Showing papers by "Université de Sherbrooke published in 1998"

Pathogenic implications of mutations in the tau gene in pallido-ponto-nigral degeneration and related neurodegenerative disorders linked to chromosome 17

Abstract: Pallido-ponto-nigral degeneration (PPND) is one of the most well characterized familial neurodegenerative disorders linked to chromosome 17q21-22. These hereditary disorders are known collectively as frontotemporal dementia (FTD) and parkinsonism linked to chromosome 17 (FTDP-17). Although the clinical features and associated regional variations in the neuronal loss observed in different FTDP-17 kindreds are diverse, the diagnostic lesions of FTDP-17 brains are tau-rich filaments in the cytoplasm of specific subpopulations of neurons and glial cells. The microtubule associated protein (tau) gene is located on chromosome 17q21-22. For these reasons, we investigated the possibility that PPND and other FTDP-17 syndromes might be caused by mutations in the tau gene. Two missense mutations in exon 10 of the tau gene that segregate with disease, Asn279(Lys) in the PPND kindred and Pro301(Leu) in four other FTDP-17 kindreds, were found. A third mutation was found in the intron adjacent to the 3' splice site of exon 10 in patients from another FTDP-17 family. Transcripts that contain exon 10 encode tau isoforms with four microtubule (MT)-binding repeats (4Rtau) as opposed to tau isoforms with three MT-binding repeats (3Rtau). The insoluble tau aggregates isolated from brains of patients with each mutation were analyzed by immunoblotting using tau-specific antibodies. For each of three mutations, abnormal tau with an apparent Mr of 64 and 69 was observed. The dephosphorylated material comigrated with tau isoforms containing exon 10 having four MT-binding repeats but not with 3Rtau. Thus, the brains of patients with both the missense mutations and the splice junction mutation contain aggregates of insoluble 4Rtau in filamentous inclusions, which may lead to neurodegeneration.

Yeast Ku as a Regulator of Chromosomal DNA End Structure

Abstract: During telomere replication in yeast, chromosome ends acquire an S-phase-specific overhang of the guanosine-rich strand. Here it is shown that in cells lacking Ku, a heterodimeric protein involved in nonhomologous DNA end joining, these overhangs are present throughout the cell cycle. In vivo cross-linking experiments demonstrated that Ku is bound to telomeric DNA. These results show that Ku plays a direct role in establishing a normal DNA end structure on yeast chromosomes, conceivably by functioning as a terminus-binding factor. Because Ku-mediated DNA end joining involving telomeres would result in chromosome instability, our data also suggest that Ku has a distinct function when bound to telomeres.

Viscosity-enhancing admixtures for cement-based materials — An overview

Abstract: Viscosity-enhancing admixtures, also known as anti-washout admixtures, are water-soluble polymers that increase the viscosity and cohesion of cement-based materials. Such enhancement of the liquid-phase viscosity is essential in flowable systems in order to reduce the rate of separation of material constituents and improve the homogeneity and performance of the hardened product. Viscosity-enhancing admixtures are mostly used along with a high-range water reducer to obtain a highly fluid, yet cohesive cement-based material that can flow readily into place with minimal separation of the various constituents of different densities and minimal intermixing with the surrounding water whenever cast under water. This paper reviews the types and modes of action of commonly used viscosity-enhancing admixtures and highlights their influence on the rheological properties of water and cement paste. An overview of the influence of various types of viscosity-enhancing admixture on high-range water reducer demand, resistance to water dilution, static and forced bleeding, segregation, settlement, setting time, and air entrainment is presented. The influence of such admixtures on bond to anchored reinforcing bars, frost durability, mechanical properties, and rapid-chloride permeability is also highlighted. Special applications where such relatively new admixtures can significantly enhance performance are highlighted, including their incorporation in concrete intended for underwater placement and repair, self-consolidating and segregation-free concrete for abovewater construction, and structural grout for filling post-tensioning ducts.

