Showing papers by "University of South Carolina published in 2003"

International physical activity questionnaire: 12-country reliability and validity

Abstract: CRAIG, C. L., A. L. MARSHALL, M. SJOSTROM, A. E. BAUMAN, M. L. BOOTH, B. E. AINSWORTH, M. PRATT, U. EKELUND, A. YNGVE, J. F. SALLIS, and P. OJA. International Physical Activity Questionnaire: 12-Country Reliability and Validity. Med. Sci. Sports Exerc., Vol. 35, No. 8, pp. 1381-1395, 2003. Background: Physical inactivity is a global concern, but diverse physical activity measures in use prevent international comparisons. The International Physical Activity Questionnaire (IPAQ) was developed as an instrument for cross-national monitoring of physical activity and inactivity. Methods: Between 1997 and 1998, an International Consensus Group developed four long and four short forms of the IPAQ instruments (administered by telephone interview or self-administration, with two alternate reference periods, either the "last 7 d" or a "usual week" of recalled physical activity). During 2000, 14 centers from 12 countries collected reliability and/or validity data on at least two of the eight IPAQ instruments. Test-retest repeatability was assessed within the same week. Concurrent (inter-method) validity was assessed at the same administration, and criterion IPAQ validity was assessed against the CSA (now MTI) accelerometer. Spearman's correlation coefficients are reported, based on the total reported physical activity. Results: Overall, the IPAQ questionnaires produced repeatable data (Spearman's clustered around 0.8), with comparable data from short and long forms. Criterion validity had a median of about 0.30, which was comparable to most other self-report validation studies. The "usual week" and "last 7 d" reference periods performed similarly, and the reliability of telephone administration was similar to the self-administered mode. Conclusions: The IPAQ instruments have acceptable measurement properties, at least as good as other established self-reports. Considering the diverse samples in this study, IPAQ has reasonable measurement properties for monitoring population levels of physical activity among 18- to 65-yr-old adults in diverse settings. The short IPAQ form "last 7 d recall" is recommended for national monitoring and the long form for research requiring more detailed assessment. Key Words: MEASUREMENT, SURVEILLANCE, EPIDEMIOLOGY

Social Vulnerability to Environmental Hazards

Abstract: Objective. County-level socioeconomic and demographic data were used to construct an index of social vulnerability to environmental hazards, called the Social Vulnerability Index (SoVI) for the United States based on 1990 data. Methods. Using a factor analytic approach, 42 variables were reduced to 11 independent factors that accounted for about 76 percent of the variance. These factors were placed in an additive model to compute a summary score—the Social Vulnerability Index. Results. There are some distinct spatial patterns in the SoVI, with the most vulnerable counties clustered in metropolitan counties in the east, south Texas, and the Mississippi Delta region. Conclusion. Those factors that contribute to the overall score often are different for each county, underscoring the interactive nature of social vulnerability—some components increase vulnerability; others moderate the effects.

Trends in Prevalence, Awareness, Treatment, and Control of Hypertension in the United States, 1988-2000

Abstract: ContextPrior analyses of National Health and Nutrition Examination Survey (NHANES) data through 1991 have suggested that hypertension prevalence is declining, but more recent self-reported rates of hypertension suggest that the rate is increasing.ObjectiveTo describe trends in the prevalence, awareness, treatment, and control of hypertension in the United States using NHANES data.Design, Setting, and ParticipantsSurvey using a stratified multistage probability sample of the civilian noninstitutionalized population. The most recent NHANES survey, conducted in 1999-2000 (n = 5448), was compared with the 2 phases of NHANES III conducted in 1988-1991 (n = 9901) and 1991-1994 (n = 9717). Individuals aged 18 years or older were included in this analysis.Main Outcome MeasuresHypertension, defined as a measured blood pressure of 140/90 mm Hg or greater or reported use of antihypertensive medications. Hypertension awareness and treatment were assessed with standardized questions. Hypertension control was defined as treatment with antihypertensive medication and a measured blood pressure of less than 140/90 mm Hg.ResultsIn 1999-2000, 28.7% of NHANES participants had hypertension, an increase of 3.7% (95% confidence interval [CI], 0%-8.3%) from 1988-1991. Hypertension prevalence was highest in non-Hispanic blacks (33.5%), increased with age (65.4% among those aged ≥60 years), and tended to be higher in women (30.1%). In a multiple regression analysis, increasing age, increasing body mass index, and non-Hispanic black race/ethnicity were independently associated with increased rates of hypertension. Overall, in 1999-2000, 68.9% were aware of their hypertension (nonsignificant decline of −0.3%; 95% CI, −4.2% to 3.6%), 58.4% were treated (increase of 6.0%; 95% CI, 1.2%-10.8%), and hypertension was controlled in 31.0% (increase of 6.4%; 95% CI, 1.6%-11.2%). Women, Mexican Americans, and those aged 60 years or older had significantly lower rates of control compared with men, younger individuals, and non-Hispanic whites.ConclusionsContrary to earlier reports, hypertension prevalence is increasing in the United States. Hypertension control rates, although improving, continue to be low. Programs targeting hypertension prevention and treatment are of utmost importance.

