Showing papers in "Molecular and Biochemical Parasitology in 1995"

TL;DR: Overall, IRT appears to be a superb method for epidemiological and taxonomic studies of Leishmania, being sensitive, rapid and quantitative while simultaneously revealing considerable molecular diversity.

TL;DR: In certain regions of genes coding for proteins of the sporozoite and merozoite surface (CSP, TRAP, MSA-2 and PF83), dN was significantly greater than dS; thus, this unusual pattern of nucleotide substitution is indicative of positive Darwinian selection acting to promote diversity at the amino-acid level.

TL;DR: Data suggests that mefloquine resistance may have arisen by two different mechanisms in African and Southeast Asian isolates, and much more variability in the polyasparaginated region of the pfmdr1 gene was noted in this study than previously reported.

TL;DR: The data suggest that the 310- kDa form of Pfs230 arises from the cleavage of 50 kDa from the amino terminus of the 360-kDa form, which is the first report of a recombinant form of a P. falciparum gametocyte protein capable of inducing antisera that reduce malaria parasite infectivity to mosquitoes.

TL;DR: a Escola Paul&a de Medicina, Rua Botucatu, 862, CEP 04023-062, Stio Pa&o, Brazil b Institute de Quimica da USP, Sao Paula, Brazil ’ Institute de Inuestigaciones en Engenharia Genetica y Biologia Molecular, Buenos Aires.

TL;DR: These cDNA ESTs provide new avenues of research for exploring both the novel trypanosome-specific genes and the genome organization of this parasite, as well as a resource for identifying trypanOSome homologs to genes expressed in other organisms.

TL;DR: This work describes the molecular characterization of GRA6, a novel Toxoplasma gondii dense granule antigen of 32 kDa, which potentially encodes a 230-amino-acid polypeptide, does not contain any introns and seems to be present as a single copy in the genome of T. Gondii.

TL;DR: A model for this structure based on the observation that the Pfs230 molecule contains a series of regularly repeated cysteine-containing motifs is presented, which postulate that each set folds into a separate disulfide-bonded domain in which corresponding pairs of cysteines form an equivalentdisulfide bond in every such domain.

TL;DR: An axenic cultivation system to grow African trypanosomes in vitro and a novel concept considering a possible differentiation mechanism is discussed, which eventually led to a damped oscillation curve with a constant high population density after about 40 days of cultivation.

TL;DR: Comparison of the sub-cellular locations and protein sequences of Pfg377 and Pfs2 show them to be completely distinct antigens, which are proposed to play a role in parasite emergence from the erythrocyte during gametocyte maturation in the Anopheles mosquito midgut.

TL;DR: The utility of the reverse genetic approach in the study of Toxoplasma gene function is demonstrated and the ROP1 deletion mutant created by transfection with a plasmid encoding the bacterial chloramphenicol acetyltransferase (cat) gene flanked by Rop1 genomic sequences is demonstrated.

TL;DR: This report describes the use of restriction enzyme-mediated integration (REMI) to increase the transformation frequency and allow co-transfection of several unselected constructs under the selection of a single selectable marker and demonstrates that REMI may be used in the efficient stable transformation and co-transferion of this and perhaps other protozoan parasites.

TL;DR: In this paper, the authors investigated adenosine transport in the related parasite Trypanosoma equiperdum (Botat1.1) and a cloned derivative resistant to the diamidine drug berenil (diminazene aceturate) with limited crossresistance to the melaminophenyl arsenical cymelarsen.

TL;DR: The molecular cloning of a putative adhesive molecule from P. falciparum is reported that shares both sequence and structural similarities with a sporozoite surface molecule from Plasmodium termed the thrombospondin-related anonymous protein (TRAP and, to a lesser extent, with the circumsporozoite (CS) protein.

TL;DR: This research attacked the mode of transmission of Legionnaires’ disease by attacking the “spatially aggregating” immune cells of the immune system through a probiotics-like substance called “lysine”.

TL;DR: Results indicated that 24-alkyl sterols are essential growth factors for T. cruzi and that the preferred substrate of the delta 24(25) sterol methyl transferase in this organism is zymosterol.

TL;DR: PCR diagnostic assays based on allele-specific amplification that can reliably discriminate all of the mutations observed to alter codons 436, 581 and 613 should prove invaluable in further assessing the contribution of specific base changes in the DHPS gene of the parasite to the sulphadoxine resistance phenotype and to the clinical failure of the sulphADoxine/pyrimethamine combination Fansidar.

TL;DR: A gene encoding a novel cDC2-related protein kinase has been identified in Plasmodium falciparum, using degenerate oligonucleotides designed to hybridise to regions that are conserved in members of the cdc2 gene family.

TL;DR: Evidence is presented for significant parasite genotypic variability within a single endemic region of Theileria parasites and for a high prevalence of one particular RFLP, unlikely to be the result of a clonal population structure.

TL;DR: Genomic plasticity is a hallmark of many protozoan parasites, including Plasmodium spp, Trypanosoma ssp, Leishmania ssp and Giardia lamblia, and there is a common theme regarding the structural basis of this karyotype variability.

TL;DR: RNAase protection assays demonstrated that the MMP9 activity, unique to infected cells, is due to increased M MP9 mRNA levels, and transcription factor AP-1 was constitutively present in increased amounts in Theileria-infected cells.

TL;DR: The result shows that chromosomal rearrangements occur in the T. cruzi genome, albeit with a low frequency, and the information presented will make it possible to select chromosomes for the construction of physical chromosomal maps required forThe T.cruzi genome project.

TL;DR: Results of Southern blot analyses were consistent with the 3'-NT/Nu being encoded by a single copy gene, and constitute the first report of the gene for this unique trypanosomatid surface membrane enzyme.

TL;DR: This minireview attempts to provide a brief synopsis of the current understanding of the evolution and biological significance of trans-splicing.

TL;DR: The effects of 3' UTR on RNA levels and translational efficiency in T. cruzi is shown and luciferase expression was influenced by changing the steady-state level and/or the translation efficiency of luc mRNA.

TL;DR: It appears that the conventional SOD, catalase and peroxidase utilised in aerobic metabolism have been substituted in Giardia by NADH oxidase and NADH peroxideidase, similar to anaerobic bacteria.