Mass‐ and Density‐Dependent Reproductive Success and Reproductive Costs in a Capital Breeder

Abstract: For capital breeders, mass may affect reproductive potential. Reproductive expenditure may reduce future reproductive potential, particularly when resources are scarce. To test the hypothesis that reproductive success and the costs of reproduction vary according to mass and population density, we analyzed 25 yr of data on bighorn ewes (Ovis canadensis). The number of adult females was first limited by yearly removals, then allowed to triple. We found no survival costs of reproduction for ewes aged 4–7 yr. For ewes aged 8–14 yr, survival was density dependent for barren ewes but not for ewes that weaned lambs. Failure to lamb was rare and negatively correlated with fertility the following year. At low population density, lactation had a negative effect on mass gain but had a limited reproductive cost. At high density, heavy ewes had higher reproductive success than light ewes, and the reproductive cost and somatic costs of reproduction increased. The cost of reproduction was greater for light than...

Chemical admixture-cement interactions: Phenomenology and physico-chemical concepts

Abstract: Chemical admixtures are often used to alter the course of cement hydration reactions and the properties of fresh or hardened concrete. The admixtures, in most cases organic compounds, can perform such functions through various types of physico-chemical interactions with the hydrating cement phases. To understand the consequences of admixture-cement interactions, and to optimize the functional properties of admixtures, appropriate descriptions of their mode of action must be developed. An overview of the latter is attempted here, drawing mainly from: (1) the chemistry and phenomenology of cement hydration; (2) a review of the various types of molecular processes in which admixture molecules can be involved at the solid-solution interface; (3) selected experimental data on the influence of water reducers and superplasticizers which illustrate some basic features of admixture-cement interactions.

Planning for temporally extended goals

Abstract: In planning, goals have traditionally been viewed as specifying a set of desirable final states. Any plan that transforms the current state to one of these desirable states is viewed to be correct. Goals of this form are limited in what they can specify, and they also do not allow us to constrain the manner in which the plan achieves its objectives. We propose viewing goals as specifying desirable sequences of states, and a plan to be correct if its execution yields one of these desirable sequences. We present a logical language, a temporal logic, for specifying goals with this semantics. Our language is rich and allows the representation of a range of temporally extended goals, including classical goals, goals with temporal deadlines, quantified goals (with both universal and existential quantification), safety goals, and maintenance goals. Our formalism is simple and yet extends previous approaches in this area. We also present a planning algorithm that can generate correct plans for these goals. This algorithm has been implemented, and we provide some examples of the formalism at work. The end result is a planning system which can generate plans that satisfy a novel and useful set of conditions.

Design and description of CS-ACELP: a toll quality 8 kb/s speech coder

Abstract: This paper describes the 8 kb/s speech coding algorithm G.729 which has been standardized by ITU-T. The algorithm is based on a conjugate-structure algebraic CELP (CS-ACELP) coding technique and uses 10 ms speech frames. The codec delivers toll-quality speech (equivalent to 32 kb/s ADPCM) for most operating conditions. This paper describes the coder structure in detail and discusses the reasons behind certain design choices. A 16-b fixed-point version has been developed as part of Recommendation G.729 and a summary of the subjective test results based on a real-time implementation of this version are presented.

Effects of density and weather on survival of bighorn sheep lambs ( Ovis canadensis )

Abstract: Density-dependence in juvenile survival may be difficult to detect if survival is also affected by density-independent factors. We investigated the relationships among weather parameters, population density, and lamb survival of bighorn sheep with long-term data from a marked population where we manipulated population density. We distinguished neonatal survival and winter survival. Density interacted with weather variables to affect neonatal survival; spring and winter temperatures had a positive effect on neonatal survival only when population density was high. Neonatal survival was positively affected by spring precipitation independently of population density. Winter survival was positively correlated with temperature and precipitation during the previous spring, negatively correlated with density, and independent of winter temperature or snowfall. The effect of weather on lamb winter survival did not vary with density. Bighorn lambs are well adapted to harsh winter weather, but spring weather influenced survival of lambs at birth and during the subsequent winter, possibly through its effects on forage availability. Our study clearly demonstrates density-dependence in lamb survival. Some of the effects of weather on lamb survival are density-independent, others are mediated by an interaction with population density.