Academic Self-Concept and Self-Efficacy: How Different Are They Really?

Abstract: Academic motivation researchers sometimes struggle to decipher the distinctive characteristics of what appear to be highly analogous constructs. In this article, we discuss important similarities between self-concept and self-efficacy as well as some notable differences. Both constructs share many similarities such as centrality of perceived competence in construct definition; use of mastery experience, social comparison, and reflected appraisals as major information sources; and a domain-specific and multidimensional nature. Both predict motivation, emotion, and performance to varying degrees. However, there are also important differences. These differences include integration vs. separation of cognition and affect, heavily normative vs. goal-referenced evaluation of competence, aggregated vs. context-specific judgment, hierarchical vs. loosely hierarchical structure, past vs. future orientation, and relative temporal stability vs. malleability. We argue that self-efficacy acts as an active precursor of self-concept development and suggest that self-concept research separate out its multiple components and subprocesses and invest more effort toward making students less preoccupied with normative ability comparisons in school.

Violence and Risk of PTSD, Major Depression, Substance Abuse/Dependence, and Comorbidity: Results From the National Survey of Adolescents

Abstract: With a national household probability sample of 4,023 telephone-interviewed adolescents ages 12–17, this study provides prevalence, comorbidity, and risk-factor data for posttraumatic stress disorder (PTSD), major depressive episode (MDE), and substance abuse/dependence (SA/D). Roughly 16% of boys and 19% of girls met criteria for at least 1 diagnosis. Six-month PTSD prevalence was 3.7% for boys and 6.3% for girls, 6-month MDE prevalence was 7.4% for boys and 13.9% for girls, and 12-month SA/D prevalence was 8.2% for boys and 6.2% for girls. PTSD was more likely to be comorbid than were MDE and SA/D. Results generally support the hypothesis that exposure to interpersonal violence (i.e., physical assault, sexual assault, or witnessed violence) increases the risk of these disorders and of diagnostic comorbidity. Limited information exists about the prevalence and comorbidity of posttraumatic stress disorder (PTSD), major depressive episode (MDE), and substance abuse/dependence (SA/D) among probability samples of adolescents. The extent to which interpersonal violence increases risk of these disorders also remains understudied. We examined these issues using data from the National Survey of Adolescents (NSA). In addition to presenting national prevalence and comorbidity data for these three disorders, we tested the hypothesis that exposure to interpersonal violence increases risk of each disorder and of comorbidity. To date, the best estimates of these mental health problems and their comorbidity among younger age groups at the national level come from the National Comorbidity Survey (NCS; Kessler et al., 1994; Kessler, Sonnega, Bromet, Hughes, & Nelson, 1995; Kessler

Soil moisture retrieval from AMSR-E

Abstract: The Advanced Microwave Scanning Radiometer (AMSR-E) on the Earth Observing System (EOS) Aqua satellite was launched on May 4, 2002. The AMSR-E instrument provides a potentially improved soil moisture sensing capability over previous spaceborne radiometers such as the Scanning Multichannel Microwave Radiometer and Special Sensor Microwave/Imager due to its combination of low frequency and higher spatial resolution (approximately 60 km at 6.9 GHz). The AMSR-E soil moisture retrieval approach and its implementation are described in this paper. A postlaunch validation program is in progress that will provide evaluations of the retrieved soil moisture and enable improved hydrologic applications of the data. Key aspects of the validation program include assessments of the effects on retrieved soil moisture of variability in vegetation water content, surface temperature, and spatial heterogeneity. Examples of AMSR-E brightness temperature observations over land are shown from the first few months of instrument operation, indicating general features of global vegetation and soil moisture variability. The AMSR-E sensor calibration and extent of radio frequency interference are currently being assessed, to be followed by quantitative assessments of the soil moisture retrievals.

