Institution
Wroclaw University of Environmental and Life Sciences
Education•Wrocław, Poland•
About: Wroclaw University of Environmental and Life Sciences is a education organization based out in Wrocław, Poland. It is known for research contribution in the topics: Population & GNSS applications. The organization has 3108 authors who have published 6672 publications receiving 57774 citations.
Topics: Population, GNSS applications, Yarrowia, Soil water, Flavonols
Papers published on a yearly basis
Papers
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TL;DR: The results of the experiment suggested that the administration of polysaccharide B fraction from Caltha palustris extract significantly suppressed the progression of CIA, indicating that fraction B may be a potent candidate for botanical anti-arthritic agent.
25 citations
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TL;DR: Experiments on tumor-engrafted animals indicate substantial inhibition of tumor growth and biodistribution analysis in mice indicates prolonged circulation characteristic for PEG-modified liposomal carriers.
Abstract: The chemical nature of genetic drugs (e.g. antisense oligonucleotides, siRNA, vectors) requires a suitable carrier system to protect them from enzymatic degradation without changing their properties and enable efficient delivery into target cells. Lipid vectors for nucleic acid delivery that have been widely investigated for years can be very effective. As the majority of attempts made in the field of cancer gene therapy have focused on solid tumors, while blood cancer cells have attracted less attention, the latter became the subject of our investigation. The lipid carrier proposed here is based on liposomes constructed by others but the lipid composition is original. A liposome-coated lipoplex (L-cL) consists of a core arising from complexation of positively charged lipid and negatively charged oligodeoxynucleotide (ODN) or plasmid DNA coated by a neutral or anionic lipid bilayer. Moreover, our lipid vector demonstrates size stability and is able to retain a high content of enclosed plasmid DNA or antisense oligodeoxynucleotides (asODNs). Observed transfection efficacies of the tested preparation using a plasmid coding for fluorescent protein were up to 60-85% of examined leukemia cells (Jurkat T and HL-60 lines) in the absence or the presence of serum. When BCL‑2 asODN was encapsulated in the L-cL, specific silencing of this gene product at both the mRNA and protein level and also a markedly decreased cell survival rate were observed in vitro. Moreover, biodistribution analysis in mice indicates prolonged circulation characteristic for PEG-modified liposomal carriers. Experiments on tumor-engrafted animals indicate substantial inhibition of tumor growth.
25 citations
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TL;DR: Biotransformations of 6-methylflavone and the attachment of the sugar unit to the flavonoid substrate having no hydroxyl group are described and it is proved that I. fumosorosea KCH J2 is an effective biocatalyst for glycosylation of flavonoids compounds.
Abstract: Entomopathogenic fungi are known for their ability to carry out glycosylation of flavonoids, which usually results in the improvement of their stability and bioavailability. In this study we used a newly isolated strain of the entomopathogenic filamentous fungus Isaria fumosorosea KCH J2 as a biocatalyst. Our aim was to evaluate its ability to carry out the biotransformation of flavonoids and to obtain new flavonoid derivatives. The fungus was isolated from a spider's carcass and molecularly identified using analysis of the ITS1-ITS2 rDNA sequence. As a result of biotransformation of 6-methylflavone two new products were obtained: 6-methylflavone 8-O-β-D-(4"-O-methyl)-glucopyranoside and 6-methylflavone 4'-O-β-D-(4"-O-methyl)-glucopyranoside. Chemical structures of the products were determined based on spectroscopic methods (1H NMR, 13C NMR, COSY, HMBC, HSQC). Our research allowed us to discover a new species of filamentous fungus capable of carrying out glycosylation reactions and proved that I. fumosorosea KCH J2 is an effective biocatalyst for glycosylation of flavonoid compounds. For the first time we describe biotransformations of 6-methylflavone and the attachment of the sugar unit to the flavonoid substrate having no hydroxyl group. The possibility of using flavonoid aglycones is often limited by their low bioavailability due to poor solubility in water. The incorporation of a sugar unit improves the physical properties of tested compounds and thus increases the chance of using them as pharmaceuticals.
25 citations
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TL;DR: The results show that the invasive Solidago species had similar competitive abilities to native species, and the necessity to monitor this species as a potentially invasive taxon is suggested.
25 citations
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TL;DR: This study established a method to alter the quantity and FA composition of SCO by overexpression of the glycerol phosphate acyltransferase gene (SCT1), which showed a 10-fold improvement in linoleic acid synthesis (C18:2) in a shake-flask experiment.
25 citations
Authors
Showing all 3137 results
Name | H-index | Papers | Citations |
---|---|---|---|
Jan Oszmiański | 47 | 204 | 8514 |
Aneta Wojdyło | 40 | 182 | 6832 |
Francesco Sansone | 37 | 135 | 4977 |
Guido Viscardi | 35 | 176 | 3832 |
Jan Szopa | 33 | 169 | 4054 |
Henryk Okarma | 33 | 65 | 3264 |
Gaetano Donofrio | 32 | 141 | 3882 |
Andrzej Zalewski | 31 | 85 | 2372 |
Adam Figiel | 30 | 94 | 3309 |
Krzysztof Marycz | 30 | 197 | 3121 |
Waldemar Rymowicz | 29 | 91 | 2560 |
Pierluigi Quagliotto | 28 | 93 | 2330 |
Alfonso Moriana | 28 | 84 | 2489 |
Joost van Hoof | 26 | 95 | 2964 |
Nadia Barbero | 25 | 85 | 1642 |