perennially present in the northern Bay, controls the stability and MLD. The observed winter freshening is driven by the winter rainfall and associated river discharge, which is advected offshore under the prevailing circulation. The resulting stratification was so strong that even a 4 °C cooling in sea-surface temperature (SST) during winter was unable to initiate convective mixing. In contrast, the southern region showed a strong semi-annual variability with deep MLD during summer and winter and a shallow MLD during spring and fall intermonsoons. The shallow MLD in spring and fall results from primary and secondary heating associated with increased incoming solar radiation and lighter winds during this period. The deep mixed layer during summer results from two processes: the increased wind forcing and the intrusion of high-salinity waters of Arabian Sea origin. The high winds associated with summer monsoon initiate greater wind-driven mixing, while the intrusion of high-salinity waters erodes the halocline and weakens the upper-layer stratification of the water column and aids in vertical mixing. The deep MLD in the south during winter was driven by wind-mixing, when the upper water column was comparatively less stable. The deep MLD between 15 and 17°N during March–May cannot be explained in the context of local atmospheric forcing. We show that this is associated with the propagation of Rossby waves from the eastern Bay. We also show that the nitrate and chlorophyll distribution in the upper ocean during spring intermonsoon is strongly coupled to the MLD, whereas during summer river runoff and cold-core eddies appear to play a major role in regulating the nutrients and chlorophyll.

Glycolipids from the red alga Chondria armata (Kütz.) Okamura

Abstract: Three distinct fractions containing polar glycolipids (PF 1-3 ) were isolated from the chloroform soluble fraction of crude methanolic extract of red alga Chondria armata (Kutz.) Okamura on gel chromatography over Sephadex LH 20 . Their structure was elucidated by multidimensional nuclear magnetic resonance (NMR) techniques like 1 H, 1 H correlation spectroscopy (COSY), 1 H, 1 H total COSY (TOCSY), 1 H, 13 C heteronuclear multiple quantum coherence (HMQC), and 1 H, 13 C heteronuclear multiple bond correlation (HMBC) complemented by electrospray ionization mass spectrometry (ESI-MS) in the positive ion mode. The coupling constant of the anomeric proton in 1 H NMR spectrum and sign of rotation indicated an exclusive configuration of the sugar molecules in the glycerolipids. Major glycolipids were identified as (2R)-2-O-(5,8,11,14-eicosatetranoyl)-3-O-α-D-galactopyranosyl-sn-glycerol (GL 2 ), its pentacetate (GL 1 ), and (2R)-1-O-(palmitoyl)-2-O-(5,8,11, 14,17-eicosapentanoyl)-3-O-β-D-galactopyranosyl-sn-glycerol (GL 3 ). Each was methanolysed to give the same galactosylglycerol which on ESI-MS provided a pseudomolecular ion at m/z 309 representing deacylated glycolipid with the sodiated sugar moiety. Additionally, six minor glycolipids were also identified on the basis of ESI-MS. These include a 1,2-di-O-acyl-3-O-(acyl-6'-galactosyl)-glycerol (GL 1a ), sulfonoglycolipids 2-O-palmitoyl-3-O-(6'-sulfoquinovopyranosyl)-glycerol (GL 2a ) and its ethyl ether derivative (GL 2b ), 1-oleoyl-2-palmitoyl-3-O-galactosyl glycerol (GL 3a ), and 1,2-diacyl phosphatidyl glycerol (GL 3b ). GL 1 , GL 1a , and GL 2b are new to the literature. The novelty of the remaining identified compounds lies in the diversity of their fatty acid composition. Antimicrobial properties of these glycolipids against pathogens were evaluated. The yeast Candida albicans and the bacteria Klebsiella sp. were as sensitive as the standard Nystatin and antibiotic Streptomycin against PF 3 . Considerable activity was expressed by the same metabolite against the fungus Cryptococcus neoformans as compared to the control. Weak activity against the bacteria Shigella flexineri and Vibrio cholerae and the fungus Aspergillus fumigatus was also observed. Fraction PF 2 was weakly active against some strains whereas all of them were resistant to its acetyl derivative PF 1 . Antimicrobial activity of glycolipids is being reported here for the first time.