Predicting the use of web-based information systems: self-efficacy, enjoyment, learning goal orientation, and the technology acceptance model

Abstract: With the growing reliance on computerized systems and increasing rapidity of the introduction of new technologies, user acceptance of technology continues to be an important issue. Drawing upon recent findings in information systems, human computer interaction, and social psychology, the present research extends the technology acceptance model by incorporating the motivation variables of self-efficacy, enjoyment, and learning goal orientation in order to predict the use of Web-based information systems. One hundred nine subjects participated in the study, which was conducted in a field setting with the Blackboard system, a Web-based class management system. A survey was administered after a 2-week trial period and the actual use of the system was recorded by the Blackboard system over 8 weeks. The results largely support the proposed model, highlighting the important roles of self-efficacy, enjoyment, and learning goal orientation in determining the actual use of the system. Practical implications of the results are provided.

Adult chronic rhinosinusitis: Definitions, diagnosis, epidemiology, and pathophysiology

Abstract: Chronic rhinosinusitis Chronic rhinosinusitis (CRS) is a term that has been used to describe a number of entities characterized by chronic symptoms of nasal and sinus inflammation or infection. There has been a lack of consensus regarding definitions and treatments because CRS may be a spectrum of diseases with a range of appropriate treatments. The absence of widely accepted definitions for CRS has resulted in a paucity of research directed at understanding its pathophysiology and has hampered efforts to improve treatment. A Task Force was convened by the Sinus and Allergy Health Partnership to summarize much of the current and important information available regarding the prevalence, economic impact, pathophysiology, common inflammatory mediators, and the role of infectious microbes such as bacteria and fungi in CRS. The goal is to establish a standard and usable definition. Through this thorough review of the literature and the expert input from Task Force members, a definition was developed that serves to create a consistent baseline so that many of the currently debated or unanswered questions may be addressed. The new and more-specific Task Force definition is that “ Chronic rhinosinusitis is a group of disorders characterized by inflammation of the mucosa of the nose and paranasal sinuses of at least 12 weeks duration.” Recommended criteria for making the diagnosis of CRS for both clinical care and research were also outlined.

IAHS Decade on Predictions in Ungauged Basins (PUB), 2003–2012: Shaping an exciting future for the hydrological sciences

Abstract: Drainage basins in many parts of the world are ungauged or poorly gauged, and in some cases existing measurement networks are declining. The problem is compounded by the impacts of human-induced changes to the land surface and climate, occur-ring at the local, regional and global scales. Predictions of ungauged or poorly gauged basins under these conditions are highly uncertain. The IAHS Decade on Predictions in Ungauged Basins, or PUB, is a new initiative launched by the International Association of Hydrological Sciences (IAHS), aimed at formulating and implementing appropriate science programmes to engage and energize the scientific community, in a coordinated manner, towards achieving major advances in the capacity to make predictions in ungauged basins. The PUB scientific programme focuses on the estimation of predictive uncertainty, and its subsequent reduction, as its central theme. A general hydrological prediction system contains three components: (a) a model that describes the key processes of interest, (b) a set of parameters that represent those landscape properties that govern critical processes, and (c) appropriate meteorological inputs (where needed) that drive the basin response. Each of these three components of the prediction system, is either not known at all, or at best known imperfectly, due to the inherent multi-scale space-time heterogeneity of the hydrological system, especially in ungauged basins. PUB will therefore include a set of targeted scientific programmes that attempt to make inferences about climatic inputs, parameters and model structures from available but inadequate data and process knowledge, at the basin of interest and/or from other similar basins, with robust measures of the uncertainties involved, and their impacts on predictive uncertainty. Through generation of improved understanding, and methods for the efficient quantification of the underlying multi-scale heterogeneity of the basin and its response, PUB will inexorably lead to new, innovative methods for hydrological predictions in ungauged basins in different parts of the world, combined with significant reductions of predictive uncertainty. In this way, PUB will demonstrate the value of data, as well as provide the information needed to make predictions in ungauged basins, and assist in capacity building in the use of new technologies. This paper presents a summary of the science and implementation plan of PUB, with a call to the hydrological community to participate actively in the realization of these goals.

