[6n]methyl adenine in the nuclear dna of a eucaryote, tetrahymena pyriformis
TLDR
DNA isolated from macronuclei of the ciliate, Tetrahymena pyriformis, has been found to contain [6N]methyl adenine (MeAde); this represents the first clear demonstration of significant amounts of MeAde in the DNA of a eucaryote.Abstract:
DNA isolated from macronuclei of the ciliate, Tetrahymena pyriformis, has been found to contain [6N]methyl adenine (MeAde); this represents the first clear demonstration of significant amounts of MeAde in the DNA of a eucaryote. The amounts of macronuclear MeAde differed slightly between different strains of Tetrahymena, with approximately 0.65–0.80% of the adenine bases being methylated. The MeAde content of macronuclear DNA did not seem to vary in different physiological states. The level of MeAde in DNA isolated from micronuclei, on the other hand, was quite low (at least tenfold lower than in macronuclear DNA).read more
Citations
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Journal ArticleDOI
DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
Book ChapterDOI
Isolation of micro- and macronuclei of Tetrahymena pyriformis.
TL;DR: This chapter discusses the isolation of micro- and macronuclei of Tetrahymena Pyriformis and suggests that these two nuclei may be used as a model system to explore the mechanisms whereby the same genetic information is maintained in different structural and functional states in eukaryotic cells.
Journal ArticleDOI
N6-methyladenine: the other methylated base of DNA
TL;DR: Interestingly, even if adenine methylation is usually considered a bacterial DNA feature, the presence of m6A has been found in protist and plant DNAs, highlighting the importance of considering m 6A as the sixth element of DNA.
Journal ArticleDOI
DNA N 6 -methyladenine: a new epigenetic mark in eukaryotes?
TL;DR: Recent publications documenting the presence of 6mA in Chlamydomonas reinhardtii, Drosophila melanogaster and Caenorhabditis elegans are discussed and possible roles for this DNA modification in regulating transcription, the activity of transposable elements and transgenerational epigenetic inheritance are considered.
Journal ArticleDOI
Comparison of the sequences of macro- and micronuclear DNA of Tetrahymena pyriformis
Meng-Chao Yao,Martin A. Gorovsky +1 more
TL;DR: Findings place severe constraint on possible models concerning the structure of the Tetrahymena macronucleus, and are very different from the situation observed in Stylonychia where it has been suggested that only a small percentage of the sequences in micronuclei are present in significant amounts in macron nuclei.
References
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Journal ArticleDOI
Regulierung der DNS-Menge im Makronucleus von Tetrahymena
TL;DR: The amount of Feulgen-positive material in the macronucleus of Tetrahymena pyriformis HSM was determined by means of microphotometry in different stages of the cell cycle and in cell lines to maintain the mean DNA amount and the degree of variation.