High performance concrete—An overview

Abstract: It is suggested that high performance concrete is not fundamentally different from the concrete used in the past, although it usually contains fly ash, ground granulated blastfurnace slag and silica fume, as well as superplasticizer. The cost aspects of the use of silica fume are considered. The content of cementitious material is high and the water/cement ratio is low; the maximum size of aggregate is small. Although ordinary Portland cement is used, it must be compatible with a given superplasticizer; the causes of incompatibility are discussed. The distinct shrinkage behaviour of high performance concrete is considered and the reasons for an absolute necessity of wet curing are given. Some uses of high performance concrete are mentioned. A ‘prediction’ of the future of high performance concrete and of concrete in general is offered.

Electron-nuclear dynamics of multiphoton H 2 + dissociative ionization in intense laser fields

Abstract: The time-dependent Schr\"odinger equation for the one-dimensional ${\mathrm{H}}_{2}^{+}$ molecule (with both nuclear and electronic degrees of freedom included) was solved numerically to study dissociative ionization. A wave-function splitting technique was used with projections onto Volkov states, which allows one to circumvent the problem of lost information on electron flux due to absorbing boundary methods. This technique allows us to calculate the above-threshold ionization (ATI) photo electron kinetic-energy spectra in the presence of moving nuclei, as well as complete spectra of dissociating protons, beyond the Born-Oppenheimer approximation. The ATI spectra are considerably enhanced with respect to the H-atom spectra due to electron molecule interaction. The peaks seen in calculated Coulomb explosion spectra of protons agree well with recent theoretical and experimental work related to the phenomenon of charge-resonance-enhanced ionization in molecules.

Accelerating the Convergence of Evolutionary Algorithms by Fitness Landscape Approximation

Abstract: A new algorithm is presented for accelerating the convergence of evolutionary optimization methods through a reduction in the number of fitness function calls Such a reduction is obtained by 1) creating an approximate model of the fitness landscape using kriging interpolation, and 2) using this model instead of the original fitness function for evaluating some of the next generations The main interest of the presented approach lies in problems for which the computational costs associated with fitness function evaluation is very high, such as in the case of most engineering design problems Numerical results presented for a test case show that the reconstruction algorithm can effectively reduces the number of fitness function calls for simple problems as well as for difficult multidimensional ones

Complications and Sequelae of Meningococcal Disease in Quebec, Canada, 1990–1994

Abstract: To study complications and sequelae of serogroup B and C meningococcal disease, a retrospective survey examined the outcome of all culture-proven cases reported in the province of Quebec, Canada, from January 1990 through December 1994 (serogroup B, 167 cases; serogroup C, 304 cases). Data were collected from medical files, postal questionnaires, and telephone interviews. Age groups having the most cases were the 10-19-year age group for serogroup C and the <1-year age group for serogroup B. Fatality rates were 7% for serogroup B and 14% for serogroup C disease. Only 3% of survivors of serogroup B disease had physical sequelae, compared with 15% of survivors of serogroup C disease (skin scars, 12%; amputations, 5%; hearing loss, 2%; renal problems, 1%; and other sequelae, 4%). These results confirm the gravity of disease caused by serogroup C, serotype 2a Neisseria meningitidis and justify liberal use of vaccination for outbreak control.

Electrokinetic Properties which Control the Coagulation of Silicate Cement Suspensions during Early Age Hydration

Abstract: The coagulation of cement particles during early age hydration has been previously identified as the first step of the setting and hardening of cement pastes. By hydrating Ca3SiO5and a silicate-rich clinker under controlled conditions, a correlation between the coagulation of the suspensions and the electrokinetic properties of particles is established. The zeta potential, and hence the surface charge, of particles in suspension depends on the calcium content of the medium. At low concentrations of Ca2+, the zeta potential of Ca3SiO5particles, calcium silicate hydrate (C–S–H), and clinker is negative (<−30 mV) and the suspensions are well dispersed. A strong coagulation occurs at intermediate concentrations (1 to 10 mmol/L of Ca2+) as the zeta potential becomes weaker under these conditions. The mean diameter of the aggregates decreases at high Ca2+concentrations, where the zeta potential of the particles is high and positive.