Groundwater and pore water inputs to the coastal zone

Abstract: Both terrestrial and marine forces drive underground fluid flows in the coastal zone. Hydraulic gradients on land result in groundwater seepage near shore and may contribute to flows further out on the shelf from confined aquifers. Marine processes such as tidal pumping and current-induced pressure gradients may induce interfacial fluid flow anywhere on the shelf where permeable sediments are present. The terrestrial and oceanic forces overlap spatially so measured fluid advection through coastal sediments may be a result of composite forcing. We thus define “submarine groundwater discharge” (SGD) as any and all flow of water on continental margins from the seabed to the coastal ocean, regardless of fluid composition or driving force. SGD is typically characterized by low specific flow rates that make detection and quantification difficult. However, because such flows occur over very large areas, the total flux is significant. Discharging fluids, whether derived from land or composed of re-circulated seawater, will react with sediment components. These reactions may increase substantially the concentrations of nutrients, carbon, and metals in the fluids. These fluids are thus a source of biogeochemically important constituents to the coastal ocean. Terrestrially-derived fluids represent a pathway for new material fluxes to the coastal zone. This may result in diffuse pollution in areas where contaminated groundwaters occur. This paper presents an historical context of SGD studies, defines the process in a form that is consistent with our current understanding of the driving forces as well as our assessment techniques, and reviews the estimated global fluxes and biogeochemical implications. We conclude that to fully characterize marine geochemical budgets, one must give due consideration to SGD. New methodologies, technologies, and modeling approaches are required to discriminate among the various forces that drive SGD and to evaluate these fluxes more precisely.

Life-long reduction in MnSOD activity results in increased DNA damage and higher incidence of cancer but does not accelerate aging

Abstract: Mice heterozygous for the Sod2 gene (Sod2+/− mice) have been used to study the phenotype of life-long reduced Mn-superoxide dismutase (MnSOD) activity. The Sod2+/− mice have reduced MnSOD activity ...

Social Capital in Multinational Corporations and a Micro-Macro Model of its Formation

Abstract: Linking theories of social capital with theories of multinational corporations (MNCs), we discuss the need for and creation of social capital in foreign subunits of MNCs. We argue that the required levels and forms of social capital are determined by the nature of interdependence between headquarters and subunits, and thus vary by different models of MNCs. We then develop a micro-macro model of social capital formation, explaining how boundary spanners form their private social capital and how this social capital is transformed into public social capital of the subunit.

Preferential End-to-End Assembly of Gold Nanorods by Biotin−Streptavidin Connectors

Abstract: A major challenge in nanoscience and nanotechnology is the rational assembly of nanoscale objects. Here we report that gold nanorods, aspect ratio 18, can be functionalized with a biotin disulfide, and subsequent addition of streptavidin links the rods together in an end-to-end manner much more often than expected.

Solution-phase synthesis of Cu2O nanocubes

Abstract: We report here the solution-phase synthesis of highly uniform and monodisperse cubic Cu2O nano- and microcubes. Copper(II) salts in water are reduced with sodium ascorbate in air, in the presence of a surfactant. The average edge length of the cubes varies from 200 to 450 nm, as a function of surfactant concentration. Transmission electronic microscopy suggests that these cubes are composed of small nanoparticles and appear to be hollow.

Dependence of the Gold Nanorod Aspect Ratio on the Nature of the Directing Surfactant in Aqueous Solution

Abstract: A three-step seed-mediated growth method was used to make gold nanoparticles. Different surfactants, alkyltrimethylammonium bromides (CnTAB, n = 10, 12, 14, 16, and 18) and cetylpyridinium chloride (C16PC), were chosen as stabilizers. In general, it was found that as the length of the surfactant chain increased, the resulting gold nanoparticles' aspect ratio increased: the aspect ratio was 1 (for C10TAB), 5 ± 2 (C12TAB), 17 ± 3 (C14TAB), and 23 ± 4 (C16TAB). The plasmon absorption maxima for the gold nanoparticles varied as a function of the shape, from 520 nm (spheres) to beyond 2000 nm (high aspect ratio nanorods). We propose that the surfactant binds as a bilayer to the growing nanoparticle and assists in nanoparticle elongation via a “zipping” mechanism.

