Showing papers in "Theriogenology in 2012"

TL;DR: In this article, the authors developed flow cytometric assays to measure SO, HP, membrane lipid peroxidation, and inner mitochondrial transmembrane potential in boar sperm.

TL;DR: 2.0 μM resveratrol supplementation during IVM improved the developmental potential of PA and IVF porcine embryos by increasing the intracellular GSH level, decreasing ROS level, and regulating gene expression during oocyte maturation.

TL;DR: Addition of 5 to 20 μg/mL of either resveratrol or quercetin to the Tris-egg yolk-glycerol extender reduced sperm mitochondrial membrane potential.

TL;DR: There is much room for new research in sperm cryobiology in the three major areas outlined above-agriculture, laboratory animal medicine, and human clinical assisted reproduction, including refinement of mathematical models, optimization of cryoprotective agent addition and removal procedures for spermatozoa from many species, and development of effective, efficient, and facile cryopreservation protocols.

TL;DR: The factors of a noninfectious nature that affect the fertility of lactating dairy cows following AI are reviewed, with special attention paid to factors related to the cow and its environment and to estrus confirmation at insemination.

TL;DR: There was an additive effect of +L-carnitine +carbohydrates on early embryo development, most likely through increased β-oxidation within embryos, and it was inferred that L-c Carnitine embryos were more metabolically active, with higher rates of ATP-ADP conversion.

TL;DR: More than 90% of the variation in perinatal mortality was found at theindividual litter level and efforts to minimize puppy mortality should be targeted at the management of the individual litter rather than at the breed level.

TL;DR: The present study showed that the activity of CAT, GPx and SOD in chicken semen was affected by cryopreservation, what increased the intensity of lipid peroxidation (LPO).

TL;DR: Both esters of estradiol administered either at P4 device removal (EC) or 24 h later (EB) were effective in inducing an LH surge which resulted in synchronized ovulations and similar P/AI in suckled Bos indicus beef cows submitted to TAI.

TL;DR: This review presents a comparative guide of classical and recent advances in PCR-based methods for avian molecular sexing, highlighting its strengths and limitations and future research opportunities in this field are addressed.

TL;DR: It has been concluded that over 25 yr with no direct evidence of disease transmission by transferred cryopreserved human and animal embryos, that the presentCryopreservation technology is sanitary sound, with the stipulation that biocontainment measures recommended by the International Embryo Transfer Society and the World Organization for Animal Health are strictly followed.

TL;DR: The addition of α-tocopherol and ascorbic acid to the extender media, together with the antioxidant system of the spermatozoa improved sea bass sperm motility, which is one of the impairment parameters most affected by cryopreservation.

TL;DR: A better understanding of the reprogramming mechanism will have a direct impact on the efficiency of current SCNT procedures, as well as iPSC derivation.

TL;DR: The iPS cells provided a unique source of pluripotent cells with utility in conservation through cryopreservation of genetics, as a source of reprogrammed donor cells for nuclear transfer or for directed differentiation to gametes in the future.

TL;DR: These reprogrammed siPSCs may constitute a robust pluripotent alternative to elusive sheep ESCs, with great potential for use in agriculture and pharmaceutical biotechnology.

TL;DR: Theendometrial cytobrush technique was successfully used to obtain material for both cytology and RNA extraction, and il8 gene expression may be useful to predict endometrial inflammation.

TL;DR: A comprehensive overview of proteomics and associated techniques and technologies (such as separations and mass spectrometry) is given and some opportunities for application of these tools in the area of reproduction are suggested.

TL;DR: The toxicity of glycerol is apparently related to osmotic and nonosmotic effects and the concentration in the freezing media for stallion spermatozoa should not surpass 2.5%.

TL;DR: It is demonstrated that L-carnitine treatment during in vitro maturation (IVM) of immature pig oocytes improves the developmental competence of SCNT embryos.

TL;DR: Even though the cytobrush technique requires specialized equipment, sample collection by this method was easier, more consistent, and quicker than the lavage method, indicating that the brush would be the preferred collection method for use on field in the mare.

TL;DR: Differences in vocabulary, culture, and research methodologies has hampered knowledge translation and represent a systemic impediment to a broad understanding of ovarian function and limits progress and innovation in the development of safer and more efficacious treatments for infertility and contraception.

TL;DR: Repeated 'snap shots' every 2 mo would allow AI centers to adjust number of sperm per AI straw from genomic-sires in a timely manner, to maintain high pregnancy rates, and to meet market demands with sires producing ∼40% as many sperm as mature 'proven sires' of yesteryear.

TL;DR: Exogenous melatonin acts via receptors and has important roles in regulation of development and function of bovine GCs and is regulated differently by melatonin in time- and dose-dependent manners.

TL;DR: Although semen motility is considered an important parameter to validate the quality of the ejaculate processed, it only minimally relates to fertility results under the current Dutch AI practice.

TL;DR: Evaluated pregnancy per AI of dairy cows subjected to the 5-day timed AI protocol under various synchronization and luteolytic treatments and found presynchronization with G6G increased the proportion of cows with a CL on Day -8 and ovulation to the first GnRH of the protocol.

TL;DR: It is shown that it is possible to obtain similar postthaw sperm motility and pregnancy rates using GLY or EG as a cryoprotectant for donkey semen, and that in the conditions of this study the re-extension in SPL of thawed semen before AI showed a trend toward the improvement of fertility and increased PMN concentration in uterine flushings.

TL;DR: A simple, field friendly and effective method to attain highly concentrated semen samples with excellent motility in lions and potentially other wild felid species as an alternative to electroejaculation is described.

TL;DR: 8% MF and both 5% and 8% DMF were the cryoprotectants with the best postthaw quality for C. macropomum sperm, and both fertilization and hatching rates were similar to those with fresh sperm.

TL;DR: In mares, HR remained constant during the last days of pregnancy but decreased within one day after parturition, while maternal HRV did not change, indicating increasing demands on the cardiovascular system with adaptation to extrauterine life.

TL;DR: Characterizing the biophysical traits of gametes and tissues is the most efficient way to develop successful storage and recovery protocols, but, such data are only available for a few laboratory, livestock, and fish species, with virtually all others having gone unstudied.