Showing papers in "MethodsX in 2015"

TL;DR: A simple screening method to detect endophytic and rhizosphere bacteria, isolated from the plants in rotation with rice, for rice PGP agents based on a root colonization bioassay and a PGP trait is characterized.

TL;DR: In this paper, the authors used digital image addition and subtraction to determine the relative area of the pure collagen and live cell content in heart tissue in a semi-automated process using standard software.

TL;DR: In this article, the suitability of calcein AM for detection of intact extracellular vesicles (EVs) by flow cytometry was demonstrated, and it was used for detecting intact EVs from numerous cell types.

TL;DR: In this article, a simple and rapid colorimetric assay was developed where plant extracts are introduced to H2O2, phenol and 4-aminoantipyrine reaction system in the presence of horseradish peroxidase (HRP).

TL;DR: The protocol outlined here represents a rapid and reliable method for the generation of high-titre pseudotype viral particles with the MERS-CoV spike protein on a lentiviral core, and is adapted from previously published protocols.

TL;DR: In this paper, a simple and efficient method using proteinase K and a brief heat treatment to release RNA of quality suitable for quantitative PCR analysis was developed, which can be used directly for cDNA synthesis without purification.

TL;DR: In this paper, the authors proposed a simple method to enhance the recombinant protein expression that was optimized using several unrelated proteins, which can be used for various recombinant proteins expressed in the E. coli expression system.

TL;DR: In this article, a non-invasive imaging strategy for real-time tracking and quantification of small gold nanoparticles (sAuNPs) using a commercially available confocal laser scanning microscope is presented.

TL;DR: In this article, a static light scattering based method for quantification of virus particles without the need of virus particle standards is proposed, which is applicable for virus particles acting as Rayleigh scatterers.

TL;DR: In this paper, a simple method for 'on-line' analyses of seawater by inductively coupled plasma mass spectrometry (ICP-MS) was proposed, where excess seawater was pumped through the nebulizer of the ICP-MS during the pre-concentration step but the gas flow was adjusted so that the seawater could be pumped out as waste without being sprayed into the instrument.

TL;DR: In this article, an area fraction (AF) is used to measure the confluence of adherent monolayer cells in a non-invasive, non-destructive, label-free manner.

TL;DR: The adapted protocol will aid in future assessment of protective levels of humoral response against PEDV infection by measuring IgA levels in serum and fecal samples by identifying a strong positive correlation between the anti-PEDV levels in fecal and serum samples.

TL;DR: In this paper, a simple and reliable method for separation of rod photoreceptor perikarya without the use of staining procedures or transgenic mice was described, and an easy-follow step-by-step protocol for retina dissociation was devised.

TL;DR: In this article, a modification of the current method improves the measurement of the optical density of a solution with sulfate-reducing bacteria (SRB) cells, and the results show that the relationship between the bacterial dry mass and absorbance is exponential in the observed range.

TL;DR: In this paper, inert, stable LiCl-KCl molten salts were used as direct heat transfer fluid to separate the metal products in either liquid (solder, zinc, tin, lead, etc.) or solid (copper, gold, steel, palladium, etc.).

TL;DR: In this paper, a novel baseline estimation procedure based on previously published works is presented, where the core of the estimation is an iterative spectrum suppression consisting of a moving window minimum replacement (adapted from Friedrichs [1]).

TL;DR: In this paper, a LabVIEW-based semi-automated scoring algorithm using recorded EEG and EMG signals is presented. But the algorithm is not suitable for the automatic recognition of sleep states.

TL;DR: In this paper, an optimized method based on the coupling of two commercial kits was described for the extraction of soil nucleic acids, with simultaneous extraction and purification of DNA and RNA following a cascade scheme and avoiding the use of harmful solvents.

TL;DR: In this paper, a simple and effective silica/alginate two-step method to immobilize protoplasts with advantages in experimental manipulation and microscopic imaging, as well as in potentially studying cell biological processes such as secretion and metabolism.

TL;DR: A modified mammalian perfusion protocol for non-mammalian vertebrates often results in failed perfusions as discussed by the authors, and this protocol is easily modified for the perfusion of other tissues and for various specialized histological techniques.

TL;DR: In this paper, the authors investigated the effect of aggregation on the ELISA measurement and developed a novel chemical pre-treatment method, designed to disaggregate Aβ peptides, to improve the ILISA measurement of the total Aβ concentration.

TL;DR: In this article, a three-stage methodology for real-time identification and control of electro-mechanical actuator plants is presented, tested, and validated, and the designed controller is applied and tested on the real plant through Hardware-in-the-Loop (HIL) environment.

TL;DR: In this article, the authors proposed a new experimental set-up for flash sintering, which can be adapted to different kinds of electrolytes (samples), easily changing electrode's material and atmosphere.

TL;DR: A comparison of the existing isometric dorsiflexion protocol and the proposed improvement of the protocol which includes the fixation of the toes in addition to fixing the ankle and foot are shown.

TL;DR: In this paper, the Hardy-Schulze rule was applied to the Fe(3+) ions instead of Na(+) and K(+) for the extraction of protein from Plantago ovata seeds.

TL;DR: In this paper, a combined method using Multi-PK antibody and Phos-tag SDS-PAGE was developed for profiling the expression and phosphorylation state of intracellular protein kinases.

TL;DR: In this paper, the authors used surface plasmon resonance (SPR) to detect specific antibodies directly in multiple sclerosis patients' sera using a glucopeptide-based label free biosensor.

TL;DR: In this paper, a patch cord with FC or M3 connectors was used for long in vivo optogenetic experiments with large rodents, which can be adapted for use with other common optical-fiber connectors.

TL;DR: Fluorescent microsphere immunoassays (FMIA) are able to measure multiple cytokines in a single sample and demonstrates an increased number of detectable cytokines over published studies.