Developing and Validating an Observational Learning Model of Computer Software Training and Skill Acquisition

Abstract: Computer skills are key to organizational performance, and past research indicates that behavior modeling is a highly effective form of computer skill training. The present research develops and tests a new theoretical model of the underlyingobservational learning processes by which modeling-based training interventions influence computer task performance. Observational learning processes are represented as a second-order construct with four dimensions (attention, retention, production, and motivation). New measures for these dimensions were developed and shown to have strong psychometric properties. The proposed model controls for two pretraining individual differences (motivation to learn and self-efficacy) and specifies the relationships among three training outcomes (declarative knowledge, post-training self-efficacy, and task performance). The model was tested using PLS on data from an experiment ( N = 95) on computer spreadsheet training. As hypothesized, observational learning processes significantly influenced training outcomes. A representative modeling-based training intervention (retention enhancement) significantly improved task performance through its specific effects on the retention processes dimension of observational learning. The new model provides a more complete theoretical account of the mechanisms by which modeling-based interventions affect training outcomes, which should enable future research to systematically evaluate the effectiveness of a wide range of modeling-based training interventions. Further, the new instruments can be used by practitioners to refine ongoing training programs.

Recreational physical activity and the risk of breast cancer in postmenopausal women: the women's health initiative cohort study.

Abstract: ContextWomen who are physically active have a decreased risk for breast cancer, but the types, amounts, and timing of activity needed are unknown.ObjectiveTo prospectively examine the association between current and past recreational physical activity and incidence of breast cancer in postmenopausal women.Design, Setting, and PatientsProspective cohort study in 74 171 women aged 50 to 79 years who were recruited by 40 US clinical centers from 1993 through 1998.Main Outcome MeasureIncident invasive and in situ breast cancer.ResultsWe documented 1780 newly diagnosed cases of breast cancer over a mean follow-up of 4.7 years. Compared with less active women, women who engaged in regular strenuous physical activity at age 35 years had a 14% decreased risk of breast cancer (relative risk [RR], 0.86; 95% confidence interval [CI], 0.78-0.95). Similar but attenuated findings were observed for strenuous physical activity at ages 18 years and 50 years. An increasing total current physical activity score was associated with a reduced risk for breast cancer (P = .03 for trend). Women who engaged in the equivalent of 1.25 to 2.5 hours per week of brisk walking had an 18% decreased risk of breast cancer (RR, 0.82; 95% CI, 0.68-0.97) compared with inactive women. Slightly greater reduction in risk was observed for women who engaged in the equivalent of 10 hours or more per week of brisk walking. The effect of exercise was most pronounced in women in the lowest tertile of body mass index (BMI) (<24.1), but also was observed for women in the middle tertile of BMI (24.1-28.4).ConclusionsThese data suggest that increased physical activity is associated with reduced risk for breast cancer in postmenopausal women, longer duration provides most benefit, and that such activity need not be strenuous.

Six Novel Missense Mutations in the LDL Receptor-Related Protein 5 (LRP5) Gene in Different Conditions with an Increased Bone Density

Abstract: Bone is a dynamic tissue that is subject to the balanced processes of bone formation and bone resorption. Imbalance can give rise to skeletal pathologies with increased bone density. In recent years, several genes underlying such sclerosing bone disorders have been identified. The LDL receptor-related protein 5 (LRP5) gene has been shown to be involved in both osteoporosis-pseudoglioma syndrome and the high–bone-mass phenotype and turned out to be an important regulator of peak bone mass in vertebrates. We performed mutation analysis of the LRP5 gene in 10 families or isolated patients with different conditions with an increased bone density, including endosteal hyperostosis, Van Buchem disease, autosomal dominant osteosclerosis, and osteopetrosis type I. Direct sequencing of the LRP5 gene revealed 19 sequence variants. Thirteen of these were confirmed as polymorphisms, but six novel missense mutations (D111Y, G171R, A214T, A214V, A242T, and T253I) are most likely disease causing. Like the previously reported mutation (G171V) that causes the high–bone-mass phenotype, all mutations are located in the aminoterminal part of the gene, before the first epidermal growth factor–like domain. These results indicate that, despite the different diagnoses that can be made, conditions with an increased bone density affecting mainly the cortices of the long bones and the skull are often caused by mutations in the LRP5 gene. Functional analysis of the effects of the various mutations will be of interest, to evaluate whether all the mutations give rise to the same pathogenic mechanism.

