Journal ArticleDOI
5-(3-carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)-3-(4-sulfophenyl)tetrazolium, inner salt (MTS) and related analogs of 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) reducing to purple water-soluble formazans As cell-viability indicators
Reads0
Chats0
TLDR
Analogs of MTT, 3-(4,5-dimethylthiazolyl)-2,5diphenyltetrazolium bromide, designed to yield water-soluble formazans upon reduction, have been synthesized and evaluated as cell-viability indicators as mentioned in this paper.About:
This article is published in Bioorganic & Medicinal Chemistry Letters.The article was published on 1991-01-01. It has received 411 citations till now.read more
Citations
More filters
Book ChapterDOI
Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction.
TL;DR: Second generation tetrazolium dyes that form water-soluble formazans and require an intermediate electron acceptor for reduction (XTT, WST-1 and to some extent, MTS), are characterised by a net negative charge and are therefore largely cell-impermeable and considerable evidence indicates that their reduction occurs at the cell surface or at the level of the plasma membrane via trans-plasma membrane electron transport.
Cell Viability Assays
Terry L. Riss,Richard A. Moravec,Andrew L. Niles,Sarah Duellman,Hélène A Benink,Tracy J Worzella,Lisa Minor +6 more
TL;DR: These assays are used for measuring the results of cell proliferation, testing for cytotoxic effects of compounds, and for multiplexing as an internal control to determine viable cell number during other cell-based assays.
Journal ArticleDOI
The sensitivity and specificity of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines
TL;DR: Since the sensitivity of the MTS assay might be increased using different cell types or by extended incubation, this assay is found to provide ideal features of a good measurement system that might also be used for on site toxicological assessments.
Journal ArticleDOI
Biomaterials functionalization using a novel peptide that selectively binds to a conducting polymer.
TL;DR: This strategy for surface functionalization of an electrically conductive polymer, chlorine-doped polypyrrole, which has been widely researched for various electronic and biomedical applications, is developed and can be extended to immobilize a variety of molecules to PPyCl for numerous applications.
Journal ArticleDOI
Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell lines
TL;DR: Its advantages over XTT/PMS, another tetrazolium which yields a water-soluble formazan product, include the absorbance range of color produced, the rapidity of color development, and the storage stability of the MTS/P MS reagent solution.
References
More filters
Journal Article
Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay
Michael C. Alley,Dominic A. Scudiero,Anne Monks,Miriam L. Hursey,Maciej Czerwinski,Donald L. Fine,B. J. Abbott,Joseph G. Mayo,Robert H. Shoemaker,Michael R. Boyd +9 more
TL;DR: Since the microculture tetrazolium assay provides sensitive and reproducible indices of growth as well as drug sensitivity in individual cell lines over the course of multiple passages and several months' cultivation, it appears suitable for initial-stage in vitro drug screening.
Journal Article
Evaluation of a Soluble Tetrazolium/Formazan Assay for Cell Growth and Drug Sensitivity in Culture Using Human and Other Tumor Cell Lines
Dominic A. Scudiero,Robert H. Shoemaker,Kenneth D. Paull,Anne Monks,Siobhan Tierney,Thomas H. Nofziger,Michael J. Currens,Donna Seniff,Michael R. Boyd +8 more
TL;DR: The new XTT reagent provides for a simplified, in vitro cell growth assay with possible applicability to a variety of problems in cellular pharmacology and biology, but still shares many of the limitations and potential pitfalls of MTT or other tetrazolium-based assays.
Journal ArticleDOI
New Soluble-Formazan Assay for HIV-1 Cytopathic Effects: Application to High-Flux Screening of Synthetic and Natural Products for AIDS-Antiviral Activity
Owen S. Weislow,Rebecca Kiser,Donald L. Fine,John P. Bader,Robert H. Shoemaker,Michael R. Boyd +5 more
TL;DR: In this paper, an effective and optimally safe microculture method for rapid and convenient assay of the in vitro cytopathic effects of human immunodeficiency virus (HIV-1) on human lymphoblastoid or other suitable host cells.
Journal Article
Effects of the pH dependence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide-formazan absorption on chemosensitivity determined by a novel tetrazolium-based assay
TL;DR: It is shown that reduction of M TT to MTT-formazan by cells is dependent on the amount of MTT in the incubation medium and can be used to estimate cell numbers in a simple chemosensitivity assay the results of which agree well with a commonly used clonogenic assay.
Journal ArticleDOI
Rapid colormetric assay for cell viability: Application to the quantitation of cytotoxic and growth inhibitory lymphokines
TL;DR: The data indicate that the L929 cell line was 10-50-fold more sensitive than the ESH -5L line to the lytic activity of cytotoxins produced by human phytohemagglutinin-P-activated T lymphocytes, or the cytotoxin produced by peripheral blood lymphocytes stimulated with various tumor cell lines.
Related Papers (5)
Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture.
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction
M.V. Berridge,A.S. Tan +1 more