Journal ArticleDOI
Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cells
TLDR
Two in vitro cytotoxicity assays, new (PB) and classic (MTT) are compared in the assessment of viability of human umbilical vein endothelial cells (HUVECs) in the presence of selected plant extracts and fluorometric PrestoBlue assay is recommended.About:
This article is published in Journal of Pharmacological and Toxicological Methods.The article was published on 2014-01-01. It has received 178 citations till now. The article focuses on the topics: MTT assay & Viability assay.read more
Citations
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Journal ArticleDOI
Use of the viability reagent PrestoBlue in comparison with alamarBlue and MTT to assess the viability of human corneal epithelial cells.
TL;DR: In this article, the authors compared PrestoBlue, alamarBlue, and 3-[4,5-dimethylthiazol-2-yl]-2,5diphenyl tetrazonium bromide (MTT) in assessing cell viability of human corneal epithelial cells (HCEC).
Journal ArticleDOI
A Comparative Review on the Extraction, Antioxidant Content and Antioxidant Potential of Different Parts of Walnut (Juglans regia L.) Fruit and Tree.
TL;DR: The antioxidant content of phenolic extracts obtained from different parts of the walnut tree and fruit are described to describe the antioxidant and antiradical activities of the prepared extracts.
Journal ArticleDOI
Zebrafish as an Emerging Model Organism to Study Angiogenesis in Development and Regeneration.
TL;DR: This review addresses and compares the most commonly used experimental models to study angiogenesis in vitro and in vivo and focuses on the implementation of the zebrafish (Danio rerio) as a model to studyAngiogenesis and discusses the advantages and not yet explored possibilities of its use as model organism.
Journal Article
Use of the Viability Reagent PrestoBlue in Comparison with AlamarBlue and MTT to Assess the Health of Human Corneal Epithelial Cells
TL;DR: PrestoBlue is more sensitive than MTT, but similar to alamarBlue in evaluating the viability of HCEC, and all the three plate colors and two reading modes showed similar results in the PrestoBlue assay in assessing the toxicity of SDS.
Book ChapterDOI
Cytotoxicity of African Medicinal Plants Against Normal Animal and Human Cells
TL;DR: The selectivity index is an important measure to identify substances with promising biological activity and negligible cytotoxicity, and extracting solvents ranging in polarity have been used to extract different plant parts, contributing to the wide variety of cytot toxicity results of African plants.
References
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Journal ArticleDOI
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Journal ArticleDOI
The phenolic constituents of Prunus domestica. I.—The quantitative analysis of phenolic constituents
T. Swain,W. E. Hillis +1 more
TL;DR: In this paper, methods for quantitative analysis of anthocyanins, leuco-anthocyanin, flavanols, and total phenols in plant tissue extracts are described.
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Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill
TL;DR: It was shown that phenol red does not interfere with the measurements and no washing steps are required since all ingredients can be added subsequently, and Serum proteins at concentrations up to 25% have no influence on the result.
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Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity
TL;DR: The identity of Alamar Blue is shown as resazurin, a very simple and versatile way of measuring cell proliferation and cytotoxicity that presents numerous advantages over other cytot toxicity or proliferation tests but there are several drawbacks to the routine use.
Journal ArticleDOI
A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay
TL;DR: The pattern of concanavalin A (ConA) or anti-CD3 antibody-induced proliferative response of murine lymphocytes as assessed by Alamar Blue was similar to that of a [3H]thymidine assay, which allows daily monitoring of proliferation without compromising the sterility of cultures.