Wroclaw University of Environmental and Life Sciences

About: Wroclaw University of Environmental and Life Sciences is a education organization based out in Wrocław, Poland. It is known for research contribution in the topics: Population & GNSS applications. The organization has 3108 authors who have published 6672 publications receiving 57774 citations.

THE EFFECT OF NITROGEN FERTILIZATION AND IRRIGATION ON YIELDING AND NUTRITIONAL STATUS OF SWEET BASIL (Ocimum basilicum L.)

Abstract: Sweet basil (Ocimum basilicum L.) is an annual plant belonging to the plant family Lamiaceae. There are many varieties of basil, with leaves of colour from green to purple and with small white or pink flower. In field experiment conducted in the years 2007-2008 there was investigated the effect of nitrogen fertilization in the doses of 50, 150 and 250 kg N·ha -1 and irrigation on yielding and nutritional status of basil of 'Red Rubin' cultivar grown from transplants. Basil positively responded to irrigation and the increase in its yield under the influence of irrigation amounted from 97.37% to 25.36% in subsequent years of cultivation. Also the dose of nitrogen did advantageously affect basil herb yield. The highest yields were recorded at the dose of 150-250 kg N·ha -1 . The high- est amount of nitrates ranged from 697.00 to 938.00 mg·kg -1 f.m. was determined in plants fertilized with nitrogen dose of 250 kg N·ha -1 . The level of macroelements de- pended both on irrigation and on nitrogen doses. Irrigation caused decreased amounts of phosphorus, potassium and calcium, while content of magnesium increased. The highest quantities of phosphorus and magnesium were obtained at nitrogen dose of 150 kg N·ha -1 , while for the highest values of potassium and calcium nitrogen dose was 250 kg N·ha -1 .

Copper(I) iodide complexes containing new aliphatic aminophosphine ligands and diimines—luminescent properties and antibacterial activity

Abstract: The new, water soluble, aminomethylphosphines were synthesized from P(CH2OH)3 and alkylpiperazines: P(CH2N(CH2CH2)2NCH3)3 (1) and P(CH2N(CH2CH2)2NCH2CH3)3 (2). Described already in literature P(CH2N(CH2CH2)2O)3 (3) were also obtained. The spectroscopic 1H, 31P and 13C NMR analyses and crystallographic studies of 1, 2 and 3 demonstrate that all these compounds have similar structures and spectroscopic properties, which almost do not depend on aliphatic rings in the molecules. Heteroleptic copper(I) iodide complexes with phosphines mentioned above and 2,2′-bipyridine (bpy): [CuI(bpy)P(CH2N(CH2CH2)2NCH3)3] (1B), [CuI(bpy)P(CH2N(CH2CH2)2NCH2CH3)3] (2B), [CuI(bpy)P(CH2N(CH2CH2)2O)3] (3B) or 1,10-phenanthroline (phen): [CuI(phen)P(CH2N(CH2CH2)2NCH3)3] (1P), [CuI(phen)P(CH2N(CH2CH2)NCH2CH3)3] (2P), [CuI(phen)P(CH2N(CH2CH2)2O)3] (3P) were also synthesized. All complexes were characterized by 1H, 31P and 13C NMR spectroscopy also. Molecular structures of 1P·PhCH3 and 3P·0.5Cu2I2(phen)2 were determined from single crystal X-ray diffraction studies. Upon excitation at 470 nm, all complexes in the solid state exhibit red photoluminescence due to charge transfer transition. The luminescence of phen complexes is higher than the luminescence of bpy ones. The presented phosphines and copper(I) complexes were screened for their in vitro antibacterial and antifungal activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus strains and Candida albicans. All the copper complexes exhibit significant antibacterial activity against Staphylococcus aureus strains. The activity of 1,10-phenanthroline complexes is higher than 2,2′-bipyridine complexes.

Ceramide galactosyltransferase (UGT8) is a molecular marker of breast cancer malignancy and lung metastases

Abstract: It was shown recently on the level of gene expression that UGT8, coding UDP-galactose:ceramide galactosyltransferase, is one of six genes whose elevated expression correlated with a significantly increased the risk of lung metastases in breast cancer patients. In this study primary tumours and their lung metastases as well as breast cancer cell lines were analysed for UGT8 expression at the protein level. Expression of UGT8 in breast cancer tissue specimens and breast cancer cell lines was analysed using IHC, real-time PCR and Western blotting. Comparison of the average values of the reaction intensities (IRS scale) showed a significant difference in UGT8 expression between (1) primary and metastatic tumours (Mann–Whitney U, P<0.05), (2) tumours of malignancy grades G3 and G2 (Mann–Whitney U, P<0.01) as well as G3 and G1 (Mann–Whitney U, P<0.001) and (3) node-positive and node-negative tumours (Mann–Whitney U, P<0.001). The predictive ability of increased expression of UGT8 was validated at the mRNA level in three independent cohorts of breast cancer patients (721). Similarly, breast cancer cell lines with the ‘luminal epithelial-like’ phenotype did not express or weakly expressed UGT8, in contrast to malignant, ‘mesenchymal-like,’ cells forming metastases in nude mice. Our data suggest that UGT8 is a significant index of tumour aggressiveness and a potential marker for the prognostic evaluation of lung metastases in breast cancer.