Preliminary Validation of the Brief Multidimensional Students' Life Satisfaction Scale (BMSLSS)

Abstract: Two studies investigated the psychometricproperties of the Brief MultidimensionalStudents' Life Satisfaction Scale (MSLSS: Huebner, 1994). In Study 1, 221 middle schoolstudents completed the Brief MultidimensionalStudents' Life Satisfaction Scale (BMSLSS),Multidimensional Students' Life SatisfactionScale, Students' Life Satisfaction Scale (SLSS:Huebner, 1991a), Positive and Negative AffectSchedule-Children's Version (Laurent et al.,1999), Children's Social Desirability Scale(Crandall et al., 1965), and a one-item globallife satisfaction rating (GLLS). Students alsorated the importance of the BMSLSS five lifesatisfaction domains (Family, Friends, School,Self, Living Environment). The results revealedacceptable internal consistency reliability,criterion-related validity, and constructvalidity for the BMSLSS Total score forresearch purposes. Furthermore, evidence ofconvergent and discriminant validity for theBMSLSS domain scores was also obtained throughmultitrait-multimethod analyses. Finally, theresults failed to provide strong support forthe usefulness of importance scores inpredicting overall life satisfaction;unweighted BMSLSS scores were highly related toglobal life satisfaction (GLLS) scores. InStudy 2, 46 high school students completed theBMSLSS and MSLSS to test the generalizabilityof the convergent and discriminant validityfindings with older adolescents. The findingsrevealed stronger evidence of validity withthis age group. Overall, the findings offered preliminary support for the reliability andvalidity of the BMSLSS, suggesting that it canserve as a useful alternative to the longerMSLSS in studies with adolescents in whichbrevity is an important consideration.

Impulsivity and rapid discounting of delayed hypothetical rewards in cocaine-dependent individuals.

Abstract: In this study, crack/cocaine-dependent (CD) and non-drug-using matched control (MC) participants were presented with hypothetical immediate and delayed rewards, with 16 delay conditions ranging from 5 min to 25 years. All participants were presented with hypothetical monetary rewards; however, the CD group was also presented with hypothetical crack/cocaine rewards. The objective value of the rewards ranged from $1 to $1,000. Hyperbolic discounting functions provided a good fit of the data. The CD group discounted monetary rewards at a higher rate than the MC group did, and the CD group discounted crack/cocaine rewards at a higher rate than it did monetary rewards. Moreover, scores on self-report measures indicated greater impulsivity in the CD group when compared with the MC group.

Maillard reaction products in tissue proteins: new products and new perspectives.

Abstract: The chemical modification of protein by nonenzymatic browning or Maillard reactions increases with age and in disease. Maillard products are formed by reactions of both carbohydrate- and lipid-derived intermediates with proteins, leading to formation of advanced glycation and lipoxidation end-products (AGE/ALEs). These modifications and other oxidative modifications of amino acids increase together in proteins and are indicators of tissue aging and pathology. In this review, we describe the major pathways and characteristic products of chemical modification of proteins by carbohydrates and lipids during the Maillard reactions and identify major intersections between these pathways. We also describe a new class of intracellular sulfhydryl modifications, Cys-AGE/ALEs, that may play an important role in regulatory biology and represent a primitive link between nonenzymatic and enzymatic chemistry in biological systems.

Observation of a Narrow Meson State Decaying to Ds+π0 at a Mass of 2.32 GeV/c2

Abstract: We have observed a narrow state near 2.32 GeV/c(2) in the inclusive D(+)(s)pi(0) invariant mass distribution from e(+)e(-) annihilation data at energies near 10.6 GeV. The observed width is consistent with the experimental resolution. The small intrinsic width and the quantum numbers of the final state indicate that the decay violates isospin conservation. The state has natural spin-parity and the low mass suggests a J(P)=0(+) assignment. The data sample corresponds to an integrated luminosity of 91 fb(-1) recorded by the BABAR detector at the SLAC PEP-II asymmetric-energy e(+)e(-) storage ring.

Integration from proteins to organs: the Physiome Project.

Abstract: The Physiome Project will provide a framework for modelling the human body, using computational methods that incorporate biochemical, biophysical and anatomical information on cells, tissues and organs. The main project goals are to use computational modelling to analyse integrative biological function and to provide a system for hypothesis testing.