Investigation of depth-of-interaction by pulse shape discrimination in multicrystal detectors read out by avalanche photodiodes

Abstract: The measurement of depth of interaction (DOI) within detectors is necessary to improve resolution uniformity across the FOV of small diameter PET scanners. DOI encoding by pulse shape discrimination (PSD) has definite advantages as it requires only one readout per pixel and it allows DOI measurement of photoelectric and Compton events. The PSD time characteristics of various scintillators were studied with avalanche photodiodes (APD) and the identification capability was tested in multi-crystal assemblies with up to four scintillators. In the PSD time spectrum of an APD-GSO/LSO/BGO/CsI(Tl) assembly, four distinct time peaks at 45, 26, 88 and 150 ns relative to a fast test pulse, having resolution of 10.6, 5.2, 20 and 27 ns, can be easily separated. Whereas the number and position of scintillators in the multi-crystal assemblies affect detector performance, the ability to identify crystals is not compromised. Compton events have a significant effect on PSD accuracy, suggesting that photopeak energy gating should be used for better crystal identification. However, more sophisticated PSD techniques using parametric time-energy histograms can also improve crystal identification in cases where PSD time or energy discrimination alone is inadequate. These results confirm the feasibility of PSD DOI encoding with APD-based detectors for PET.

The acute and chronic effects of adrenocorticotropin on the levels of messenger ribonucleic acid and protein of steroidogenic enzymes in rat adrenal in vivo.

Abstract: The purpose of this study was to evaluate the effects of acute (a single injection) and chronic stimulation (twice daily injection for 9 days) by ACTH on changes occurring in the temporal expression of steroidogenic enzymes in the rat adrenal in vivo. Under acute ACTH stimulation, the level of steroidogenic acute regulatory protein (StAR) messenger RNA (mRNA) was increased within 0.5 h in both zona glomerulosa (ZG) and zona fasciculata-reticularis (ZFR), with maximal increases of 220-370% and 300-350% in the ZG and ZFR, respectively. Increases in the levels of StAR protein in homogenates were also found in the ZG (700%) and the ZFR (300%), but were delayed compared with those of their mRNA. Furthermore, the increase in mitochondrial StAR protein was concomitant with that in the homogenate, indicating that the entry of StAR into mitochondria might not be necessary to increase steroidogenesis during the early stimulatory phase. The levels of c-jun, c-fos, junB, and fosB mRNA in ZG and ZFR were also rapidly maximally elevated within 0.5-1 h after ACTH administration and fell to near control levels 5 h posttreatment. The levels of c-jun protein were already increased in both zones at 1 h, reached 200% at 3 h, and remained elevated 5 h post-ACTH treatment. The levels of c-Fos protein were maximally increased by 240% in both zones after 1 h and decreased thereafter to control values at 5 h. Few changes were observed in the adrenal protein contents of cholesterol side-chain cleavage cytochrome P450 (P450scc), cytochrome P450 11beta-hydroxylase (P450C11), cytochrome P450 21-hydroxylase (P450C21), and 3beta-hydroxysteroid dehydrogenase (3betaHSD). Under chronic stimulation by ACTH, we observed elevations in the levels of plasma corticosteroids and changes in the mRNA and protein levels of many adrenal steroidogenic enzymes in both zones. In the ZG, administration of ACTH for 9 days provoked an increase in the level of StAR mRNA (210-270%) and a decrease in the levels of 3betaHSD, cytochrome P450 aldosterone synthase (P450aldo), and AT1 receptor mRNA (by 40%, 70%, and 90%, respectively), whereas the levels of P450scc and P450C21 mRNA did not differ significantly from the control values. Western blotting analysis showed that the adrenal ZG protein levels of StAR and P450scc were increased (150%), 3betaHSD was not changed, and P450C21 was decreased by 70%. In the ZFR, the levels of P450scc and StAR mRNAs were increased (260% and 570-870%, respectively). The levels of 3betaHSD, P450C21, and P450C11 mRNA did not differ from control values in that zone. Western blotting analysis showed that the ZFR protein level of 3betaHSD was not changed, P450scc and P450C21 were decreased by 40% and 60%, respectively, and StAR was increased by 160%. Although c-fos and fosB mRNAs were undetectable after 9 days of chronic ACTH treatment, c-jun mRNA and its protein were still detectable, suggesting a basic role for this protooncogene in maintaining the integrity and function of the adrenal cortex. When dexamethasone was administered to rats for 5 days to inhibit their ACTH secretion, the mRNA levels of many steroidogenic enzymes were decreased, with the exception of StAR, 3betaHSD, and P450aldo. These results confirm the importance of physiological concentrations of ACTH in maintaining normal levels of adrenocortical enzymes and also indicate that in addition to ACTH, other factors are involved in controlling the expression of StAR, 3betaHSD, and P450aldo. In conclusion, we showed that ACTH acutely increases StAR mRNA followed, after a delay, by an increase in the level of StAR protein; this suggests that posttranslational modifications of the StAR precursor occurred during the early stimulatory phase and before the apparent translation of the newly formed mRNA. The rapid induction of protooncogenes suggests their participation in the action of ACTH to stimulate steroidogenesis. (ABSTRACT TRUNCATED)

