Showing papers in "Journal of Proteomics in 2020"

TL;DR: This review, collects the pioneering and milestones works so far performed in the field of dairy microbiota, and provides the basic guidance to whom approaching the cheese microbiota investigation by means of the latest omics technologies.

TL;DR: This work implemented a robust package for retrieving and visualizing information from multiple sources such UniProtKB, SWISS-MODEL, and STRING, and will aid in saving time for downstream data analysis instead of manual time consuming data analysis.

TL;DR: Findings suggest that intermittent fasting from dawn to sunset for 30 consecutive days can be a preventive and adjunct therapy in cancer, metabolic syndrome, and several cognitive and neuropsychiatric diseases.

TL;DR: Here, in order to identify biomarker candidates in serum of TNBC patients, these nanomaterials were combined with electrophoretic separation (SDS-PAGE) to performed qualitative and quantitative comparisons of the serum proteomes of T NBC patients and healthy controls by liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis.

TL;DR: Different research methods to study the gut microbiota are introduced, with a specific focus on the current progress and application of metaproteomics, which reveals the taxonomy and functionality of the Gut microbiota, especially the functional pathways associated with diseases.

TL;DR: The antibacterial inhibitory activity of EO was preferentially associated with the presence of thymol and there was interference in protein regulation as well as DNA synthesis affected by these compounds, which contributed to these bioactive compounds being effectively used as agents in the safety and shelf life of food.

TL;DR: The latest proteomics based-studies on allergenic proteins are reviewed to provide guidance on the different MS-based methodologies adopted in the research on food allergens and the most common drawbacks encountered in a proteomic study are discussed, providing an overview that helps novel researchers in choosing the more suitable experimental workflow.

TL;DR: "native top-down MS", an approach based on Fourier Transform MS, whereby non-covalent interactions are preserved while covalent bonds are selectively fragmented are described.

TL;DR: KRT17 upregulation was confirmed in ESCC and its relationship with clinicopathological features were analysed, suggesting that KRT17 is significantly related to malignant progression and poor prognosis of ESCC patients, and it may serve as a new biological target for ESCC therapy.

TL;DR: Using the integrated transcriptome-assisted TMT-labeled quantitative proteomic approach to characterize changes in the proteomic profile of the longissimus dorsi muscle, differentially abundant proteins are identified that are associated with intramuscular fat deposition and fat biosynthesis in pigs.

TL;DR: This innovative proteomic approach applied on plasma samples allowed to identify circulating candidate biomarkers for beef tenderness, and three of them were identified as discriminative of tenderness both in Longissimus muscle and in plasma.

TL;DR: This study comprehensively evaluated commonly-used label-free quantification methods with a focus on missing values, precision, accuracy, selectivity, and reproducibility for low abundance protein quantification and showed that, among the strategies evaluated, the PD strategy with SEQUEST as a search engine performed better in terms of low abundanceprotein coverage.

TL;DR: It is reported that cryopreservation impacts numerous proteins involved in metabolism regulation (mainly mitochondria involved in the TCA cycle, and oxidative phosphorylation) and also affect proteins with oxidoreductase activity.

TL;DR: The data support the notion that saliva may be more suitable for the protein biomarker-based detection of oral cancer, and the newly developed SISCAPA-MRM assay could be applied to verify multiple oral cancer biomarker candidates in saliva samples.

TL;DR: Results obtained suggest a potential beneficial effect of TRP supplementation in terms of immune stimulation, without compromising growth or feed intake, and the incorporation of crystalized amino acids such as TRP into diets aims global better performance both at fish health/immune condition and growth development.

TL;DR: This study improves the assessment of sProtein detection and enables their functional characterization in future experiments, and further describes properties and potential functions of identified, previously uncharacterized small proteins.

TL;DR: The results suggest that the overall pathway profiles concerned with the three stresses were similar, but the specific procedures and components in each process varied greatly, and the altered proteins and processes can be taken as effective candidates in future breeding and agronomic modification researches.

TL;DR: The results suggest that the here defined panel of CRC autoantibodies and their target proteins should be included in CRC blood-based biomarker panels to get a clinically useful blood- based diagnostic signature for CRC detection.

TL;DR: The first ever iTRAQ-based proteomic analysis of two coconut varieties in response to cold stress showed that XS (aromatic) coconut variety is cold-sensitive compared to BD (Hainan Tall) variety and increased abundance of stress response related proteins up-regulation under cold stress suggested that BD isCold-tolerant variety.

TL;DR: This review describes a number of computational approaches to utilize experimental data to glance into structure of proteins and understand their dynamics, and discusses the limitations and the resolution of the constraints-based modeling approaches.

TL;DR: This work explored the salivary transcriptome and proteome of the brown dog tick, Rhipicephalus sanguineus, confirming the "sialome switching" and validating the idea that the expression switch may serve as a mechanism of escape from the host immunity.

TL;DR: The proteomics findings reveal the roles of post-stress recovery proteins and highlight the interacting pathways responsible for bacterial cell tolerance to bile stress and improve the potential therapeutic efficacy of probiotics strains in clinical trials for patients of inflammatory bowel diseases (IBD) and related disorders.

TL;DR: It is found that the abundance of 150 proteins in high-Motility ram sperm was significantly different compared with low-motility sperm, and biomarkers were discovered to reflect the motility variation in ram spermatozoa; these biomarkers may assist in illuminating the molecular mechanisms underlying sperm motility.

TL;DR: Experimental results showed that berberine exerts its antibacterial effects against Streptococcus pyogenes by stimulated the generation of reactive oxygen species (ROS), which provides novel insights into the effect of berberines on oxidative stress as an antimicrobial drug.

TL;DR: The Western blot technique was first published 40 years ago and this anniversary represents an opportunity to look back to its origins, to address the question as to whether the technique is outdated and heading toward being replaced, and to point out current improvements.

TL;DR: The study reveals that the extent of ubiquitination at mitochondria greatly increases upon proteasome inhibition, pointing to a large number of potential substrates for proteasomal degradation.

TL;DR: New insights were gained regarding the molecular changes triggered by this disease in canine urinary proteome and metabolome, suggesting uromodulin, trigonelline and carnosine as possible biomarkers of CKD in dogs.

TL;DR: The variation between MHO and NAFLD was studied by mass spectroscopy to evaluate the mechanism with which MHO subjects resist the harmful effects induced by obesity.

TL;DR: It is supported that mature human sperm cells rely in protein phosphorylation to efficiently regulate sperm motility and the challenge of using some identified human sperm phosphorylated proteins (GSK3α) as targets to develop into clinically relevant biomarkers is raised.

TL;DR: The salivary proteome of patients with primary Sjögren's syndrome differed from that of non-pSS patients, indicating that saliva proteomics represents a promising non-invasive diagnostic tool for pSS.