Showing papers in "Journal of Investigative Dermatology in 1999"

TL;DR: A small, portable, and robust confocal microscope that is capable of imaging normal and abnormal skin morphology and dynamic processes in vivo, in both laboratory and clinical settings is built.

TL;DR: The aims of this review are to highlight the recent progresses of knowledge on the organization and assembly of hemidesmosomes, their involvement in signaling pathways as well as their participation in clinical pathologic conditions.

TL;DR: This classification aid serves as a basic introduction into the field ofhair follicle morphogenesis, aims at standardizing the presentation of related hair research data, and should become a useful tool when screening new mouse mutants for discrete abnormalities of hair follicles morphogenesis in a highly reproducible, easily applicable, and quantifiable manner.

TL;DR: It is shown that in normal human epidermis, in hair follicles, and in stratified epidermal cultures, p63 protein is principally restricted to cells with high proliferative potential and is absent from the cells that are undergoing terminal differentiation.

TL;DR: In this paper, the ability of lesional and peripheral blood T cells to produce intracellular interferon-γ, tumor necrosis factor-α, interleukin-2 and interleucin-10 proteins was measured by flow cytometric analysis.

TL;DR: The results suggest that dermal sclerosis induced by BLM closely resembles systemic sclerosis both histologically and biochemically.

TL;DR: Assessment of apoptotic index by in situ end-labeling revealed extremely low rates of apoptosis in most malignant melanomas, and data demonstrate that apoptosis inhibition by survivin may participate in the onset and progression of malign melanoma, and suggest that therapeutic targeting of Survivin may be beneficial in patients with recurrent or metastatic disease.

TL;DR: It is demonstrated that primary human skin fibroblasts express Smads, a novel family of signaling molecules, in vitro in the absence of TGF-beta, and that Smad 7 may be involved in autocrine negative feedback in the regulation of COL1A2 promoter activity by T GF-beta.

TL;DR: This immortal human keratinocyte line HaCaT is frequently used as a paradigm for skin keratinocytes in vitro because of its highly preserved differentiation capacity and qualifies this cell line as an appropriate model for elucidation of the molecular mechanisms regulating Keratinocyte growth and differentiation and for use in pharmacotoxicology.

TL;DR: Clear differences in the location and frequencies of chromosomal aberrations in Spitz nevi and primary cutaneous melanomas could represent a basis for developing adjunctive techniques for refining accuracy in the difficult differential diagnosis of spitzoid melanocytic neoplasms.

TL;DR: SZ95 is an immortalized human facial sebaceous gland cell line that shows the morphologic, phenotypic and functional character-istics of normal human sebocytes.

TL;DR: The results suggest that Tβ4 is a potent wound healing factor with multiple activities that may be useful in the clinic.

TL;DR: Three-dimensional reconstruction of dorsal underfur follicles from serial sections made 14 mo after complete labeling of epidermis and hair follicles in neonatal mice disclosed the presence of highly persistent label-retaining cells associated with the first-generation follicle involved in the production of the first wave of hairs, commonly called the bulge.

TL;DR: Reactive oxygen species are important multifunctional mediators of ultraviolet B-induced ERK 1/2 and p38 signaling transduction pathways and suggest that ERK1/2 may play an important part in protecting keratinocytes from cell death following oxidative stress.

TL;DR: The data demonstrate that the presence of a functionally active iNOS is a crucial prerequisite for normal wound reepithelialization, and is further supported by the observation that proliferation of the keratinocyte cell line (HaCaT) is stimulated by low concentrations of NO-donors also in vitro.

TL;DR: It is demonstrated that a defined medium (supplemented keratinocyte defined medium) supports epidermal morphogenesis in organotypic cocultures equally well as serum-containing medium (mixture of Ham's F12 and Dulbecco's modified Eagle's medium), as documented by hallmarks of the epidersmal phenotype studied by immunofluorescence and electron microscopy.

TL;DR: The enhanced local production of eotaxin may lead to the recruitment of eosinophils and T lymphocytes, which both express CCR3 and contribute to the initiation and maintenance of inflammation.

TL;DR: A stereologic study on 235 hair follicles from different sites implies that the increase in the volume of the dermal papilla in these follicles is due to an increase inThe number of cells, either through proliferation or through the migration of cells from the follicular dermal sheath, and to an increases in the amount of extracellular matrix per cell.

TL;DR: The collective results show that UVA1 radiation primarily mediates singlet-oxygen damage triggering immediate pre-programmed cell death apoptosis (T < 20 min) by immediately opening the cyclosporine A-sensitive ("S" site) mitochondrial megapore, while superoxide anions initiate another cyclospora A-insensitive ("P" site") final apoptotic pathway.

TL;DR: Sebaceous gland secretion is a relevant physiologic pathway for the delivery of vitamin E to upper layers of facial skin and this mechanism may serve to protect skin surface lipids and the upper stratum corneum from harmful oxidation.

TL;DR: The features decreased growth related to ulcer age, altered morphology, and reduced response to platelet-derived growth factor are indicated, indicating that fibroblasts in some chronic wounds have approached or even reached the end of their lifespan (phase III).

TL;DR: The results strongly suggest the involvement of reactive oxygen species in arsenic-induced human skin cancer.

TL;DR: Although high numbers of T cells are present in lesional skin of both types, a lack of interleukin-13-induced B cell activation and consequent immunoglobulin E production in nonallergic atopic dermatitis is suggested.

TL;DR: It is concluded that K15 expression is not compatible with keratinocyte activation and the K15 gene is downregulated to maintain the activated phenotype.

TL;DR: A putative linkage to chromosome 1cen-q21 markers is disclosed, and the highest two-point lod score value was obtained with D1S305 marker, and non-parametric analysis at this locus demonstrated a significant excess of allele sharing.

TL;DR: Patients with dermatitis herpetiformis have elevated immunoglobulin A autoantibodies to tissue transglutaminase, confirming its pathogenic relation with celiac disease and further supporting the usefulness of this novel assay for screening and therapy control.

TL;DR: The results show that clusterin gene expression is induced in response to heat shock and oxidative stress in human A431 cells, and confers cellular protection against heat shockand oxidative stress.

TL;DR: This study including gas chromatography and mass spectrometry analysis indicates that some of the photoactive organic species commonly used today in sunscreens are unstable following ultraviolet irradiation.

TL;DR: These studies show that water can directly disrupt the barrier lipids and are consistent with surfactant-induced intercellular lamellar bilayer disruption being due at least in part to the deleterious action of water.

TL;DR: It is concluded that the lipids in human stratum corneum are highly ordered throughout the stratum Corneum and that electron diffraction allows monitoring of the local lipid organization, which contributes to the understanding of stratumCorneum barrier function.