Treatment of air polluted with high concentrations of toluene and xylene in a pilot‐scale biofilter

Abstract: Air biofiltration is now under active consideration for the removal of the volatile organic compounds from air polluted streams. In order to investigate the performance of this newly developed technology, a biofiltration pilot unit was operated for a continuous period of 8 months. The biofilter column was packed with commercially conditioned peat. At start-up, the filter bed was inoculated with four species of microorganisms. The resulting biofilter was fed with air contaminated with toluene, xylene or a mixture of toluene and xylene. The maximum elimination capacities attained were 165 g m−3 h−1 for toluene, 66 g m−3 h−1 for xylene and 115 g m−3 h−1 for the mixture of toluene and xylene. These specific performances exceed the values published in the technical and commercial literature for similar processes. Xylene isomers were degraded in decreasing order of reactivity, m-xylene, p-xylene, o-xylene. In the case of air polluted with a toluene and xylene mixture, it was noticed that the metabolism of toluene biodegradation was inhibited by the presence of xylene. Characterization of the biofilm microbial populations after several weeks of operation showed that the dominant strains among the isolated culturable strains from the biofilm, even if different from the initially inoculated strains, had at least one physiological property favoring degradation of aromatic organic rings. The performance of the biofilter was found to be dependent on the temperature of the filter media and the pressure drop through the bed. Finally, a steady state mathematical model was tested in order to theoretically describe the experimental results. This model is used to illustrate the operating diffusion and reaction regimes at steady state for the case of each pollutant. © 1998 Society of Chemical Industry

Depletion of yeast RNase III blocks correct U2 3′ end formation and results in polyadenylated but functional U2 snRNA

Abstract: Yeast U2 snRNA is transcribed by RNA polymerase II to generate a single non-polyadenylated transcript. A temperature-sensitive yeast strain carrying a disruption in RNT1, the gene encoding a homolog of RNase III, produces 3'-extended U2 that is polyadenylated. The U2 3'-flanking region contains a putative stem-loop that is recognized and cleaved at two sites by recombinant GST-Rnt1 protein in vitro. Removal of sequences comprising the stem-loop structure blocks cleavage in vitro and mimics the effects of Rnt1 depletion in vivo. Strains carrying a U2 gene lacking the Rnt1 cleavage site produce only polyadenylated U2 snRNA, and yet are not impaired in growth or splicing. The results suggest that eukaryotic RNase III may be a general factor in snRNA processing, and demonstrate that polyadenylation is not incompatible with snRNA function in yeast.

Steam reforming of naphthalene on Ni–Cr/Al2O3 catalysts doped with MgO, TiO2, and La2O3

Abstract: Magnesium, lanthanum, and titanium oxide-doped nickel–chromium/alumina catalysts were prepared and their performances assessed in the steam reforming of naphthalene (T: 1,023 K; water-to-naphthalene molar ratio: 16, atmospheric pressure, residence time of 0.55 s; and GHSV = 10,080 h−1). Impregnation of nickel and chromium in MgO- and La2O3-doped alumina gave the most active, stable catalysts. Nickel was active in aromatic ring opening, while chromium inhibited the encapsulation of the nickel clystallites by inactive carbon filaments. The use of MgO had a signifkant effect on the robustness of catalyst due to the formation of MgAl2O4 spinelphase. La2O3 was necessaly to drive the reaction toward total gasification. The addition of TiO2 gave poor activity and a soft catalyst. Some of the catalyst preparations were characterized before and after reaction by XRD, SEM, DTA, and surface measurements. The physical and catalytic properties are discussed in light of these characterizations. The catalyst optimized during this study showed high clystallite dispersion, excellent crushing strength, and good thermal stability. When comparing the data obtained using Ni/Al2O3 with Ni–Cr/Al203·MgO·La2O3, it is clear that the improved catalyst reached higher conversion and gas yield performances.

