SRM University

About: SRM University is a education organization based out in Chennai, India. It is known for research contribution in the topics: Computer science & Population. The organization has 10787 authors who have published 11704 publications receiving 103767 citations. The organization is also known as: Sri Ramaswamy Memorial University.

Assessment of groundwater potential zones using GIS technique

Abstract: A case study was conducted to find out the groundwater potential zones in Kattakulathur block, Tamil Nadu, India with an aerial extent of 360.60 km2. The thematic maps such as geology, geomorphology, soil hydrological group, land use / land cover and drainage map were prepared for the study area. The Digital Elevation Model (DEM) has been generated from the 10 m interval contour lines (which is derived from SOI, Toposheet 1:25000 scale) and obtained the slope (%) of the study area. The groundwater potential zones were obtained by overlaying all the thematic maps in terms of weighted overlay methods using the spatial analysis tool in ArcGIS 9.2. During weighted overlay analysis, the ranking has been given for each individual parameter of each thematic map and weights were assigned according to the influence such as soil −25%, geomorphology − 25%, land use/land cover −25%, slope − 15%, lineament − 5% and drainage / streams − 5% and find out the potential zones in terms of good, moderate and poor zones with the area of 49.70 km2, 261.61 km2 and 46.04 km2 respectively. The potential zone wise study area was overlaid with village boundary map and the village wise groundwater potential zones with three categories such as good, moderate and poor zones were obtained. This GIS based output result was validated by conducting field survey by randomly selecting wells in different villages using GPS instruments. The coordinates of each well location were obtained by GPS and plotted in the GIS platform and it was clearly shown that the well coordinates were exactly seated with the classified zones.

Therapeutic potential of pterostilbene against pancreatic beta‐cell apoptosis mediated through Nrf2

Abstract: Background and Purpose Nuclear factor erythroid 2-related factor 2 (Nrf2) is considered to be a ‘master regulator’ of the antioxidant response as it regulates the expression of several genes including phase II metabolic and antioxidant enzymes and thus plays an important role in preventing oxidative stress-mediated disorders, including diabetes. In this study, for the first time, we investigated the protective properties of a naturally available antioxidant, pterostilbene (PTS), against pancreatic beta-cell apoptosis and the involvement of Nrf2 in its mechanism of action. Experimental Approach Immunoblotting and quantitative reverse transcriptase (qRT)-PCR analysis were performed to identify PTS-mediated nuclear translocation of Nrf2 protein and the following activation of target gene expression, respectively, in INS-1E cells. In addition, an annexin-V binding assay was carried out to identify the apoptotic status of PTS-treated INS-1E cells, while confirming the anti-apoptotic potential of Nrf2 by qRT-PCR analysis of the expressions of both pro- and anti-apoptotic genes. Key Results PTS induced significant activation of Nrf2, in dose- and time-dependent manner, in streptozotocin-treated INS-1E rat pancreatic beta-cells. Furthermore, PTS increased the expression of target genes downstream of Nrf2, such as heme oxygenase 1 (HO1), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), that confer cellular protection. PTS also up-regulated the expression of anti-apoptotic gene, Bcl-2, with a concomitant reduction in pro-apoptotic Bax and caspase-3 expression. Conclusion and Implications Collectively, our findings indicate the therapeutic potential of Nrf2 activation by PTS as a promising approach to safeguard pancreatic beta-cells against oxidative damage in diabetes.