Showing papers in "Journal of Pharmacological and Toxicological Methods in 2014"

TL;DR: Two in vitro cytotoxicity assays, new (PB) and classic (MTT) are compared in the assessment of viability of human umbilical vein endothelial cells (HUVECs) in the presence of selected plant extracts and fluorometric PrestoBlue assay is recommended.

TL;DR: The increasing amounts of data for defined toxicology endpoints have enabled machine learning models that have been increasingly used for predictions, and it is shown that across many different models Bayesian and SVM perform similarly based on cross validation data.

TL;DR: This study provides a proof-of-principle for the prediction of human TQT study results using data available early in drug development, and suggests that such approaches will provide a useful tool in cardiac safety assessment.

TL;DR: The ability of AP measured from hiPSC-CM to record the interactions of various ion channels via AP recording and avoid the limitations of using several single ion channel assays in a noncardiac tissue is shown.

TL;DR: Although C57BL/6J mice could not be a sensitive model to study anxiolytic effects in pharmacological or behavioral interventions, it might be a suitable model to test anxiogenic effects.

TL;DR: The current results provide the first unambiguous evidence that all preclinical in vivo repolarization assays, when accurately modeled and evaluated, yield results that are consistent with the conservation of moxifloxacin-induced QT prolongation across all common preclinical species.

TL;DR: The utility of MDCK-MDR1 permeability measurements was demonstrated in probing the role of P-glycoprotein transport in Primaquine-Chloroquine drug-drug interactions in comparison with in vivo pharmacokinetic changes and the utility of cell-based permeability assays in anti-malarial drug screening/development in regard to understanding transporter mediated changes in drug absorption/distribution.

TL;DR: This study confirms the suitability of the VMR assay as an efficient and predictive pre-clinical approach to evaluate adverse ocular effects of drugs on visual function in vivo.

TL;DR: Telemetry video-EEG for seizure liability investigations was characterized in three species, and yohimbine (SC and IV) and phenobarbital (IP) confirmed to be reliable positive controls as pro- and anticonvulsants, respectively.

TL;DR: A novel larval zebrafish model capable of simultaneous quantification of chronotropic, inotropic and arrhythmic effects is developed, potentially suitable for higher throughput drug safety and efficacy applications, pending further assessment of ZF-mammalian pharmacological comparability.

TL;DR: These results support and extend the limited evidence that Δ(9)-THC produces qualitatively different effects on behaviour depending upon the route of administration and show the potential for the vapourization procedure to produce a robust conditioned place preference (CPP) or conditioned place avoidance CPA.

TL;DR: ADRA was used to quantify NAC/NAL at 281nm, which showed high accuracy for the prediction of skin sensitization, similar to that of DPRA, and could be used to expand the range of chemicals tested in skin sensitized analyses.

TL;DR: The enlargement, hypertrophy-characteristic and potassium ion channels gene expression of hiPSC-derived cardiomyocytes suggest that low-density plating was sufficient to induce cardiachypertrophy.

TL;DR: Vapourized Δ(9)-THC administration produces behavioural effects qualitatively different from those induced by IP administration in rodents, and may produce behavioural effects more comparable to those observed in humans.

TL;DR: In this paper, the authors have provided updated information on the rationale, the applications, the advantages and limitations of common methods for screening drug effects on intracellular reactive oxygen species (ROS) linked to cell cycle study.

TL;DR: This procedure was effectively utilized for studies of the species-dependence of plasma protein and lipoprotein binding of three pyrethroids for inclusion in physiologically-based pharmacokinetic models of pyre Throids for use in health risk assessments of the insecticides in children and adults.

TL;DR: In this paper, a range of pharmacokinetic/pharmacodynamic (PK/PD) models are discussed that successfully described cardiovascular safety findings in the preclinical setting and where clinical data were available, it was found that translational modeling enabled the accurate prediction of outcomes in man and facilitated the description of the therapeutic index.

TL;DR: The Safety Pharmacology toolbox for detecting possible 5-HT2B receptor agonism liabilities of candidate drugs requires meticulous optimization and validation of all its components to perfect its human predictability power.

TL;DR: The newly developed 7-day Caco-2 model displayed comparable cellular morphology and integrity with the traditional 21-day model, which provides a rapid and economical option for assessing the drug permeability, and is applicable to the studies of intestinal drug absorption.

TL;DR: The findings indicate that the reagent can itself interact with the media causing significantly different fluorescence over time in a manner independent from the effect of intracellular reducing environment of living cells on alamar blue.

TL;DR: Results support that EEG, EOG and EMG monitoring by telemetry in Cynomolgus monkeys represents a useful non-clinical model to investigate and quantify drug-induced sleep disturbances.

TL;DR: The murine model of the SS is a valid tool for preclinical studies to screen drugs and drug combinations for the risk to cause an SS in man and the5-HT2A receptor has more impact on the 5-HT syndrome than previously suggested.

TL;DR: In this article, the effects of mdivi-1 on doxorubicin-induced cardiac dysfunction in naive and stressed conditions using Langendorff perfused heart models and a model of oxidative stress was used to assess the effect of drug treatments on the mitochondrial depolarisation and hypercontracture of cardiac myocytes.

TL;DR: Comparison of two widely used multiplex platforms for the detection of renal toxicity biomarkers in urine revealed the expected increases of urinary biomarkers after Cp treatment with similar results at the fold change level enabling consistent detection of kidney injury.

TL;DR: The present assay yeasts may be valuable tools for subtype-specific assessments of unidentified environmental ligand chemicals and receptor-specific pharmaceuticals.

TL;DR: The combination of in vitro hERG studies and in vivo studies in rat and monkey are consistent with no direct actions by ASOs on cardiac cell function or electrical conduction at relevant concentrations and dose levels.

TL;DR: Three models for permeability were successfully practiced in the study of puerarin absorption and the research strategy will be useful for herbal constituent absorption in the future.

TL;DR: A simple, inexpensive and reliable method to fabricate monopolar and bipolar electrodes, along with the swivel system, for delivery of electric pulses at the targeted sites in the brain of rat, suggesting that the electrodes, fabricated using this method, may serve as an economical and reliable tool in neuropharmacological and neurobehavioral studies.

TL;DR: It was showed that ractopamine residues persist in hair in 24days of withdrawal and also showed the possibility to test ractipamine from pig hair samples.

TL;DR: Light is shed on the pharmacokinetic properties of D-JNKI1 and supports its powerful neuroprotective effect in in vitro and in vivo experimental models.