Detector response models for statistical iterative image reconstruction in high resolution PET

Abstract: One limitation in a practical implementation of statistical iterative image reconstruction is to compute a transition matrix accurately modeling the relationship between projection and image spaces. Detector response function (DRF) in positron emission tomography (PET) is broad and spatially-variant, leading to large transition matrices taking too much space to store. In this work, the authors investigate the effect of simpler DRF models on image quality in maximum likelihood expectation maximization reconstruction. The authors studied 6 cases of modeling projection/image relationship: tube/pixel geometric overlap with tubes centered on detector face; same as previous with tubes centered on DRF maximum; two different fixed-width Gaussian functions centered on DRF maximum weighing tube/pixel overlap; same as previous with a Gaussian of the same spectral resolution as DRF; analytic DRF based on linear attenuation of /spl gamma/-rays in detector arrays weighing tube/pixel overlap. The authors found that DRF oversimplification may affect visual image quality and image quantification dramatically, including artefact generation. They showed that analytic DRF yielded images of excellent quality for a small animal PET system with long, narrow detectors and generated a transition matrix for 2-D reconstruction that could be easily fitted into the memory of current stand-alone computers.

Hydroxyphthalocyanines as potential photodynamic agents for cancer therapy

Abstract: A series of benzyl-substituted phthalonitriles, substituted at the 3-, 4-, and 4,5-positions, underwent varied condensations with phthalonitrile to give a series of protected (monohydroxy- and polyhydroxyphthalocyaninato)zinc(II) derivatives which were readily cleaved to give several hydroxyphthalocyanines (ZnPc) (phthalocyanine phenol analogues). Their efficacy as sensitizers for the photodynamic therapy (PDT) of cancer was evaluated on the EMT-6 mammary tumor cell line. In vitro, the 2-hydroxy ZnPc (32) was the most active, followed by the 2,3- and 2,9-dihydroxy ZnPc (39 and 45), with the 2,9,16-trihydroxy ZnPc (33) exhibiting the least activity. In vivo, the monohydroxy derivative 32 and the 2,3-dihydroxy derivative 39 were both efficient in inducing tumor necrosis at 1 micromol kg-1, but complete tumor regression was poor, even at 2 micromol/kg. In contrast, the 2,9-dihydroxy isomer 45, at 2 micromol kg-1, induced tumor necrosis in all animals treated, with 75% complete regression. These results underline the importance of the position of the substituents on the Pc macrocycle to optimize tumor response and confirm the PDT potential of the unsymmetrical Pcs bearing functional groups on adjacent benzene rings.

Identification of a Pituitary Growth Hormone-Releasing Peptide (GHRP) Receptor Subtype by Photoaffinity Labeling

Abstract: Hexarelin, an analogue of GHRP-6, in which D-Tryptophan has been replaced by its 2-methyl derivative, is known to release growth hormone (GH) in vivo and in vitro by direct action on receptors present in anterior pituitary cells. Measurement of second messengers (c-AMP, Ca++, IP3) upon somatotrophs stimulation, suggests the existence of more than one GHRP receptor subtype. In order to document such an hypothesis, we have used a new photoactivatable derivative of Hexarelin, Tyr-Bpa-Ala-Hexarelin. This derivative was shown to be fully active in the release of GH in vivo with neonate rats. Using this photoactivatable ligand, we have specifically labeled a protein with an apparent Mr of 57 000 in human, bovine and porcine anterior pituitary membranes. Hexarelin and the spiroindoline sulfonamide MK-0677 displaced the Mr −57 000 photolabeled band with an apparent ED50 of 6×10−7 M and 2×10−5 M respectively. Taking into account the high efficiency (>60%) of covalent incorporation of the Bpa residue, this photoact...